The proteome of the human endolymphatic sac endolymph

Abstract The endolymphatic sac (ES) is the third part of the inner ear, along with the cochlea and vestibular apparatus. A refined sampling technique was developed to analyse the proteomics of ES endolymph. With a tailored solid phase micro-extraction probe, five ES endolymph samples were collected,...

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Autores principales:	Christine Ölander, Jesper Edvardsson Rasmussen, Per Olof Eriksson, Göran Laurell, Helge Rask-Andersen, Jonas Bergquist
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6571bad6f4f64b12a083a15bfd7b8998
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spelling	oai:doaj.org-article:6571bad6f4f64b12a083a15bfd7b89982021-12-02T17:51:22ZThe proteome of the human endolymphatic sac endolymph10.1038/s41598-021-89597-32045-2322https://doaj.org/article/6571bad6f4f64b12a083a15bfd7b89982021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89597-3https://doaj.org/toc/2045-2322Abstract The endolymphatic sac (ES) is the third part of the inner ear, along with the cochlea and vestibular apparatus. A refined sampling technique was developed to analyse the proteomics of ES endolymph. With a tailored solid phase micro-extraction probe, five ES endolymph samples were collected, and six sac tissue biopsies were obtained in patients undergoing trans-labyrinthine surgery for sporadic vestibular schwannoma. The samples were analysed using nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) to identify the total number of proteins. Pathway identification regarding molecular function and protein class was presented. A total of 1656 non-redundant proteins were identified, with 1211 proteins detected in the ES endolymph. A total of 110 proteins were unique to the ES endolymph. The results from the study both validate a strategy for in vivo and in situ human sampling during surgery and may also form a platform for further investigations to better understand the function of this intriguing part of the inner ear.Christine ÖlanderJesper Edvardsson RasmussenPer Olof ErikssonGöran LaurellHelge Rask-AndersenJonas BergquistNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christine Ölander Jesper Edvardsson Rasmussen Per Olof Eriksson Göran Laurell Helge Rask-Andersen Jonas Bergquist The proteome of the human endolymphatic sac endolymph
description	Abstract The endolymphatic sac (ES) is the third part of the inner ear, along with the cochlea and vestibular apparatus. A refined sampling technique was developed to analyse the proteomics of ES endolymph. With a tailored solid phase micro-extraction probe, five ES endolymph samples were collected, and six sac tissue biopsies were obtained in patients undergoing trans-labyrinthine surgery for sporadic vestibular schwannoma. The samples were analysed using nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) to identify the total number of proteins. Pathway identification regarding molecular function and protein class was presented. A total of 1656 non-redundant proteins were identified, with 1211 proteins detected in the ES endolymph. A total of 110 proteins were unique to the ES endolymph. The results from the study both validate a strategy for in vivo and in situ human sampling during surgery and may also form a platform for further investigations to better understand the function of this intriguing part of the inner ear.
format	article
author	Christine Ölander Jesper Edvardsson Rasmussen Per Olof Eriksson Göran Laurell Helge Rask-Andersen Jonas Bergquist
author_facet	Christine Ölander Jesper Edvardsson Rasmussen Per Olof Eriksson Göran Laurell Helge Rask-Andersen Jonas Bergquist
author_sort	Christine Ölander
title	The proteome of the human endolymphatic sac endolymph
title_short	The proteome of the human endolymphatic sac endolymph
title_full	The proteome of the human endolymphatic sac endolymph
title_fullStr	The proteome of the human endolymphatic sac endolymph
title_full_unstemmed	The proteome of the human endolymphatic sac endolymph
title_sort	proteome of the human endolymphatic sac endolymph
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6571bad6f4f64b12a083a15bfd7b8998
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The proteome of the human endolymphatic sac endolymph

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