Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Cong Chen,1,* Feng Jia,2,* Zhibo Hou,3 Shu Ruan,4 Qibin Lu1 1Department of Gynecology of Traditional Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 2Department of Neurosurgery, Yancheng City No 1 P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen C, Jia F, Hou Z, Ruan S, Lu Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Nanoparticles Drug delivery Paeonol Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/659b12a81f184de68164db564784b933
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:659b12a81f184de68164db564784b933
record_format	dspace
spelling	oai:doaj.org-article:659b12a81f184de68164db564784b9332021-12-02T02:41:16ZDelivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects1178-2013https://doaj.org/article/659b12a81f184de68164db564784b9332017-09-01T00:00:00Zhttps://www.dovepress.com/delivery-of-paeonol-by-nanoparticles-enhances-its-in-vitro-and-in-vivo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cong Chen,1,* Feng Jia,2,* Zhibo Hou,3 Shu Ruan,4 Qibin Lu1 1Department of Gynecology of Traditional Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 2Department of Neurosurgery, Yancheng City No 1 People’s Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, 3First Department of Respiratory Medicine, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, 4Department of Endocrinology, Yancheng Third Hospital, The Affiliated Hospital of Southeast University Medical College, Yancheng, Jiangsu, China *These authors contributed equally to this work Abstract: Paeonol (Pae; 2'-hydroxy-4'-methoxyacetophenone) has attracted intense attention as a potential therapeutic agent against various cancers. However, the use of Pae is limited owing to its hydrophobicity. Recently, biodegradable polymeric nanoparticles with amphiphilic copolymers have been used as drug carriers; these have better bioavailability and are promising tumor-targeted drug delivery systems. In the current study, we prepared Pae-loaded nanoparticles (Pae-NPs) with amphiphilic block copolymers using nanoprecipitation. The physiochemical characteristics and antitumor effects of nanoparticles were evaluated in different cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed substantial inhibition of cell growth by Pae-NPs. Moreover, lower doses of Pae-NPs inhibited cell growth more efficiently than the equivalent doses of free Pae. Inhibition was characterized by significant elevation of intracellular reactive oxygen species and subsequent inhibition of Akt and regulation of apoptotic proteins, which could be partly reversed by pretreatment with the antioxidant N-acetylcysteine. In vivo results also demonstrated that Pae-NPs could exert much stronger antitumor effects than free Pae. Therefore, Pae-NPs represent a promising delivery system to overcome the low solubility of Pae and enable its use in treating cancer. Keywords: nanoparticles, drug delivery, paeonolChen CJia FHou ZRuan SLu QDove Medical PressarticleNanoparticlesDrug deliveryPaeonolMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6605-6616 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoparticles Drug delivery Paeonol Medicine (General) R5-920
spellingShingle	Nanoparticles Drug delivery Paeonol Medicine (General) R5-920 Chen C Jia F Hou Z Ruan S Lu Q Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
description	Cong Chen,1,* Feng Jia,2,* Zhibo Hou,3 Shu Ruan,4 Qibin Lu1 1Department of Gynecology of Traditional Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 2Department of Neurosurgery, Yancheng City No 1 People’s Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, 3First Department of Respiratory Medicine, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, 4Department of Endocrinology, Yancheng Third Hospital, The Affiliated Hospital of Southeast University Medical College, Yancheng, Jiangsu, China *These authors contributed equally to this work Abstract: Paeonol (Pae; 2'-hydroxy-4'-methoxyacetophenone) has attracted intense attention as a potential therapeutic agent against various cancers. However, the use of Pae is limited owing to its hydrophobicity. Recently, biodegradable polymeric nanoparticles with amphiphilic copolymers have been used as drug carriers; these have better bioavailability and are promising tumor-targeted drug delivery systems. In the current study, we prepared Pae-loaded nanoparticles (Pae-NPs) with amphiphilic block copolymers using nanoprecipitation. The physiochemical characteristics and antitumor effects of nanoparticles were evaluated in different cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed substantial inhibition of cell growth by Pae-NPs. Moreover, lower doses of Pae-NPs inhibited cell growth more efficiently than the equivalent doses of free Pae. Inhibition was characterized by significant elevation of intracellular reactive oxygen species and subsequent inhibition of Akt and regulation of apoptotic proteins, which could be partly reversed by pretreatment with the antioxidant N-acetylcysteine. In vivo results also demonstrated that Pae-NPs could exert much stronger antitumor effects than free Pae. Therefore, Pae-NPs represent a promising delivery system to overcome the low solubility of Pae and enable its use in treating cancer. Keywords: nanoparticles, drug delivery, paeonol
format	article
author	Chen C Jia F Hou Z Ruan S Lu Q
author_facet	Chen C Jia F Hou Z Ruan S Lu Q
author_sort	Chen C
title	Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
title_short	Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
title_full	Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
title_fullStr	Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
title_full_unstemmed	Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
title_sort	delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/659b12a81f184de68164db564784b933
work_keys_str_mv	AT chenc deliveryofpaeonolbynanoparticlesenhancesitsinvitroandinvivoantitumoreffects AT jiaf deliveryofpaeonolbynanoparticlesenhancesitsinvitroandinvivoantitumoreffects AT houz deliveryofpaeonolbynanoparticlesenhancesitsinvitroandinvivoantitumoreffects AT ruans deliveryofpaeonolbynanoparticlesenhancesitsinvitroandinvivoantitumoreffects AT luq deliveryofpaeonolbynanoparticlesenhancesitsinvitroandinvivoantitumoreffects
_version_	1718402290401935360

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Ejemplares similares