Application of electric potential improves ethanol production from xylose by active sludge

Abstract Background Low-cost raw materials such as lignocellulosic materials have been utilized in second-generation ethanol production process. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial lignocellu...

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Autores principales:	Lei Chen, Mingpeng Wang, Zhaojie Zhang, Yujie Feng
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Xylose consumption Ethanol production Electro-fermentation Extracellular electron transfer Microbial community structure Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/657db429a2a64b34812e0df71eb09f6b
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spelling	oai:doaj.org-article:657db429a2a64b34812e0df71eb09f6b2021-11-21T12:25:40ZApplication of electric potential improves ethanol production from xylose by active sludge10.1186/s13068-021-02065-y1754-6834https://doaj.org/article/657db429a2a64b34812e0df71eb09f6b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02065-yhttps://doaj.org/toc/1754-6834Abstract Background Low-cost raw materials such as lignocellulosic materials have been utilized in second-generation ethanol production process. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial lignocellulosic biorefinery. Results By applying different constant potentials to different microbial electrolysis cells with xylose as the sole carbon source, we analyzed the output of metabolites, microbial community structures, electron flow, and carbon flow in the process of xylose electro-fermentation by domesticated activated sludge. The bioreactors produced currents when applying positive potentials. The peak currents of the + 0.242 V, + 0.542 V and + 0.842 V reactors were 0.96 × 10–6 A, 3.36 × 10–6 A and 6.43 × 10–6 A, respectively. The application of potentials promoted the xylose consumption, and the maximum consumption rate in the + 0.542 V reactor was 95.5%, which was 34.8 times that of the reactor without applied potential. The potential application also promoted the production of ethanol and acetate. The maximum ethanol yield (0.652 mol mol−1 xylose) was obtained in the + 0.842 V reactor. The maximum acetate concentration (1,874 µmol L−1) was observed in the + 0.842 V reactor. The optimal potential for ethanol production was + 0.842 V with the maximum ethanol yield and energy saving. The application of positive potential caused the microorganisms to carry out ethanol fermentation, and the application of negative potential forced the microorganisms to carry out acetic fermentation. The potential application changed the diversity and community structure of microorganisms in the reactors, and the two most significantly changed families were Paenibacillaceae and Bacillaceae. Conclusion The constructed microbial electrolysis cells with different potentials obtained better production yield and selectivity compared with the reactor without applied potential. Our work provides strategies for the subsequent fermentation processes with different needs.Lei ChenMingpeng WangZhaojie ZhangYujie FengBMCarticleXylose consumptionEthanol productionElectro-fermentationExtracellular electron transferMicrobial community structureFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Xylose consumption Ethanol production Electro-fermentation Extracellular electron transfer Microbial community structure Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Xylose consumption Ethanol production Electro-fermentation Extracellular electron transfer Microbial community structure Fuel TP315-360 Biotechnology TP248.13-248.65 Lei Chen Mingpeng Wang Zhaojie Zhang Yujie Feng Application of electric potential improves ethanol production from xylose by active sludge
description	Abstract Background Low-cost raw materials such as lignocellulosic materials have been utilized in second-generation ethanol production process. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial lignocellulosic biorefinery. Results By applying different constant potentials to different microbial electrolysis cells with xylose as the sole carbon source, we analyzed the output of metabolites, microbial community structures, electron flow, and carbon flow in the process of xylose electro-fermentation by domesticated activated sludge. The bioreactors produced currents when applying positive potentials. The peak currents of the + 0.242 V, + 0.542 V and + 0.842 V reactors were 0.96 × 10–6 A, 3.36 × 10–6 A and 6.43 × 10–6 A, respectively. The application of potentials promoted the xylose consumption, and the maximum consumption rate in the + 0.542 V reactor was 95.5%, which was 34.8 times that of the reactor without applied potential. The potential application also promoted the production of ethanol and acetate. The maximum ethanol yield (0.652 mol mol−1 xylose) was obtained in the + 0.842 V reactor. The maximum acetate concentration (1,874 µmol L−1) was observed in the + 0.842 V reactor. The optimal potential for ethanol production was + 0.842 V with the maximum ethanol yield and energy saving. The application of positive potential caused the microorganisms to carry out ethanol fermentation, and the application of negative potential forced the microorganisms to carry out acetic fermentation. The potential application changed the diversity and community structure of microorganisms in the reactors, and the two most significantly changed families were Paenibacillaceae and Bacillaceae. Conclusion The constructed microbial electrolysis cells with different potentials obtained better production yield and selectivity compared with the reactor without applied potential. Our work provides strategies for the subsequent fermentation processes with different needs.
format	article
author	Lei Chen Mingpeng Wang Zhaojie Zhang Yujie Feng
author_facet	Lei Chen Mingpeng Wang Zhaojie Zhang Yujie Feng
author_sort	Lei Chen
title	Application of electric potential improves ethanol production from xylose by active sludge
title_short	Application of electric potential improves ethanol production from xylose by active sludge
title_full	Application of electric potential improves ethanol production from xylose by active sludge
title_fullStr	Application of electric potential improves ethanol production from xylose by active sludge
title_full_unstemmed	Application of electric potential improves ethanol production from xylose by active sludge
title_sort	application of electric potential improves ethanol production from xylose by active sludge
publisher	BMC
publishDate	2021
url	https://doaj.org/article/657db429a2a64b34812e0df71eb09f6b
work_keys_str_mv	AT leichen applicationofelectricpotentialimprovesethanolproductionfromxylosebyactivesludge AT mingpengwang applicationofelectricpotentialimprovesethanolproductionfromxylosebyactivesludge AT zhaojiezhang applicationofelectricpotentialimprovesethanolproductionfromxylosebyactivesludge AT yujiefeng applicationofelectricpotentialimprovesethanolproductionfromxylosebyactivesludge
_version_	1718419010414968832

Application of electric potential improves ethanol production from xylose by active sludge

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