Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.

<h4>Background</h4>Although the study of respiratory microbiota has been an active field of research, obtaining the appropriate respiratory samples for healthy controls remains to be a challenge. As such, this study aims to evaluate the use of endotracheal tube washing as a viable contro...

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Autores principales:	Sun Young Cho, Jeong-Hyun Choi, Seung Hyeun Lee, Yong-Sung Choi, Sung Wook Hwang, Young Jin Kim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65a31b172ef94ae088386caf86df6afb
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spelling	oai:doaj.org-article:65a31b172ef94ae088386caf86df6afb2021-12-02T20:06:02ZMetataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.1932-620310.1371/journal.pone.0259596https://doaj.org/article/65a31b172ef94ae088386caf86df6afb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259596https://doaj.org/toc/1932-6203<h4>Background</h4>Although the study of respiratory microbiota has been an active field of research, obtaining the appropriate respiratory samples for healthy controls remains to be a challenge. As such, this study aims to evaluate the use of endotracheal tube washing as a viable control for sputum samples.<h4>Methods</h4>A total of 14 subjects, including 8 healthy respiratory controls and 6 diabetic patients without any respiratory disease, were enrolled in this study, during which the endotracheal tubes used in their scheduled routine surgery were collected. Pre-operative oral gargles were also collected from non-diabetic subjects.<h4>Results</h4>16S amplicon sequencing revealed similar taxa composition in endotracheal tube washings and oral gargles in the healthy control subjects, although the relative abundance of 11 genus level operational taxonomic units was significantly different between the two sample sources. The diabetic subjects showed relatively lower diversity than those of non-diabetic subjects. The proportion range of the most abundant taxa detected in each endotracheal tube washings were 10.1-33.2%.<h4>Conclusion</h4>Endotracheal tube washing fluid may provide healthy control samples for upper respiratory investigations without incurring any additional risk to the subject.Sun Young ChoJeong-Hyun ChoiSeung Hyeun LeeYong-Sung ChoiSung Wook HwangYoung Jin KimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259596 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sun Young Cho Jeong-Hyun Choi Seung Hyeun Lee Yong-Sung Choi Sung Wook Hwang Young Jin Kim Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
description	<h4>Background</h4>Although the study of respiratory microbiota has been an active field of research, obtaining the appropriate respiratory samples for healthy controls remains to be a challenge. As such, this study aims to evaluate the use of endotracheal tube washing as a viable control for sputum samples.<h4>Methods</h4>A total of 14 subjects, including 8 healthy respiratory controls and 6 diabetic patients without any respiratory disease, were enrolled in this study, during which the endotracheal tubes used in their scheduled routine surgery were collected. Pre-operative oral gargles were also collected from non-diabetic subjects.<h4>Results</h4>16S amplicon sequencing revealed similar taxa composition in endotracheal tube washings and oral gargles in the healthy control subjects, although the relative abundance of 11 genus level operational taxonomic units was significantly different between the two sample sources. The diabetic subjects showed relatively lower diversity than those of non-diabetic subjects. The proportion range of the most abundant taxa detected in each endotracheal tube washings were 10.1-33.2%.<h4>Conclusion</h4>Endotracheal tube washing fluid may provide healthy control samples for upper respiratory investigations without incurring any additional risk to the subject.
format	article
author	Sun Young Cho Jeong-Hyun Choi Seung Hyeun Lee Yong-Sung Choi Sung Wook Hwang Young Jin Kim
author_facet	Sun Young Cho Jeong-Hyun Choi Seung Hyeun Lee Yong-Sung Choi Sung Wook Hwang Young Jin Kim
author_sort	Sun Young Cho
title	Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
title_short	Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
title_full	Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
title_fullStr	Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
title_full_unstemmed	Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
title_sort	metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/65a31b172ef94ae088386caf86df6afb
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Metataxonomic investigation of the microbial community in the trachea and oropharynx of healthy controls and diabetic patients using endotracheal tubes.

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