The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway
Tripterygium wilfordii is a vine from the Celastraceae family that is used in traditional Chinese medicine (TCM). The active ingredient, celastrol, is a friedelane-type pentacyclic triterpenoid with putative roles as an antitumor, immunosuppressive, and anti-obesity agent. Here, we repo...
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oai:doaj.org-article:6560e0344df24f1cb2b9110b86c3902f2021-12-02T15:00:33ZThe genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway10.46471/gigabyte.142709-4715https://doaj.org/article/6560e0344df24f1cb2b9110b86c3902f2021-02-01T00:00:00Zhttps://gigabytejournal.com/articles/14https://doaj.org/toc/2709-4715 Tripterygium wilfordii is a vine from the Celastraceae family that is used in traditional Chinese medicine (TCM). The active ingredient, celastrol, is a friedelane-type pentacyclic triterpenoid with putative roles as an antitumor, immunosuppressive, and anti-obesity agent. Here, we report a reference genome assembly of T. wilfordii with high-quality annotation using a hybrid sequencing strategy. The total genome size obtained is 340.12 Mb, with a contig N50 value of 3.09 Mb. We successfully anchored 91.02% of sequences into 23 pseudochromosomes using high-throughput chromosome conformation capture (Hi–C) technology. The super-scaffold N50 value was 13.03 Mb. We also annotated 31,593 structural genes, with a repeat percentage of 44.31%. These data demonstrate that T. wilfordii diverged from Malpighiales species approximately 102.4 million years ago. By integrating genome, transcriptome and metabolite analyses, as well as in vivo and in vitro enzyme assays of two cytochrome P450 (CYP450) genes, TwCYP712K1 and TwCYP712K2, it is possible to investigate the second biosynthesis step of celastrol and demonstrate that this was derived from a common ancestor. These data provide insights and resources for further investigation of pathways related to celastrol, and valuable information to aid the conservation of resources, as well as understand the evolution of Celastrales. Tianlin PeiMengxiao YanYu KongHang FanJie LiuMengying CuiYumin FangBinjie GeJun YangQing ZhaoGigaScience PressarticleElectronic computers. Computer scienceQA75.5-76.95ENGigaByte (2021) |
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Electronic computers. Computer science QA75.5-76.95 |
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Electronic computers. Computer science QA75.5-76.95 Tianlin Pei Mengxiao Yan Yu Kong Hang Fan Jie Liu Mengying Cui Yumin Fang Binjie Ge Jun Yang Qing Zhao The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| description |
Tripterygium wilfordii is a vine from the Celastraceae family that is used in traditional Chinese medicine (TCM). The active ingredient, celastrol, is a friedelane-type pentacyclic triterpenoid with putative roles as an antitumor, immunosuppressive, and anti-obesity agent. Here, we report a reference genome assembly of T. wilfordii with high-quality annotation using a hybrid sequencing strategy. The total genome size obtained is 340.12 Mb, with a contig N50 value of 3.09 Mb. We successfully anchored 91.02% of sequences into 23 pseudochromosomes using high-throughput chromosome conformation capture (Hi–C) technology. The super-scaffold N50 value was 13.03 Mb. We also annotated 31,593 structural genes, with a repeat percentage of 44.31%. These data demonstrate that T. wilfordii diverged from Malpighiales species approximately 102.4 million years ago. By integrating genome, transcriptome and metabolite analyses, as well as in vivo and in vitro enzyme assays of two cytochrome P450 (CYP450) genes, TwCYP712K1 and TwCYP712K2, it is possible to investigate the second biosynthesis step of celastrol and demonstrate that this was derived from a common ancestor. These data provide insights and resources for further investigation of pathways related to celastrol, and valuable information to aid the conservation of resources, as well as understand the evolution of Celastrales.
|
| format |
article |
| author |
Tianlin Pei Mengxiao Yan Yu Kong Hang Fan Jie Liu Mengying Cui Yumin Fang Binjie Ge Jun Yang Qing Zhao |
| author_facet |
Tianlin Pei Mengxiao Yan Yu Kong Hang Fan Jie Liu Mengying Cui Yumin Fang Binjie Ge Jun Yang Qing Zhao |
| author_sort |
Tianlin Pei |
| title |
The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| title_short |
The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| title_full |
The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| title_fullStr |
The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| title_full_unstemmed |
The genome of Tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| title_sort |
genome of tripterygium wilfordii and characterization of the celastrol biosynthesis pathway |
| publisher |
GigaScience Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/6560e0344df24f1cb2b9110b86c3902f |
| work_keys_str_mv |
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