Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH

Laccases are multi-copper oxidases that use molecular oxygen as the electron acceptor to oxidize phenolic and indirectly also non-phenolic substrates by mechanisms involving radicals. Due to their eco-friendliness and broad substrate specificity, laccases span a wide range of biotechnological applic...

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Autores principales: Jussi Kontro, Christina Lyra, Milla Koponen, Jaana Kuuskeri, Mika A. Kähkönen, Janne Wallenius, Xing Wan, Jussi Sipilä, Miia R. Mäkelä, Paula Nousiainen, Kristiina Hildén
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Publicado: Frontiers Media S.A. 2021
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spelling oai:doaj.org-article:6576a7fb1c3d49cb9905ace965c94d8f2021-11-09T06:20:37ZProduction of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH2296-418510.3389/fbioe.2021.767139https://doaj.org/article/6576a7fb1c3d49cb9905ace965c94d8f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.767139/fullhttps://doaj.org/toc/2296-4185Laccases are multi-copper oxidases that use molecular oxygen as the electron acceptor to oxidize phenolic and indirectly also non-phenolic substrates by mechanisms involving radicals. Due to their eco-friendliness and broad substrate specificity, laccases span a wide range of biotechnological applications. We have heterologously expressed a laccase from the coprophilic basidiomycete Coprinopsis cinerea (CcLcc9) in the methylotrophic yeast Pichia pastoris. The recombinant CcLcc9 (rCcLcc9) oxidized 2,6-dimethoxyphenol in the neutral pH range, and showed thermostability up to 70°C. The rCcLcc9 efficiently oxidized veratryl alcohol to veratraldehyde in the presence of low molecular weight mediators syringyl nitrile, methyl syringate and violuric acid, which are syringyl-type plant phenolics that have shown potential as natural co-oxidants for lignocellulosic materials. In addition, rCcLcc9 is able to depolymerize biorefinery hardwood lignin in the presence of methyl syringate and syringyl nitrile as indicated by gel permeation chromatography, and infrared spectral and nucleic magnetic resonance analyses. Furthermore, we showed that several added-value aromatic compounds, such as vanillin, vanillic acid, syringaldehyde, syringic acid and p-hydroxybenzoic acid, were formed during sequential biocatalytic chemical degradation of biorefinery lignin, indicating that rCcLcc9 harbors a great potential for sustainable processes of circular economy and modern biorefineries.Jussi KontroChristina LyraMilla KoponenJaana KuuskeriMika A. KähkönenJanne WalleniusXing WanJussi SipiläMiia R. MäkeläPaula NousiainenKristiina HildénFrontiers Media S.A.articleCoprinopsis cinerealaccase characteristicslaccase-mediator systemslignin depolymerizationstructural analysisBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Coprinopsis cinerea
laccase characteristics
laccase-mediator systems
lignin depolymerization
structural analysis
Biotechnology
TP248.13-248.65
spellingShingle Coprinopsis cinerea
laccase characteristics
laccase-mediator systems
lignin depolymerization
structural analysis
Biotechnology
TP248.13-248.65
Jussi Kontro
Christina Lyra
Milla Koponen
Jaana Kuuskeri
Mika A. Kähkönen
Janne Wallenius
Xing Wan
Jussi Sipilä
Miia R. Mäkelä
Paula Nousiainen
Kristiina Hildén
Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
description Laccases are multi-copper oxidases that use molecular oxygen as the electron acceptor to oxidize phenolic and indirectly also non-phenolic substrates by mechanisms involving radicals. Due to their eco-friendliness and broad substrate specificity, laccases span a wide range of biotechnological applications. We have heterologously expressed a laccase from the coprophilic basidiomycete Coprinopsis cinerea (CcLcc9) in the methylotrophic yeast Pichia pastoris. The recombinant CcLcc9 (rCcLcc9) oxidized 2,6-dimethoxyphenol in the neutral pH range, and showed thermostability up to 70°C. The rCcLcc9 efficiently oxidized veratryl alcohol to veratraldehyde in the presence of low molecular weight mediators syringyl nitrile, methyl syringate and violuric acid, which are syringyl-type plant phenolics that have shown potential as natural co-oxidants for lignocellulosic materials. In addition, rCcLcc9 is able to depolymerize biorefinery hardwood lignin in the presence of methyl syringate and syringyl nitrile as indicated by gel permeation chromatography, and infrared spectral and nucleic magnetic resonance analyses. Furthermore, we showed that several added-value aromatic compounds, such as vanillin, vanillic acid, syringaldehyde, syringic acid and p-hydroxybenzoic acid, were formed during sequential biocatalytic chemical degradation of biorefinery lignin, indicating that rCcLcc9 harbors a great potential for sustainable processes of circular economy and modern biorefineries.
format article
author Jussi Kontro
Christina Lyra
Milla Koponen
Jaana Kuuskeri
Mika A. Kähkönen
Janne Wallenius
Xing Wan
Jussi Sipilä
Miia R. Mäkelä
Paula Nousiainen
Kristiina Hildén
author_facet Jussi Kontro
Christina Lyra
Milla Koponen
Jaana Kuuskeri
Mika A. Kähkönen
Janne Wallenius
Xing Wan
Jussi Sipilä
Miia R. Mäkelä
Paula Nousiainen
Kristiina Hildén
author_sort Jussi Kontro
title Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
title_short Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
title_full Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
title_fullStr Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
title_full_unstemmed Production of Recombinant Laccase From Coprinopsis cinerea and Its Effect in Mediator Promoted Lignin Oxidation at Neutral pH
title_sort production of recombinant laccase from coprinopsis cinerea and its effect in mediator promoted lignin oxidation at neutral ph
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/6576a7fb1c3d49cb9905ace965c94d8f
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