Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria

Beehives are populated by bacterial species with a protective role against honey bee pathogens thanks to the production of bioactive metabolites. These compounds are largely unexploited despite their high potential interest for pest management. This study evaluated the capability of bacterial specie...

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Autores principales:	Maria Ludovica Saccà, Giulia Bianchi, Roberto Lo Scalzo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	natural compounds chemical signal microbial communication biosynthetic potential honey bee microbiota <i>Varroa destructor</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/65650da287f5466aa8f1491dd4826d94
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spelling	oai:doaj.org-article:65650da287f5466aa8f1491dd4826d942021-11-25T18:24:20ZBiosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria10.3390/microorganisms91122182076-2607https://doaj.org/article/65650da287f5466aa8f1491dd4826d942021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2218https://doaj.org/toc/2076-2607Beehives are populated by bacterial species with a protective role against honey bee pathogens thanks to the production of bioactive metabolites. These compounds are largely unexploited despite their high potential interest for pest management. This study evaluated the capability of bacterial species associated with honey bees to produce 2-heptanone, a volatile organic compound with anesthetic properties of the parasitic mite <i>Varroa destructor</i>. The production of this compound was quantified by SPME-GC-MS in a culture filtrate of nine bacterial strains isolated from the surface of honey bees, and the biosynthetic potential was evaluated in bacterial species associated with apiaries by searching for protein homologs putatively involved in its biosynthesis by using biocomputational tools. The findings pointed out that 2-heptanone was produced by <i>Acetobacteraceae bacterium</i>, <i>Bacillus thuringiensis</i> and <i>Apilactobacillus kunkeei</i> isolates in concentrations between 1.5 and 2.6 ng/mL and that its production was strain-specific. Putative methylketone synthase homologs were found in <i>Bacillus</i>, <i>Gilliamella</i>, <i>Acetobacteraceae</i>, <i>Bartonella</i> and Lactobacillaceae, and the protein sequence results were distributed in nine Sequence Similarity Network (SSN) clusters. These preliminary results support the hypothesis that 2-heptanone may act as a mediator of microbial relationships in hives and provide contributions to assess the role and biosynthetic potential of 2-heptanone in apiaries.Maria Ludovica SaccàGiulia BianchiRoberto Lo ScalzoMDPI AGarticlenatural compoundschemical signalmicrobial communicationbiosynthetic potentialhoney bee microbiota<i>Varroa destructor</i>Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2218, p 2218 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	natural compounds chemical signal microbial communication biosynthetic potential honey bee microbiota <i>Varroa destructor</i> Biology (General) QH301-705.5
spellingShingle	natural compounds chemical signal microbial communication biosynthetic potential honey bee microbiota <i>Varroa destructor</i> Biology (General) QH301-705.5 Maria Ludovica Saccà Giulia Bianchi Roberto Lo Scalzo Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
description	Beehives are populated by bacterial species with a protective role against honey bee pathogens thanks to the production of bioactive metabolites. These compounds are largely unexploited despite their high potential interest for pest management. This study evaluated the capability of bacterial species associated with honey bees to produce 2-heptanone, a volatile organic compound with anesthetic properties of the parasitic mite <i>Varroa destructor</i>. The production of this compound was quantified by SPME-GC-MS in a culture filtrate of nine bacterial strains isolated from the surface of honey bees, and the biosynthetic potential was evaluated in bacterial species associated with apiaries by searching for protein homologs putatively involved in its biosynthesis by using biocomputational tools. The findings pointed out that 2-heptanone was produced by <i>Acetobacteraceae bacterium</i>, <i>Bacillus thuringiensis</i> and <i>Apilactobacillus kunkeei</i> isolates in concentrations between 1.5 and 2.6 ng/mL and that its production was strain-specific. Putative methylketone synthase homologs were found in <i>Bacillus</i>, <i>Gilliamella</i>, <i>Acetobacteraceae</i>, <i>Bartonella</i> and Lactobacillaceae, and the protein sequence results were distributed in nine Sequence Similarity Network (SSN) clusters. These preliminary results support the hypothesis that 2-heptanone may act as a mediator of microbial relationships in hives and provide contributions to assess the role and biosynthetic potential of 2-heptanone in apiaries.
format	article
author	Maria Ludovica Saccà Giulia Bianchi Roberto Lo Scalzo
author_facet	Maria Ludovica Saccà Giulia Bianchi Roberto Lo Scalzo
author_sort	Maria Ludovica Saccà
title	Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
title_short	Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
title_full	Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
title_fullStr	Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
title_full_unstemmed	Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria
title_sort	biosynthesis of 2-heptanone, a volatile organic compound with a protective role against honey bee pathogens, by hive associated bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/65650da287f5466aa8f1491dd4826d94
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Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria

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