Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model

Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model

Di Shi,1 Gujie Mi,1 Soumya Bhattacharya,2 Suprabha Nayar,2 Thomas J Webster1,3 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Materials Science and Technology Division, Council for Scientific and Industrial Research-National Metallurgical Laboratory, Jamshedpur, Ind...

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Autores principales: Shi D, Mi G, Bhattacharya S, Nayar S, Webster TJ
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
SPIONs
blood-brain barrier
permeability
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/655e9586bfa94b9d80888e667cea2e23
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spelling oai:doaj.org-article:655e9586bfa94b9d80888e667cea2e232021-12-02T05:03:01ZOptimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model1178-2013https://doaj.org/article/655e9586bfa94b9d80888e667cea2e232016-10-01T00:00:00Zhttps://www.dovepress.com/optimizing-superparamagnetic-iron-oxide-nanoparticles-as-drug-carriers-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Di Shi,1 Gujie Mi,1 Soumya Bhattacharya,2 Suprabha Nayar,2 Thomas J Webster1,3 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Materials Science and Technology Division, Council for Scientific and Industrial Research-National Metallurgical Laboratory, Jamshedpur, India; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: In the current study, an optimized in vitro blood–brain barrier (BBB) model was established using mouse brain endothelial cells (b.End3) and astrocytes (C8-D1A). Before measuring the permeability of superparamagnetic iron oxide nanoparticle (SPION) samples, the BBB was first examined and confirmed by an immunofluorescent stain and evaluating the transendothelial electrical resistance. After such confirmation, the permeability of the following five previously synthesized SPIONs was determined using this optimized BBB model: 1) GGB (synthesized using glycine, glutamic acid, and bovine serum albumin [BSA]), 2) GGC (glycine, glutamic acid, and collagen), 3) GGP (glycine, glutamic acid, and polyvinyl alcohol), 4) BPC (BSA, polyethylene glycol, and collagen), and 5) CPB (collagen, polyvinyl alcohol, and BSA). More importantly, after the permeability test, transmission electron microscopy thin section technology was used to investigate the mechanism behind this process. Transmission electron microscopy thin section images supported the hypothesis that collagen-coated CPB SPIONs displayed better cellular uptake than glycine and glutamine acid-coated GGB SPIONs. Such experimental data demonstrated how one can modify SPIONs to better deliver drugs to the brain to treat a wide range of neurological disorders. Keywords: superparamagnetic iron oxide nanoparticles, blood–brain barrier, permeabilityShi DMi GBhattacharya SNayar SWebster TJDove Medical PressarticleSPIONsblood-brain barrierpermeabilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5371-5379 (2016)
institution DOAJ
collection DOAJ
language EN
topic SPIONs
blood-brain barrier
permeability
Medicine (General)
R5-920
spellingShingle SPIONs
blood-brain barrier
permeability
Medicine (General)
R5-920
Shi D
Mi G
Bhattacharya S
Nayar S
Webster TJ
Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
description Di Shi,1 Gujie Mi,1 Soumya Bhattacharya,2 Suprabha Nayar,2 Thomas J Webster1,3 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Materials Science and Technology Division, Council for Scientific and Industrial Research-National Metallurgical Laboratory, Jamshedpur, India; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: In the current study, an optimized in vitro blood–brain barrier (BBB) model was established using mouse brain endothelial cells (b.End3) and astrocytes (C8-D1A). Before measuring the permeability of superparamagnetic iron oxide nanoparticle (SPION) samples, the BBB was first examined and confirmed by an immunofluorescent stain and evaluating the transendothelial electrical resistance. After such confirmation, the permeability of the following five previously synthesized SPIONs was determined using this optimized BBB model: 1) GGB (synthesized using glycine, glutamic acid, and bovine serum albumin [BSA]), 2) GGC (glycine, glutamic acid, and collagen), 3) GGP (glycine, glutamic acid, and polyvinyl alcohol), 4) BPC (BSA, polyethylene glycol, and collagen), and 5) CPB (collagen, polyvinyl alcohol, and BSA). More importantly, after the permeability test, transmission electron microscopy thin section technology was used to investigate the mechanism behind this process. Transmission electron microscopy thin section images supported the hypothesis that collagen-coated CPB SPIONs displayed better cellular uptake than glycine and glutamine acid-coated GGB SPIONs. Such experimental data demonstrated how one can modify SPIONs to better deliver drugs to the brain to treat a wide range of neurological disorders. Keywords: superparamagnetic iron oxide nanoparticles, blood–brain barrier, permeability
format article
author Shi D
Mi G
Bhattacharya S
Nayar S
Webster TJ
author_facet Shi D
Mi G
Bhattacharya S
Nayar S
Webster TJ
author_sort Shi D
title Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
title_short Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
title_full Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
title_fullStr Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
title_full_unstemmed Optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
title_sort optimizing superparamagnetic iron oxide nanoparticles as drug carriers using an in vitro blood–brain barrier model
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/655e9586bfa94b9d80888e667cea2e23
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AT bhattacharyas optimizingsuperparamagneticironoxidenanoparticlesasdrugcarriersusinganinvitrobloodndashbrainbarriermodel
AT nayars optimizingsuperparamagneticironoxidenanoparticlesasdrugcarriersusinganinvitrobloodndashbrainbarriermodel
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