Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water

Per- and polyfluoroalkyl substances (PFAS) are widely detected environmental contaminants, and there is a great need for development of sensor technologies for rapid and continuous monitoring of PFAS. In this study, we have developed fluorescence based aptasensor that can possibly monitor perfluoroo...

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Autores principales:	Junyoung Park, Kyung-Ae Yang, Yongju Choi, Jong Kwon Choe
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Perfluoroalkyl substance Fluorescence-based aptasensor Binding mechanism Perfluorooctanoic acid detection Water monitoring Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/65715f55a6ce46e79f33d86c7c022262
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spelling	oai:doaj.org-article:65715f55a6ce46e79f33d86c7c0222622021-11-26T04:23:38ZNovel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water0160-412010.1016/j.envint.2021.107000https://doaj.org/article/65715f55a6ce46e79f33d86c7c0222622022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0160412021006255https://doaj.org/toc/0160-4120Per- and polyfluoroalkyl substances (PFAS) are widely detected environmental contaminants, and there is a great need for development of sensor technologies for rapid and continuous monitoring of PFAS. In this study, we have developed fluorescence based aptasensor that can possibly monitor perfluorooctanoic acid (PFOA) in water with limit of detection (LOD) of 0.17 μM. This is first to report the successful isolation of PFAS binding ssDNA aptamers. The obtained aptamer selectively binds PFOA with dissociation constant (KD) of 5.5 μM. Specific aptamer binding sites to PFOA were identified and the length of the fluorinated carbons was a key binding factor rather than the functional group. The aptamer binding to structurally similar PFAS compounds (i.e., perfluorocarboxylic acids and perfluorosulfonic acids with 4–8 carbon chains) was also investigated; the aptamer KD values were 6.5 and 3.3 μM for perfluoroheptanoic acid and perfluorohexanesulfonic acid, respectively, while other analogs did not bind to the aptamer. The presence of major inorganic ions and dissolved organic matter had negligible influences on the aptamer performance (<14% at a 10 mM concentration), and the aptamer performance was also robust in real wastewater effluent conditions, with a KD of 7.4 μM for PFOA. Fluorescence-based aptasensor developed in this study is adequate in monitoring PFOA levels in water contaminated with the accident spills and heavy usage of fire-fighting foams near the industrial sites and military bases. More importantly, the study opens up new capability of aptasensors to efficiently monitor the trace amount of various PFAS compounds and other fluorinated alternatives in natural and engineered water environments.Junyoung ParkKyung-Ae YangYongju ChoiJong Kwon ChoeElsevierarticlePerfluoroalkyl substanceFluorescence-based aptasensorBinding mechanismPerfluorooctanoic acid detectionWater monitoringEnvironmental sciencesGE1-350ENEnvironment International, Vol 158, Iss , Pp 107000- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Perfluoroalkyl substance Fluorescence-based aptasensor Binding mechanism Perfluorooctanoic acid detection Water monitoring Environmental sciences GE1-350
spellingShingle	Perfluoroalkyl substance Fluorescence-based aptasensor Binding mechanism Perfluorooctanoic acid detection Water monitoring Environmental sciences GE1-350 Junyoung Park Kyung-Ae Yang Yongju Choi Jong Kwon Choe Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
description	Per- and polyfluoroalkyl substances (PFAS) are widely detected environmental contaminants, and there is a great need for development of sensor technologies for rapid and continuous monitoring of PFAS. In this study, we have developed fluorescence based aptasensor that can possibly monitor perfluorooctanoic acid (PFOA) in water with limit of detection (LOD) of 0.17 μM. This is first to report the successful isolation of PFAS binding ssDNA aptamers. The obtained aptamer selectively binds PFOA with dissociation constant (KD) of 5.5 μM. Specific aptamer binding sites to PFOA were identified and the length of the fluorinated carbons was a key binding factor rather than the functional group. The aptamer binding to structurally similar PFAS compounds (i.e., perfluorocarboxylic acids and perfluorosulfonic acids with 4–8 carbon chains) was also investigated; the aptamer KD values were 6.5 and 3.3 μM for perfluoroheptanoic acid and perfluorohexanesulfonic acid, respectively, while other analogs did not bind to the aptamer. The presence of major inorganic ions and dissolved organic matter had negligible influences on the aptamer performance (<14% at a 10 mM concentration), and the aptamer performance was also robust in real wastewater effluent conditions, with a KD of 7.4 μM for PFOA. Fluorescence-based aptasensor developed in this study is adequate in monitoring PFOA levels in water contaminated with the accident spills and heavy usage of fire-fighting foams near the industrial sites and military bases. More importantly, the study opens up new capability of aptasensors to efficiently monitor the trace amount of various PFAS compounds and other fluorinated alternatives in natural and engineered water environments.
format	article
author	Junyoung Park Kyung-Ae Yang Yongju Choi Jong Kwon Choe
author_facet	Junyoung Park Kyung-Ae Yang Yongju Choi Jong Kwon Choe
author_sort	Junyoung Park
title	Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
title_short	Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
title_full	Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
title_fullStr	Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
title_full_unstemmed	Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
title_sort	novel ssdna aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/65715f55a6ce46e79f33d86c7c022262
work_keys_str_mv	AT junyoungpark novelssdnaaptamerbasedfluorescencesensorforperfluorooctanoicaciddetectioninwater AT kyungaeyang novelssdnaaptamerbasedfluorescencesensorforperfluorooctanoicaciddetectioninwater AT yongjuchoi novelssdnaaptamerbasedfluorescencesensorforperfluorooctanoicaciddetectioninwater AT jongkwonchoe novelssdnaaptamerbasedfluorescencesensorforperfluorooctanoicaciddetectioninwater
_version_	1718409905212227584

Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water

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