Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue

Abstract Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in hum...

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Autores principales:	Limor Man, Laura Park, Richard Bodine, Michael Ginsberg, Nikica Zaninovic, Omar Alexander Man, Glenn Schattman, Zev Rosenwaks, Daylon James
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6574bc83056645219f3525b0523282f2
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spelling	oai:doaj.org-article:6574bc83056645219f3525b0523282f22021-12-02T11:52:28ZEngineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue10.1038/s41598-017-08491-z2045-2322https://doaj.org/article/6574bc83056645219f3525b0523282f22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08491-zhttps://doaj.org/toc/2045-2322Abstract Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.Limor ManLaura ParkRichard BodineMichael GinsbergNikica ZaninovicOmar Alexander ManGlenn SchattmanZev RosenwaksDaylon JamesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Limor Man Laura Park Richard Bodine Michael Ginsberg Nikica Zaninovic Omar Alexander Man Glenn Schattman Zev Rosenwaks Daylon James Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
description	Abstract Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.
format	article
author	Limor Man Laura Park Richard Bodine Michael Ginsberg Nikica Zaninovic Omar Alexander Man Glenn Schattman Zev Rosenwaks Daylon James
author_facet	Limor Man Laura Park Richard Bodine Michael Ginsberg Nikica Zaninovic Omar Alexander Man Glenn Schattman Zev Rosenwaks Daylon James
author_sort	Limor Man
title	Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
title_short	Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
title_full	Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
title_fullStr	Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
title_full_unstemmed	Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
title_sort	engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6574bc83056645219f3525b0523282f2
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Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue

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