In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.

It has been recently shown that N-ras plays a preferential role in immune cell development and function; specifically: N-ras, but not H-ras or K-ras, could be activated at and signal from the Golgi membrane of immune cells following a low level T-cell receptor stimulus. The goal of our studies was t...

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Autores principales:	Stephen J Lynch, Jiri Zavadil, Angel Pellicer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65966cd457c5420a8dc30c5b6d3717cb
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spelling	oai:doaj.org-article:65966cd457c5420a8dc30c5b6d3717cb2021-11-18T07:43:18ZIn TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.1932-620310.1371/journal.pone.0063193https://doaj.org/article/65966cd457c5420a8dc30c5b6d3717cb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755101/?tool=EBIhttps://doaj.org/toc/1932-6203It has been recently shown that N-ras plays a preferential role in immune cell development and function; specifically: N-ras, but not H-ras or K-ras, could be activated at and signal from the Golgi membrane of immune cells following a low level T-cell receptor stimulus. The goal of our studies was to test the hypothesis that N-ras and H-ras played distinct roles in immune cells at the level of the transcriptome. First, we showed via mRNA expression profiling that there were over four hundred genes that were uniquely differentially regulated either by N-ras or H-ras, which provided strong evidence in favor of the hypothesis that N-ras and H-ras have distinct functions in immune cells. We next characterized the genes that were differentially regulated by N-ras in T cells following a low-level T-cell receptor stimulus. Of the large pool of candidate genes that were differentially regulated by N-ras downstream of TCR ligation, four genes were verified in qRT-PCR-based validation experiments (Dntt, Slc9a6, Chst1, and Lars2). Finally, although there was little overlap between individual genes that were regulated by N-ras in unstimulated thymocytes and stimulated CD4(+) T-cells, there was a nearly complete correspondence between the signaling pathways that were regulated by N-ras in these two immune cell types.Stephen J LynchJiri ZavadilAngel PellicerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e63193 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen J Lynch Jiri Zavadil Angel Pellicer In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
description	It has been recently shown that N-ras plays a preferential role in immune cell development and function; specifically: N-ras, but not H-ras or K-ras, could be activated at and signal from the Golgi membrane of immune cells following a low level T-cell receptor stimulus. The goal of our studies was to test the hypothesis that N-ras and H-ras played distinct roles in immune cells at the level of the transcriptome. First, we showed via mRNA expression profiling that there were over four hundred genes that were uniquely differentially regulated either by N-ras or H-ras, which provided strong evidence in favor of the hypothesis that N-ras and H-ras have distinct functions in immune cells. We next characterized the genes that were differentially regulated by N-ras in T cells following a low-level T-cell receptor stimulus. Of the large pool of candidate genes that were differentially regulated by N-ras downstream of TCR ligation, four genes were verified in qRT-PCR-based validation experiments (Dntt, Slc9a6, Chst1, and Lars2). Finally, although there was little overlap between individual genes that were regulated by N-ras in unstimulated thymocytes and stimulated CD4(+) T-cells, there was a nearly complete correspondence between the signaling pathways that were regulated by N-ras in these two immune cell types.
format	article
author	Stephen J Lynch Jiri Zavadil Angel Pellicer
author_facet	Stephen J Lynch Jiri Zavadil Angel Pellicer
author_sort	Stephen J Lynch
title	In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
title_short	In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
title_full	In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
title_fullStr	In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
title_full_unstemmed	In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.
title_sort	in tcr-stimulated t-cells, n-ras regulates specific genes and signal transduction pathways.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/65966cd457c5420a8dc30c5b6d3717cb
work_keys_str_mv	AT stephenjlynch intcrstimulatedtcellsnrasregulatesspecificgenesandsignaltransductionpathways AT jirizavadil intcrstimulatedtcellsnrasregulatesspecificgenesandsignaltransductionpathways AT angelpellicer intcrstimulatedtcellsnrasregulatesspecificgenesandsignaltransductionpathways
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In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.

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