Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.

Recent studies using culture-independent methods have characterized the human airway microbiota and report microbial communities distinct from other body sites. Changes in these airway bacterial communities appear to be associated with inflammatory lung disease, yet the pro-inflammatory properties o...

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Autores principales:	Jeppe Madura Larsen, Daniel Bisgaard Steen-Jensen, Janne Marie Laursen, Jonas Nørskov Søndergaard, Hanieh Sadat Musavian, Tariq Mahmood Butt, Susanne Brix
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65a2169ee5674451902e4aaa0c92da4f
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spelling	oai:doaj.org-article:65a2169ee5674451902e4aaa0c92da4f2021-11-18T07:27:13ZDivergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.1932-620310.1371/journal.pone.0031976https://doaj.org/article/65a2169ee5674451902e4aaa0c92da4f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363778/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies using culture-independent methods have characterized the human airway microbiota and report microbial communities distinct from other body sites. Changes in these airway bacterial communities appear to be associated with inflammatory lung disease, yet the pro-inflammatory properties of individual bacterial species are unknown. In this study, we compared the immune stimulatory capacity on human monocyte-derived dendritic cells (DCs) of selected airway commensal and pathogenic bacteria predominantly associated with lungs of asthma or COPD patients (pathogenic Haemophillus spp. and Moraxella spp.), healthy lungs (commensal Prevotella spp.) or both (commensal Veillonella spp. and Actinomyces spp.). All bacteria were found to induce activation of DCs as demonstrated by similar induction of CD83, CD40 and CD86 surface expression. However, asthma and COPD-associated pathogenic bacteria provoked a 3-5 fold higher production of IL-23, IL-12p70 and IL-10 cytokines compared to the commensal bacteria. Based on the differential cytokine production profiles, the studied airway bacteria could be segregated into three groups (Haemophilus spp. and Moraxella spp. vs. Prevotella spp. and Veillonella spp. vs. Actinomyces spp.) reflecting their pro-inflammatory effects on DCs. Co-culture experiments found that Prevotella spp. were able to reduce Haemophillus influenzae-induced IL-12p70 in DCs, whereas no effect was observed on IL-23 and IL-10 production. This study demonstrates intrinsic differences in DC stimulating properties of bacteria associated with the airway microbiota.Jeppe Madura LarsenDaniel Bisgaard Steen-JensenJanne Marie LaursenJonas Nørskov SøndergaardHanieh Sadat MusavianTariq Mahmood ButtSusanne BrixPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31976 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeppe Madura Larsen Daniel Bisgaard Steen-Jensen Janne Marie Laursen Jonas Nørskov Søndergaard Hanieh Sadat Musavian Tariq Mahmood Butt Susanne Brix Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
description	Recent studies using culture-independent methods have characterized the human airway microbiota and report microbial communities distinct from other body sites. Changes in these airway bacterial communities appear to be associated with inflammatory lung disease, yet the pro-inflammatory properties of individual bacterial species are unknown. In this study, we compared the immune stimulatory capacity on human monocyte-derived dendritic cells (DCs) of selected airway commensal and pathogenic bacteria predominantly associated with lungs of asthma or COPD patients (pathogenic Haemophillus spp. and Moraxella spp.), healthy lungs (commensal Prevotella spp.) or both (commensal Veillonella spp. and Actinomyces spp.). All bacteria were found to induce activation of DCs as demonstrated by similar induction of CD83, CD40 and CD86 surface expression. However, asthma and COPD-associated pathogenic bacteria provoked a 3-5 fold higher production of IL-23, IL-12p70 and IL-10 cytokines compared to the commensal bacteria. Based on the differential cytokine production profiles, the studied airway bacteria could be segregated into three groups (Haemophilus spp. and Moraxella spp. vs. Prevotella spp. and Veillonella spp. vs. Actinomyces spp.) reflecting their pro-inflammatory effects on DCs. Co-culture experiments found that Prevotella spp. were able to reduce Haemophillus influenzae-induced IL-12p70 in DCs, whereas no effect was observed on IL-23 and IL-10 production. This study demonstrates intrinsic differences in DC stimulating properties of bacteria associated with the airway microbiota.
format	article
author	Jeppe Madura Larsen Daniel Bisgaard Steen-Jensen Janne Marie Laursen Jonas Nørskov Søndergaard Hanieh Sadat Musavian Tariq Mahmood Butt Susanne Brix
author_facet	Jeppe Madura Larsen Daniel Bisgaard Steen-Jensen Janne Marie Laursen Jonas Nørskov Søndergaard Hanieh Sadat Musavian Tariq Mahmood Butt Susanne Brix
author_sort	Jeppe Madura Larsen
title	Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
title_short	Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
title_full	Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
title_fullStr	Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
title_full_unstemmed	Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
title_sort	divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/65a2169ee5674451902e4aaa0c92da4f
work_keys_str_mv	AT jeppemaduralarsen divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT danielbisgaardsteenjensen divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT jannemarielaursen divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT jonasnørskovsøndergaard divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT haniehsadatmusavian divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT tariqmahmoodbutt divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota AT susannebrix divergentproinflammatoryprofileofhumandendriticcellsinresponsetocommensalandpathogenicbacteriaassociatedwiththeairwaymicrobiota
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Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota.

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