CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation

Abstract Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We...

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Autores principales:	Pankaj Attri, Jeongmin Han, Sooho Choi, Eun Ha Choi, Annemie Bogaerts, Weontae Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6557944bd4654ca4a25f449f93ce0eac
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spelling	oai:doaj.org-article:6557944bd4654ca4a25f449f93ce0eac2021-12-02T15:09:05ZCAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation10.1038/s41598-018-28600-w2045-2322https://doaj.org/article/6557944bd4654ca4a25f449f93ce0eac2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28600-whttps://doaj.org/toc/2045-2322Abstract Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.Pankaj AttriJeongmin HanSooho ChoiEun Ha ChoiAnnemie BogaertsWeontae LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pankaj Attri Jeongmin Han Sooho Choi Eun Ha Choi Annemie Bogaerts Weontae Lee CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
description	Abstract Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.
format	article
author	Pankaj Attri Jeongmin Han Sooho Choi Eun Ha Choi Annemie Bogaerts Weontae Lee
author_facet	Pankaj Attri Jeongmin Han Sooho Choi Eun Ha Choi Annemie Bogaerts Weontae Lee
author_sort	Pankaj Attri
title	CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
title_short	CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
title_full	CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
title_fullStr	CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
title_full_unstemmed	CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
title_sort	cap modifies the structure of a model protein from thermophilic bacteria: mechanisms of cap-mediated inactivation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6557944bd4654ca4a25f449f93ce0eac
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CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation

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