Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice

<i>Acinetobacter baumannii</i> has recently been increasing as an aggressive pathogen in immunocompromised persons. In the present study, we determined the in vitro antibacterial and anti-biofilm activity of thymoquinone (TQ) against <i>A. baumannii</i>. A liposomal formulati...

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Autores principales:	Khaled S. Allemailem, Ahmad Almatroudi, Faris Alrumaihi, Aseel Aljaghwani, Abdullah M. Alnuqaydan, Habibullah Khalilullah, Hina Younus, Asmaa M. El-Kady, Fahad M. Aldakheel, Amjad Ali Khan, Arif Khan, Masood Alam Khan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Acinetobacter baumannii</i> liposomes thymoquinone pneumonia immunocompromised mice Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6592ae0c5d1a4941ac671c5776f09121
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spelling	oai:doaj.org-article:6592ae0c5d1a4941ac671c5776f091212021-11-25T16:50:37ZAntimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice10.3390/biomedicines91116732227-9059https://doaj.org/article/6592ae0c5d1a4941ac671c5776f091212021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1673https://doaj.org/toc/2227-9059<i>Acinetobacter baumannii</i> has recently been increasing as an aggressive pathogen in immunocompromised persons. In the present study, we determined the in vitro antibacterial and anti-biofilm activity of thymoquinone (TQ) against <i>A. baumannii</i>. A liposomal formulation of TQ (Lip-TQ) was prepared and its therapeutic potential was investigated in the treatment of <i>A. baumannii</i> infection in immunocompromised mice. Leukopenia was induced in mice by injecting cyclophosphamide (CYP) at a dose of 200 mg/kg and the leukopenic mice were infected with 1 × 10<sup>6</sup> CFUs of <i>A. baumannii</i>. The effectiveness of free TQ or Lip-TQ against <i>A. baumannii</i> infection was assessed by analyzing the survival rate and bacterial burden. Moreover, the efficacy of Lip-TQ was also studied by examining the systemic inflammatory markers and the histological changes in the lung tissues. The results showed that the mice in the group treated with Lip-TQ at a dose of 10 mg/kg exhibited a 60% survival rate on day 40 post-infection, whereas all the mice treated with free TQ at the same dose died within this duration. Likewise, the lowest bacterial burden was found in the lung tissue of mice treated with Lip-TQ (10 mg/kg). Besides, Lip-TQ treatment remarkably alleviated the infection-associated inflammation, oxidative stress, and histological changes in the lung tissues. Based on the findings of the present study, we recommend considering Lip-TQ as a valuable therapeutic formulation in the treatment of <i>A. baumannii</i>-associated pneumonia in immunocompromised subjects.Khaled S. AllemailemAhmad AlmatroudiFaris AlrumaihiAseel AljaghwaniAbdullah M. AlnuqaydanHabibullah KhalilullahHina YounusAsmaa M. El-KadyFahad M. AldakheelAmjad Ali KhanArif KhanMasood Alam KhanMDPI AGarticle<i>Acinetobacter baumannii</i>liposomesthymoquinonepneumoniaimmunocompromised miceBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1673, p 1673 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Acinetobacter baumannii</i> liposomes thymoquinone pneumonia immunocompromised mice Biology (General) QH301-705.5
spellingShingle	<i>Acinetobacter baumannii</i> liposomes thymoquinone pneumonia immunocompromised mice Biology (General) QH301-705.5 Khaled S. Allemailem Ahmad Almatroudi Faris Alrumaihi Aseel Aljaghwani Abdullah M. Alnuqaydan Habibullah Khalilullah Hina Younus Asmaa M. El-Kady Fahad M. Aldakheel Amjad Ali Khan Arif Khan Masood Alam Khan Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
description	<i>Acinetobacter baumannii</i> has recently been increasing as an aggressive pathogen in immunocompromised persons. In the present study, we determined the in vitro antibacterial and anti-biofilm activity of thymoquinone (TQ) against <i>A. baumannii</i>. A liposomal formulation of TQ (Lip-TQ) was prepared and its therapeutic potential was investigated in the treatment of <i>A. baumannii</i> infection in immunocompromised mice. Leukopenia was induced in mice by injecting cyclophosphamide (CYP) at a dose of 200 mg/kg and the leukopenic mice were infected with 1 × 10<sup>6</sup> CFUs of <i>A. baumannii</i>. The effectiveness of free TQ or Lip-TQ against <i>A. baumannii</i> infection was assessed by analyzing the survival rate and bacterial burden. Moreover, the efficacy of Lip-TQ was also studied by examining the systemic inflammatory markers and the histological changes in the lung tissues. The results showed that the mice in the group treated with Lip-TQ at a dose of 10 mg/kg exhibited a 60% survival rate on day 40 post-infection, whereas all the mice treated with free TQ at the same dose died within this duration. Likewise, the lowest bacterial burden was found in the lung tissue of mice treated with Lip-TQ (10 mg/kg). Besides, Lip-TQ treatment remarkably alleviated the infection-associated inflammation, oxidative stress, and histological changes in the lung tissues. Based on the findings of the present study, we recommend considering Lip-TQ as a valuable therapeutic formulation in the treatment of <i>A. baumannii</i>-associated pneumonia in immunocompromised subjects.
format	article
author	Khaled S. Allemailem Ahmad Almatroudi Faris Alrumaihi Aseel Aljaghwani Abdullah M. Alnuqaydan Habibullah Khalilullah Hina Younus Asmaa M. El-Kady Fahad M. Aldakheel Amjad Ali Khan Arif Khan Masood Alam Khan
author_facet	Khaled S. Allemailem Ahmad Almatroudi Faris Alrumaihi Aseel Aljaghwani Abdullah M. Alnuqaydan Habibullah Khalilullah Hina Younus Asmaa M. El-Kady Fahad M. Aldakheel Amjad Ali Khan Arif Khan Masood Alam Khan
author_sort	Khaled S. Allemailem
title	Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
title_short	Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
title_full	Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
title_fullStr	Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
title_full_unstemmed	Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice
title_sort	antimicrobial, immunomodulatory and anti-inflammatory potential of liposomal thymoquinone: implications in the treatment of bacterial pneumonia in immunocompromised mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6592ae0c5d1a4941ac671c5776f09121
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Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice

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