Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells

Rui Liu,1 Qiang Pei,2 Tao Shou,1 Wenjing Zhang,1 Jing Hu,1 Wei Li31Department of Oncology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of China; 2Department of Hematology, The First People’s Hospital of Yunnan Province, Kun...

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Autores principales:	Liu R, Pei Q, Shou T, Zhang W, Hu J, Li W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Curcuma wenyujin CWAuNPs Renal Cancer A498 Anticancer agent Apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6573709a6915461f8c706f7cb16e75de
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spelling	oai:doaj.org-article:6573709a6915461f8c706f7cb16e75de2021-12-02T08:07:44ZApoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells1178-2013https://doaj.org/article/6573709a6915461f8c706f7cb16e75de2019-06-01T00:00:00Zhttps://www.dovepress.com/apoptotic-effect-of-green-synthesized-gold-nanoparticles-from-curcuma--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rui Liu,1 Qiang Pei,2 Tao Shou,1 Wenjing Zhang,1 Jing Hu,1 Wei Li31Department of Oncology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of China; 2Department of Hematology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of China; 3Department of Urinary Surgery, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of ChinaIntroduction: Curcuma wenyujin is a plant which belongs to the family of Zingiberaceae, found in South Asia and China. C. wenyujin is a major constituent in Chinese traditional medicine and is used to treat liver diseases, blood clots, and is also prescribed as a painkiller. C. wenyujin possesses antioxidant, antiproliferative, and antitumorogenic properties, and many researchers have proved the efficacy of C. wenyujin against various types of cancer. The major drawback of this historical drug is it’s low bioavailability.Methods: This study synthesized gold nanoparticles using C. wenyujin and assessed its potency against in vitro renal cancer cells. The biosynthesized C. wenyujin gold nanoparticles (CWAuNPs) were characterized using UV-Spec, DLS, FTIR, SAED, TEM, EDAX, and Atomic Force analysis. The cytotoxicity of CWAuNPs against renal cancer cell lines A498 and SW-156 was assessed with MTT assay. The induction of apoptosis by CWAuNPs in A498 cell was measured using apoptotic staining DAPI, Rhodamine 123, and H2DCFDA. The apoptotic activity of CWAuNPs was further confirmed with flow cytometric analysis. The molecular mechanism of CWAuNPs was analyzed with qPCR and immunoblotting analysis of caspases, proapoptotic, and antiapoptotic proteins.Results: The characterization of results of synthesized CWAuNPs satisfy the distinctive properties of a potent nanodrug. The results of apoptotic staining techniques confirm the induction of CWAuNPs in A498 by increasing the apoptotic Caspase 3,9, Bid, and Bad, and decreasing the antiapoptotic protein Bcl-2, Bcl-xl expressions, which is authentically proven by the qPCR and immunoblotting analysis.Conclusion: In conclusion, these results confirmed that biosynthesized CWAuNPs is a potent anticancer agent which induces apoptosis in the A498 renal carcinoma cell line.Keywords: Curcuma wenyujin, CWAuNPs, renal cancer, A498, anticancer agent, apoptosisLiu RPei QShou TZhang WHu JLi WDove Medical PressarticleCurcuma wenyujinCWAuNPsRenal CancerA498Anticancer agentApoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4091-4103 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Curcuma wenyujin CWAuNPs Renal Cancer A498 Anticancer agent Apoptosis Medicine (General) R5-920
spellingShingle	Curcuma wenyujin CWAuNPs Renal Cancer A498 Anticancer agent Apoptosis Medicine (General) R5-920 Liu R Pei Q Shou T Zhang W Hu J Li W Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
description	Rui Liu,1 Qiang Pei,2 Tao Shou,1 Wenjing Zhang,1 Jing Hu,1 Wei Li31Department of Oncology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of China; 2Department of Hematology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of China; 3Department of Urinary Surgery, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, People’s Republic of ChinaIntroduction: Curcuma wenyujin is a plant which belongs to the family of Zingiberaceae, found in South Asia and China. C. wenyujin is a major constituent in Chinese traditional medicine and is used to treat liver diseases, blood clots, and is also prescribed as a painkiller. C. wenyujin possesses antioxidant, antiproliferative, and antitumorogenic properties, and many researchers have proved the efficacy of C. wenyujin against various types of cancer. The major drawback of this historical drug is it’s low bioavailability.Methods: This study synthesized gold nanoparticles using C. wenyujin and assessed its potency against in vitro renal cancer cells. The biosynthesized C. wenyujin gold nanoparticles (CWAuNPs) were characterized using UV-Spec, DLS, FTIR, SAED, TEM, EDAX, and Atomic Force analysis. The cytotoxicity of CWAuNPs against renal cancer cell lines A498 and SW-156 was assessed with MTT assay. The induction of apoptosis by CWAuNPs in A498 cell was measured using apoptotic staining DAPI, Rhodamine 123, and H2DCFDA. The apoptotic activity of CWAuNPs was further confirmed with flow cytometric analysis. The molecular mechanism of CWAuNPs was analyzed with qPCR and immunoblotting analysis of caspases, proapoptotic, and antiapoptotic proteins.Results: The characterization of results of synthesized CWAuNPs satisfy the distinctive properties of a potent nanodrug. The results of apoptotic staining techniques confirm the induction of CWAuNPs in A498 by increasing the apoptotic Caspase 3,9, Bid, and Bad, and decreasing the antiapoptotic protein Bcl-2, Bcl-xl expressions, which is authentically proven by the qPCR and immunoblotting analysis.Conclusion: In conclusion, these results confirmed that biosynthesized CWAuNPs is a potent anticancer agent which induces apoptosis in the A498 renal carcinoma cell line.Keywords: Curcuma wenyujin, CWAuNPs, renal cancer, A498, anticancer agent, apoptosis
format	article
author	Liu R Pei Q Shou T Zhang W Hu J Li W
author_facet	Liu R Pei Q Shou T Zhang W Hu J Li W
author_sort	Liu R
title	Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
title_short	Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
title_full	Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
title_fullStr	Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
title_full_unstemmed	Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells
title_sort	apoptotic effect of green synthesized gold nanoparticles from curcuma wenyujin extract against human renal cell carcinoma a498 cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6573709a6915461f8c706f7cb16e75de
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Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells

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