Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability

Haitao Luo,1 Bingbing Jiang,2 Bingyun Li,2&ndash;4 Zhaoliang Li,1 Bing-Hua Jiang,5 Yi Charlie Chen11Department of Biology, Natural Science Division, Alderson-Broaddus College, Philippi, 2Department of Orthopaedics, School of Medicine, West Virginia University, 3WVNano Initiative, 4Mary Babb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luo H, Jiang B, Li B, Li Z, Jiang BH, Chen YC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65563d9ebaad47daa50103bb3a4581ae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65563d9ebaad47daa50103bb3a4581ae
record_format	dspace
spelling	oai:doaj.org-article:65563d9ebaad47daa50103bb3a4581ae2021-12-02T05:49:32ZKaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability1176-91141178-2013https://doaj.org/article/65563d9ebaad47daa50103bb3a4581ae2012-07-01T00:00:00Zhttp://www.dovepress.com/kaempferol-nanoparticles-achieve-strong-and-selective-inhibition-of-ov-a10494https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Haitao Luo,1 Bingbing Jiang,2 Bingyun Li,2&ndash;4 Zhaoliang Li,1 Bing-Hua Jiang,5 Yi Charlie Chen11Department of Biology, Natural Science Division, Alderson-Broaddus College, Philippi, 2Department of Orthopaedics, School of Medicine, West Virginia University, 3WVNano Initiative, 4Mary Babb Randolph Cancer Center, Morgantown, WV, USA; 5Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USAAbstract: Ovarian cancer is one of the leading causes of cancer death for women throughout the Western world. Kaempferol, a natural flavonoid, has shown promise in the chemoprevention of ovarian cancer. A common concern about using dietary supplements for chemoprevention is their bioavailability. Nanoparticles have shown promise in increasing the bioavailability of some chemicals. Here we developed five different types of nanoparticles incorporating kaempferol and tested their efficacy in the inhibition of viability of cancerous and normal ovarian cells. We found that positively charged nanoparticle formulations did not lead to a significant reduction in cancer cell viability, whereas nonionic polymeric nanoparticles resulted in enhanced reduction of cancer cell viability. Among the nonionic polymeric nanoparticles, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) nanoparticles incorporating kaempferol led to significant reduction in cell viability of both cancerous and normal cells. Poly(DL-lactic acid-co-glycolic acid) (PLGA) nanoparticles incorporating kaempferol resulted in enhanced reduction of cancer cell viability together with no significant reduction in cell viability of normal cells compared with kaempferol alone. Therefore, both PEO-PPO-PEO and PLGA nanoparticle formulations were effective in reducing cancer cell viability, while PLGA nanoparticles incorporating kaempferol had selective toxicity against cancer cells and normal cells. A PLGA nanoparticle formulation could be advantageous in the prevention and treatment of ovarian cancers. On the other hand, PEO-PPO-PEO nanoparticles incorporating kaempferol were more effective inhibitors of cancer cells, but they also significantly reduced the viability of normal cells. PEO-PPO-PEO nanoparticles incorporating kaempferol may be suitable as a cancer-targeting strategy, which could limit the effects of the nanoparticles on normal cells while retaining their potency against cancer cells. We have identified two nanoparticle formulations incorporating kaempferol that may lead to breakthroughs in cancer treatment. Both PEO-PPO-PEO and PLGA nanoparticle formulations had superior effects compared with kaempferol alone in reducing cancer cell viability.Keywords: nanochemoprevention, kaempferol, ovarian cancer, nanoparticles, viability, natural compoundLuo HJiang BLi BLi ZJiang BHChen YCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3951-3959 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Luo H Jiang B Li B Li Z Jiang BH Chen YC Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
description	Haitao Luo,1 Bingbing Jiang,2 Bingyun Li,2&ndash;4 Zhaoliang Li,1 Bing-Hua Jiang,5 Yi Charlie Chen11Department of Biology, Natural Science Division, Alderson-Broaddus College, Philippi, 2Department of Orthopaedics, School of Medicine, West Virginia University, 3WVNano Initiative, 4Mary Babb Randolph Cancer Center, Morgantown, WV, USA; 5Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USAAbstract: Ovarian cancer is one of the leading causes of cancer death for women throughout the Western world. Kaempferol, a natural flavonoid, has shown promise in the chemoprevention of ovarian cancer. A common concern about using dietary supplements for chemoprevention is their bioavailability. Nanoparticles have shown promise in increasing the bioavailability of some chemicals. Here we developed five different types of nanoparticles incorporating kaempferol and tested their efficacy in the inhibition of viability of cancerous and normal ovarian cells. We found that positively charged nanoparticle formulations did not lead to a significant reduction in cancer cell viability, whereas nonionic polymeric nanoparticles resulted in enhanced reduction of cancer cell viability. Among the nonionic polymeric nanoparticles, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) nanoparticles incorporating kaempferol led to significant reduction in cell viability of both cancerous and normal cells. Poly(DL-lactic acid-co-glycolic acid) (PLGA) nanoparticles incorporating kaempferol resulted in enhanced reduction of cancer cell viability together with no significant reduction in cell viability of normal cells compared with kaempferol alone. Therefore, both PEO-PPO-PEO and PLGA nanoparticle formulations were effective in reducing cancer cell viability, while PLGA nanoparticles incorporating kaempferol had selective toxicity against cancer cells and normal cells. A PLGA nanoparticle formulation could be advantageous in the prevention and treatment of ovarian cancers. On the other hand, PEO-PPO-PEO nanoparticles incorporating kaempferol were more effective inhibitors of cancer cells, but they also significantly reduced the viability of normal cells. PEO-PPO-PEO nanoparticles incorporating kaempferol may be suitable as a cancer-targeting strategy, which could limit the effects of the nanoparticles on normal cells while retaining their potency against cancer cells. We have identified two nanoparticle formulations incorporating kaempferol that may lead to breakthroughs in cancer treatment. Both PEO-PPO-PEO and PLGA nanoparticle formulations had superior effects compared with kaempferol alone in reducing cancer cell viability.Keywords: nanochemoprevention, kaempferol, ovarian cancer, nanoparticles, viability, natural compound
format	article
author	Luo H Jiang B Li B Li Z Jiang BH Chen YC
author_facet	Luo H Jiang B Li B Li Z Jiang BH Chen YC
author_sort	Luo H
title	Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
title_short	Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
title_full	Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
title_fullStr	Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
title_full_unstemmed	Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
title_sort	kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/65563d9ebaad47daa50103bb3a4581ae
work_keys_str_mv	AT luoh kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability AT jiangb kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability AT lib kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability AT liz kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability AT jiangbh kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability AT chenyc kaempferolnanoparticlesachievestrongandselectiveinhibitionofovariancancercellviability
_version_	1718400232715190272

Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability

Ejemplares similares