Identification of novel microRNAs in the sheep heart and their regulation in heart failure

Abstract Study of microRNA (miRNAs) using sheep models is limited due to lack of miRNA information. We therefore investigated oar-miRNAs and their regulation in an ovine model of heart failure (HF). Left ventricular (LV) tissue was collected from normal (Cont), HF (LV pacing @ ~220bpm for 13-days) a...

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Autores principales:	Lee Lee Wong, Miriam T. Rademaker, Eng Leng Saw, Kar Sheng Lew, Leigh J. Ellmers, Christopher J. Charles, Arthur Mark Richards, Peipei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65989429538a47d9857a46a93f35e005
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spelling	oai:doaj.org-article:65989429538a47d9857a46a93f35e0052021-12-02T11:52:38ZIdentification of novel microRNAs in the sheep heart and their regulation in heart failure10.1038/s41598-017-08574-x2045-2322https://doaj.org/article/65989429538a47d9857a46a93f35e0052017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08574-xhttps://doaj.org/toc/2045-2322Abstract Study of microRNA (miRNAs) using sheep models is limited due to lack of miRNA information. We therefore investigated oar-miRNAs and their regulation in an ovine model of heart failure (HF). Left ventricular (LV) tissue was collected from normal (Cont), HF (LV pacing @ ~220bpm for 13-days) and HF-recovery sheep (HF-R, 26-days after pacing cessation). MiRNA expression was profiled using next-generation sequencing (NGS) and miRNA array, and validated by stem-loop qPCR. Detected sequences were mapped against the ovine genome (Oar v4.0) and aligned with known miRNAs (miRBase v21). A total of 36,438,340 raw reads were obtained with a peak distribution of 18–23 nt. Of these, 637 miRNAs were detected by NGS and mapped to the ovine genome. With cut-off at 10 counts, 275 novel miRNAs were identified (with 186 showing 100% alignment and 89 showing 70–99% alignment with human/mouse and/or rat miRNAs, respectively), and 78 known oar-miRNAs. Cardiac-enriched miRNA-1, -133a, -208a/b and -499 were highly expressed in the LV. With HF induction, miRNA-133b-3p, -208b-3p, -125a-5p, -125b-5p, -126-3p, -21-5p, -210-3p, -29a-3p, -320a and -494-3p were significantly up-regulated relative to Cont and tended to return to normal levels following HF-recovery. This study has expanded the sheep miRNA database, and demonstrated HF-induced regulation of miRNAs.Lee Lee WongMiriam T. RademakerEng Leng SawKar Sheng LewLeigh J. EllmersChristopher J. CharlesArthur Mark RichardsPeipei WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lee Lee Wong Miriam T. Rademaker Eng Leng Saw Kar Sheng Lew Leigh J. Ellmers Christopher J. Charles Arthur Mark Richards Peipei Wang Identification of novel microRNAs in the sheep heart and their regulation in heart failure
description	Abstract Study of microRNA (miRNAs) using sheep models is limited due to lack of miRNA information. We therefore investigated oar-miRNAs and their regulation in an ovine model of heart failure (HF). Left ventricular (LV) tissue was collected from normal (Cont), HF (LV pacing @ ~220bpm for 13-days) and HF-recovery sheep (HF-R, 26-days after pacing cessation). MiRNA expression was profiled using next-generation sequencing (NGS) and miRNA array, and validated by stem-loop qPCR. Detected sequences were mapped against the ovine genome (Oar v4.0) and aligned with known miRNAs (miRBase v21). A total of 36,438,340 raw reads were obtained with a peak distribution of 18–23 nt. Of these, 637 miRNAs were detected by NGS and mapped to the ovine genome. With cut-off at 10 counts, 275 novel miRNAs were identified (with 186 showing 100% alignment and 89 showing 70–99% alignment with human/mouse and/or rat miRNAs, respectively), and 78 known oar-miRNAs. Cardiac-enriched miRNA-1, -133a, -208a/b and -499 were highly expressed in the LV. With HF induction, miRNA-133b-3p, -208b-3p, -125a-5p, -125b-5p, -126-3p, -21-5p, -210-3p, -29a-3p, -320a and -494-3p were significantly up-regulated relative to Cont and tended to return to normal levels following HF-recovery. This study has expanded the sheep miRNA database, and demonstrated HF-induced regulation of miRNAs.
format	article
author	Lee Lee Wong Miriam T. Rademaker Eng Leng Saw Kar Sheng Lew Leigh J. Ellmers Christopher J. Charles Arthur Mark Richards Peipei Wang
author_facet	Lee Lee Wong Miriam T. Rademaker Eng Leng Saw Kar Sheng Lew Leigh J. Ellmers Christopher J. Charles Arthur Mark Richards Peipei Wang
author_sort	Lee Lee Wong
title	Identification of novel microRNAs in the sheep heart and their regulation in heart failure
title_short	Identification of novel microRNAs in the sheep heart and their regulation in heart failure
title_full	Identification of novel microRNAs in the sheep heart and their regulation in heart failure
title_fullStr	Identification of novel microRNAs in the sheep heart and their regulation in heart failure
title_full_unstemmed	Identification of novel microRNAs in the sheep heart and their regulation in heart failure
title_sort	identification of novel micrornas in the sheep heart and their regulation in heart failure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/65989429538a47d9857a46a93f35e005
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Identification of novel microRNAs in the sheep heart and their regulation in heart failure

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