Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

Our aim was to evaluate immunomodulatory properties of an original bioflavonoid complex in experimental immune disturbances induced by cyclophosphamide (Cy). We have studied morphometric indexes of thymus and spleen, as well as blood leukocyte counts, cell proliferative activity in lymphoid organs,...

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Autores principales:	I. A. Goldina, E. V. Markova, I. A. Orlovskaya, L. B. Toporkova, V. A. Kozlov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	bioflavonoids hemopoietic stem cells immune cells proliferative activity immune response cyclophosphamide immuno suppression Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/657c9927a95f4944b26f8106eb776263
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spelling	oai:doaj.org-article:657c9927a95f4944b26f8106eb7762632021-11-18T08:03:50ZCorrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders1563-06252313-741X10.15789/1563-0625-CEO-2072https://doaj.org/article/657c9927a95f4944b26f8106eb7762632021-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2072https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XOur aim was to evaluate immunomodulatory properties of an original bioflavonoid complex in experimental immune disturbances induced by cyclophosphamide (Cy). We have studied morphometric indexes of thymus and spleen, as well as blood leukocyte counts, cell proliferative activity in lymphoid organs, delayed hypersensitivity responses to T cell-dependent antigen, along with differentiation activity of bone marrow stem cells in experimental animals during Cy-induced immune suppression after a course of bioflavonoid treatment. Suspension of the bioflafonoid complex was introduced to the male mice (СВАхC57Bl/6)F1 aged 12- 14 weeks at a daily dose of 2 mg/animal (80 mg/kg), per os, using gastric catheter, over 14 days. Cytostatic immunosuppression was produced by a single intraperitoneal Cy injection. Proliferative activity of spleen and thymic cells was determined by standard method with Н3 -thymidine incorporation in the 72-h cell culture. Cellular immune response was assayed by the degree of delayed-type hypersensitivity development in response to sheep erythrocytes. The number of hematopoietic progenitors was evaluated by culturing bone marrow cells in methylcellulose-based medium. The experiments have shown mitigation of immunosuppressive effects induced by Cy, in the course of bioflavonoid complex treatment, with respect to absolute and relative mass of lymphoid organs and leukocyte numbers in peripheral blood. Moreover, we have demonstrated decreased effects of Cy treatment upon the spontaneous activity of spleen cells, mitogen-induced thymocyte and splenocyte proliferation, intensivity of delayed-type hypersensitivity response that reached the values of intact animals. Following the course of bioflavonoids, we have revealed an increase in early hematopoietic progenitors. Alleviation of Cy-induced suppressive effects upon cellular immune response, proliferation rates of immune cells, as well as stimulation of hematopoietic stem cell functions suggest a sufficient capacity of the original bioflavonoid complex for modulation of immunity and hematopoiesis, thus presenting experimental proofs for its potential usage as an adjuvant treatment of the patients with malignant diseases.I. A. GoldinaE. V. MarkovaI. A. OrlovskayaL. B. ToporkovaV. A. KozlovSPb RAACIarticlebioflavonoidshemopoietic stem cellsimmune cellsproliferative activityimmune responsecyclophosphamideimmuno suppressionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 6, Pp 1111-1120 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	bioflavonoids hemopoietic stem cells immune cells proliferative activity immune response cyclophosphamide immuno suppression Immunologic diseases. Allergy RC581-607
spellingShingle	bioflavonoids hemopoietic stem cells immune cells proliferative activity immune response cyclophosphamide immuno suppression Immunologic diseases. Allergy RC581-607 I. A. Goldina E. V. Markova I. A. Orlovskaya L. B. Toporkova V. A. Kozlov Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
description	Our aim was to evaluate immunomodulatory properties of an original bioflavonoid complex in experimental immune disturbances induced by cyclophosphamide (Cy). We have studied morphometric indexes of thymus and spleen, as well as blood leukocyte counts, cell proliferative activity in lymphoid organs, delayed hypersensitivity responses to T cell-dependent antigen, along with differentiation activity of bone marrow stem cells in experimental animals during Cy-induced immune suppression after a course of bioflavonoid treatment. Suspension of the bioflafonoid complex was introduced to the male mice (СВАхC57Bl/6)F1 aged 12- 14 weeks at a daily dose of 2 mg/animal (80 mg/kg), per os, using gastric catheter, over 14 days. Cytostatic immunosuppression was produced by a single intraperitoneal Cy injection. Proliferative activity of spleen and thymic cells was determined by standard method with Н3 -thymidine incorporation in the 72-h cell culture. Cellular immune response was assayed by the degree of delayed-type hypersensitivity development in response to sheep erythrocytes. The number of hematopoietic progenitors was evaluated by culturing bone marrow cells in methylcellulose-based medium. The experiments have shown mitigation of immunosuppressive effects induced by Cy, in the course of bioflavonoid complex treatment, with respect to absolute and relative mass of lymphoid organs and leukocyte numbers in peripheral blood. Moreover, we have demonstrated decreased effects of Cy treatment upon the spontaneous activity of spleen cells, mitogen-induced thymocyte and splenocyte proliferation, intensivity of delayed-type hypersensitivity response that reached the values of intact animals. Following the course of bioflavonoids, we have revealed an increase in early hematopoietic progenitors. Alleviation of Cy-induced suppressive effects upon cellular immune response, proliferation rates of immune cells, as well as stimulation of hematopoietic stem cell functions suggest a sufficient capacity of the original bioflavonoid complex for modulation of immunity and hematopoiesis, thus presenting experimental proofs for its potential usage as an adjuvant treatment of the patients with malignant diseases.
format	article
author	I. A. Goldina E. V. Markova I. A. Orlovskaya L. B. Toporkova V. A. Kozlov
author_facet	I. A. Goldina E. V. Markova I. A. Orlovskaya L. B. Toporkova V. A. Kozlov
author_sort	I. A. Goldina
title	Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
title_short	Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
title_full	Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
title_fullStr	Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
title_full_unstemmed	Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
title_sort	corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/657c9927a95f4944b26f8106eb776263
work_keys_str_mv	AT iagoldina correctiveeffectsoforiginalbioflavonoidcomplexinthecyclophosphamideinducedimmunitydisorders AT evmarkova correctiveeffectsoforiginalbioflavonoidcomplexinthecyclophosphamideinducedimmunitydisorders AT iaorlovskaya correctiveeffectsoforiginalbioflavonoidcomplexinthecyclophosphamideinducedimmunitydisorders AT lbtoporkova correctiveeffectsoforiginalbioflavonoidcomplexinthecyclophosphamideinducedimmunitydisorders AT vakozlov correctiveeffectsoforiginalbioflavonoidcomplexinthecyclophosphamideinducedimmunitydisorders
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Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

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