Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.).
<h4>Background</h4>Several small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and γ-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pec...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/6553a770801a43ffb528770ea53688b0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:6553a770801a43ffb528770ea53688b0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:6553a770801a43ffb528770ea53688b02021-11-18T07:21:18ZQuorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.).1932-620310.1371/journal.pone.0035176https://doaj.org/article/6553a770801a43ffb528770ea53688b02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539957/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Several small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and γ-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pectobacterium species including recent or emerging potato isolates.<h4>Methodology/principal findings</h4>Using bacterial biosensors, immunoassay, and chromatographic analysis, we showed that soft-rot bacteria have the common ability to produce transiently during their exponential phase of growth the N-3-oxo-hexanoyl- or the N-3-oxo-octanoyl-l-homoserine lactones and a molecule of the autoinducer-2 family. Dickeya spp. produced in addition the indole-3-acetic acid in tryptophan-rich conditions. All these signaling molecules have been identified for the first time in the novel Dickeya solani species. In contrast, quinolone and γ-amino butyrate signals were not identified and the corresponding synthases are not present in the available genomes of soft-rot bacteria. To determine if the variations of signal production according to growth phase could result from expression modifications of the corresponding synthase gene, the respective mRNA levels were estimated by reverse transcriptase-PCR. While the N-acyl-homoserine lactone production is systematically correlated to the synthase expression, that of the autoinducer-2 follows the expression of an enzyme upstream in the activated methyl cycle and providing its precursor, rather than the expression of its own synthase.<h4>Conclusions/significance</h4>Despite sharing the S-adenosylmethionine precursor, no strong link was detected between the production kinetics or metabolic pathways of autoinducers-1 and -2. In contrast, the signaling pathway of autoinducer-2 seems to be switched off by the indole-3-acetic acid pathway under tryptophan control. It therefore appears that the two genera of soft-rot bacteria have similarities but also differences in the mechanisms of communication via the diffusible molecules. Our results designate autoinducer-1 lactones as the main targets for a global biocontrol of soft-rot bacteria communications, including those of emerging isolates.Alexandre CrépinCorinne BarbeyAmélie Beury-CirouValérie HéliasLaure TaupinSylvie ReverchonWilliam NasserDenis FaureAlain DufourNicole OrangeMarc FeuilloleyKarin HeurlierJean-François BuriniXavier LatourPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35176 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Alexandre Crépin Corinne Barbey Amélie Beury-Cirou Valérie Hélias Laure Taupin Sylvie Reverchon William Nasser Denis Faure Alain Dufour Nicole Orange Marc Feuilloley Karin Heurlier Jean-François Burini Xavier Latour Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| description |
<h4>Background</h4>Several small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and γ-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pectobacterium species including recent or emerging potato isolates.<h4>Methodology/principal findings</h4>Using bacterial biosensors, immunoassay, and chromatographic analysis, we showed that soft-rot bacteria have the common ability to produce transiently during their exponential phase of growth the N-3-oxo-hexanoyl- or the N-3-oxo-octanoyl-l-homoserine lactones and a molecule of the autoinducer-2 family. Dickeya spp. produced in addition the indole-3-acetic acid in tryptophan-rich conditions. All these signaling molecules have been identified for the first time in the novel Dickeya solani species. In contrast, quinolone and γ-amino butyrate signals were not identified and the corresponding synthases are not present in the available genomes of soft-rot bacteria. To determine if the variations of signal production according to growth phase could result from expression modifications of the corresponding synthase gene, the respective mRNA levels were estimated by reverse transcriptase-PCR. While the N-acyl-homoserine lactone production is systematically correlated to the synthase expression, that of the autoinducer-2 follows the expression of an enzyme upstream in the activated methyl cycle and providing its precursor, rather than the expression of its own synthase.<h4>Conclusions/significance</h4>Despite sharing the S-adenosylmethionine precursor, no strong link was detected between the production kinetics or metabolic pathways of autoinducers-1 and -2. In contrast, the signaling pathway of autoinducer-2 seems to be switched off by the indole-3-acetic acid pathway under tryptophan control. It therefore appears that the two genera of soft-rot bacteria have similarities but also differences in the mechanisms of communication via the diffusible molecules. Our results designate autoinducer-1 lactones as the main targets for a global biocontrol of soft-rot bacteria communications, including those of emerging isolates. |
| format |
article |
| author |
Alexandre Crépin Corinne Barbey Amélie Beury-Cirou Valérie Hélias Laure Taupin Sylvie Reverchon William Nasser Denis Faure Alain Dufour Nicole Orange Marc Feuilloley Karin Heurlier Jean-François Burini Xavier Latour |
| author_facet |
Alexandre Crépin Corinne Barbey Amélie Beury-Cirou Valérie Hélias Laure Taupin Sylvie Reverchon William Nasser Denis Faure Alain Dufour Nicole Orange Marc Feuilloley Karin Heurlier Jean-François Burini Xavier Latour |
| author_sort |
Alexandre Crépin |
| title |
Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| title_short |
Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| title_full |
Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| title_fullStr |
Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| title_full_unstemmed |
Quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (Dickeya and Pectobacterium spp.). |
| title_sort |
quorum sensing signaling molecules produced by reference and emerging soft-rot bacteria (dickeya and pectobacterium spp.). |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/6553a770801a43ffb528770ea53688b0 |
| work_keys_str_mv |
AT alexandrecrepin quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT corinnebarbey quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT ameliebeurycirou quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT valeriehelias quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT lauretaupin quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT sylviereverchon quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT williamnasser quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT denisfaure quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT alaindufour quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT nicoleorange quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT marcfeuilloley quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT karinheurlier quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT jeanfrancoisburini quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp AT xavierlatour quorumsensingsignalingmoleculesproducedbyreferenceandemergingsoftrotbacteriadickeyaandpectobacteriumspp |
| _version_ |
1718423601167728640 |