Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel

Bing Yang1,2*, ChangYang Gong1*, Xia Zhao2, ShengTao Zhou2, ZhengYu Li2, XiaoRong Qi2, Qian Zhong2, Feng Luo1, ZhiYong Qian11State Key Laboratory of Biotherapy, West China University Hospital, Sichuan University, Chengdu, People&#39;s Republic of China; 2Department of Gynecology and Obstetri...

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Autores principales:	Yang B, Gong C, Zhao X, Zhou S, Li Z, Qi X, Zhong Q, Luo F, Qian Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/656e579f81254820bf0e3aa113e0b97c
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spelling	oai:doaj.org-article:656e579f81254820bf0e3aa113e0b97c2021-12-02T00:40:24ZPreventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel1176-91141178-2013https://doaj.org/article/656e579f81254820bf0e3aa113e0b97c2012-02-01T00:00:00Zhttp://www.dovepress.com/preventing-postoperative-abdominal-adhesions-in-a-rat-model-with-peg-p-a9193https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Bing Yang1,2, ChangYang Gong1, Xia Zhao2, ShengTao Zhou2, ZhengYu Li2, XiaoRong Qi2, Qian Zhong2, Feng Luo1, ZhiYong Qian11State Key Laboratory of Biotherapy, West China University Hospital, Sichuan University, Chengdu, People&#39;s Republic of China; 2Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People&#39;s Republic of China*These authors contributed equally in this workBackground: Poly (ethylene glycol)-poly (&epsilon;-caprolactone)-poly (ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel has been demonstrated to be biocompatible and thermosensitive. In this study, its potential efficacy and mechanisms of preventing postsurgical abdominal adhesions were investigated.Results: PECE hydrogel was transformed into gel state from sol state in less than 20 seconds at 37&deg;C. None of the animals treated with the hydrogel (n = 15) developed adhesions. In contrast, all untreated animals (n = 15) had adhesions that could only be separated by sharp dissection (P &lt; 0.001). The hydrogel adhered to the peritoneal wounds, gradually disappeared from the wounds within 7 days, and transformed into viscous fluid, being completely absorbed within 12 days. The parietal and visceral peritoneum were remesothelialized in about 5 and 9 days, respectively. The hydrogel prevented the formation of fibrinous adhesion and the invasion of fibroblasts. Also, along with the hydrogel degradation, a temporary inflammatory cell barrier was formed which could effectively delay the invasion of fibroblasts during the critical period of mesothelial regeneration.Conclusion: The results suggested that PECE hydrogel could effectively prevent postsurgical intra-abdominal adhesions, which possibly result from the prevention of the fibrinous adhesion formation and the fibroblast invasion, the promotion of the remesothelialization, and the hydroflotation effect.Keywords: anti-adhesion, thermosensitive, barrier, biocompatibleYang BGong CZhao XZhou SLi ZQi XZhong QLuo FQian ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 547-557 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang B Gong C Zhao X Zhou S Li Z Qi X Zhong Q Luo F Qian Z Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
description	Bing Yang1,2, ChangYang Gong1, Xia Zhao2, ShengTao Zhou2, ZhengYu Li2, XiaoRong Qi2, Qian Zhong2, Feng Luo1, ZhiYong Qian11State Key Laboratory of Biotherapy, West China University Hospital, Sichuan University, Chengdu, People&#39;s Republic of China; 2Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People&#39;s Republic of China*These authors contributed equally in this workBackground: Poly (ethylene glycol)-poly (&epsilon;-caprolactone)-poly (ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel has been demonstrated to be biocompatible and thermosensitive. In this study, its potential efficacy and mechanisms of preventing postsurgical abdominal adhesions were investigated.Results: PECE hydrogel was transformed into gel state from sol state in less than 20 seconds at 37&deg;C. None of the animals treated with the hydrogel (n = 15) developed adhesions. In contrast, all untreated animals (n = 15) had adhesions that could only be separated by sharp dissection (P &lt; 0.001). The hydrogel adhered to the peritoneal wounds, gradually disappeared from the wounds within 7 days, and transformed into viscous fluid, being completely absorbed within 12 days. The parietal and visceral peritoneum were remesothelialized in about 5 and 9 days, respectively. The hydrogel prevented the formation of fibrinous adhesion and the invasion of fibroblasts. Also, along with the hydrogel degradation, a temporary inflammatory cell barrier was formed which could effectively delay the invasion of fibroblasts during the critical period of mesothelial regeneration.Conclusion: The results suggested that PECE hydrogel could effectively prevent postsurgical intra-abdominal adhesions, which possibly result from the prevention of the fibrinous adhesion formation and the fibroblast invasion, the promotion of the remesothelialization, and the hydroflotation effect.Keywords: anti-adhesion, thermosensitive, barrier, biocompatible
format	article
author	Yang B Gong C Zhao X Zhou S Li Z Qi X Zhong Q Luo F Qian Z
author_facet	Yang B Gong C Zhao X Zhou S Li Z Qi X Zhong Q Luo F Qian Z
author_sort	Yang B
title	Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
title_short	Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
title_full	Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
title_fullStr	Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
title_full_unstemmed	Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel
title_sort	preventing postoperative abdominal adhesions in a rat model with peg-pcl-peg hydrogel
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/656e579f81254820bf0e3aa113e0b97c
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Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel

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