Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants

Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinf...

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Autores principales:	Martin Hufbauer, Ulrike Wieland, Jürgen Gebel, Jochen Steinmann, Baki Akgül, Maren Eggers
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	human papillomavirus (HPV) HPV16 pseudovirus simian virus 40 (SV40) disinfection quantitative suspension test Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/658fcdda104b4523a48b123aa0093fb8
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spelling	oai:doaj.org-article:658fcdda104b4523a48b123aa0093fb82021-11-25T19:13:20ZInactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants10.3390/v131122071999-4915https://doaj.org/article/658fcdda104b4523a48b123aa0093fb82021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2207https://doaj.org/toc/1999-4915Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinfected gynecological equipment is a further potential transmission route. Since HPV cannot be easily grown in cell culture, polyomavirus SV40 has been used as a surrogate virus when testing the virucidal activity of chemical disinfectants. So far, studies that have compared the virucidal activity of different disinfectants against HPV and SV40 are lacking. Here, we evaluated the susceptibility of HPV16 pseudovirus and SV40 to seven active biocidal substances using quantitative suspension tests. Ethanol, glutaraldehyde (GTA), dodecyldipropylentriamin (DPTA), and ortho-phthalaldehydes (OPA) were able to reduce the infectivity of HPV16 pseudovirus >99.99% after 5 min. In contrast, isopropanol, peracetic acid (PAA), and quaternary ammonium compounds with alkylamines (QAC) only led to a slight or no reduction in infectivity. Concerning SV40, only GTA (60 min contact time), PAA, and OPA had virus-inactivating effects. In conclusion, the virucidal activity of three out of seven disinfectants tested was different for HPV16 pseudovirus and SV40. In this study, SV40 was shown to be a reliable surrogate virus for HPV when testing isopropanol-, GTA-, QAC-, and OPA-based disinfectants.Martin HufbauerUlrike WielandJürgen GebelJochen SteinmannBaki AkgülMaren EggersMDPI AGarticlehuman papillomavirus (HPV)HPV16 pseudovirussimian virus 40 (SV40)disinfectionquantitative suspension testMicrobiologyQR1-502ENViruses, Vol 13, Iss 2207, p 2207 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human papillomavirus (HPV) HPV16 pseudovirus simian virus 40 (SV40) disinfection quantitative suspension test Microbiology QR1-502
spellingShingle	human papillomavirus (HPV) HPV16 pseudovirus simian virus 40 (SV40) disinfection quantitative suspension test Microbiology QR1-502 Martin Hufbauer Ulrike Wieland Jürgen Gebel Jochen Steinmann Baki Akgül Maren Eggers Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
description	Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinfected gynecological equipment is a further potential transmission route. Since HPV cannot be easily grown in cell culture, polyomavirus SV40 has been used as a surrogate virus when testing the virucidal activity of chemical disinfectants. So far, studies that have compared the virucidal activity of different disinfectants against HPV and SV40 are lacking. Here, we evaluated the susceptibility of HPV16 pseudovirus and SV40 to seven active biocidal substances using quantitative suspension tests. Ethanol, glutaraldehyde (GTA), dodecyldipropylentriamin (DPTA), and ortho-phthalaldehydes (OPA) were able to reduce the infectivity of HPV16 pseudovirus >99.99% after 5 min. In contrast, isopropanol, peracetic acid (PAA), and quaternary ammonium compounds with alkylamines (QAC) only led to a slight or no reduction in infectivity. Concerning SV40, only GTA (60 min contact time), PAA, and OPA had virus-inactivating effects. In conclusion, the virucidal activity of three out of seven disinfectants tested was different for HPV16 pseudovirus and SV40. In this study, SV40 was shown to be a reliable surrogate virus for HPV when testing isopropanol-, GTA-, QAC-, and OPA-based disinfectants.
format	article
author	Martin Hufbauer Ulrike Wieland Jürgen Gebel Jochen Steinmann Baki Akgül Maren Eggers
author_facet	Martin Hufbauer Ulrike Wieland Jürgen Gebel Jochen Steinmann Baki Akgül Maren Eggers
author_sort	Martin Hufbauer
title	Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
title_short	Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
title_full	Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
title_fullStr	Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
title_full_unstemmed	Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants
title_sort	inactivation of polyomavirus sv40 as surrogate for human papillomaviruses by chemical disinfectants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/658fcdda104b4523a48b123aa0093fb8
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Inactivation of Polyomavirus SV40 as Surrogate for Human Papillomaviruses by Chemical Disinfectants

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