Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles

Chean Yeah Yong,1 Swee Keong Yeap,2 Kok Lian Ho,3 Abdul Rahman Omar,2,4 Wen Siang Tan1,2 1Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, 2Institute of Bioscience, 3Department of Pathology, Faculty of Medicine and Health Sciences, 4Department of Veterinary Pathology...

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Autores principales:	Yong CY, Yeap SK, Ho KL, Omar AR, Tan WS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65a597deb19c4e738defa723e564fcd9
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spelling	oai:doaj.org-article:65a597deb19c4e738defa723e564fcd92021-12-02T00:46:38ZPotential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles1178-2013https://doaj.org/article/65a597deb19c4e738defa723e564fcd92015-04-01T00:00:00Zhttp://www.dovepress.com/potential-recombinant-vaccine-against-influenza-a-virus-based-on-m2e-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Chean Yeah Yong,1 Swee Keong Yeap,2 Kok Lian Ho,3 Abdul Rahman Omar,2,4 Wen Siang Tan1,2 1Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, 2Institute of Bioscience, 3Department of Pathology, Faculty of Medicine and Health Sciences, 4Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia Abstract: Influenza A virus poses a major threat to human health, causing outbreaks from time to time. Currently available vaccines employ inactivated viruses of different strains to provide protection against influenza virus infection. However, high mutation rates of influenza virus hemagglutinin (H) and neuraminidase (N) glycoproteins give rise to vaccine escape mutants. Thus, an effective vaccine providing protection against all strains of influenza virus would be a valuable asset. The ectodomain of matrix 2 protein (M2e) was found to be highly conserved despite mutations of the H and N glycoproteins. Hence, one to five copies of M2e were fused to the carboxyl-terminal end of the recombinant nodavirus capsid protein derived from Macrobrachium rosenbergii. The chimeric proteins harboring up to five copies of M2e formed nanosized virus-like particles approximately 30 nm in diameter, which could be purified easily by immobilized metal affinity chromatography. BALB/c mice immunized subcutaneously with these chimeric proteins developed antibodies specifically against M2e, and the titer was proportional to the copy numbers of M2e displayed on the nodavirus capsid nanoparticles. The fusion proteins also induced a type 1 T helper immune response. Collectively, M2e displayed on the nodavirus capsid nanoparticles could provide an alternative solution to a possible influenza pandemic in the future. Keywords: matrix 2 ectodomain, nodavirus capsid, virus-like particle, fusion protein, subunit vaccine, immunogenicityYong CYYeap SKHo KLOmar ARTan WSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2751-2763 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yong CY Yeap SK Ho KL Omar AR Tan WS Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
description	Chean Yeah Yong,1 Swee Keong Yeap,2 Kok Lian Ho,3 Abdul Rahman Omar,2,4 Wen Siang Tan1,2 1Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, 2Institute of Bioscience, 3Department of Pathology, Faculty of Medicine and Health Sciences, 4Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia Abstract: Influenza A virus poses a major threat to human health, causing outbreaks from time to time. Currently available vaccines employ inactivated viruses of different strains to provide protection against influenza virus infection. However, high mutation rates of influenza virus hemagglutinin (H) and neuraminidase (N) glycoproteins give rise to vaccine escape mutants. Thus, an effective vaccine providing protection against all strains of influenza virus would be a valuable asset. The ectodomain of matrix 2 protein (M2e) was found to be highly conserved despite mutations of the H and N glycoproteins. Hence, one to five copies of M2e were fused to the carboxyl-terminal end of the recombinant nodavirus capsid protein derived from Macrobrachium rosenbergii. The chimeric proteins harboring up to five copies of M2e formed nanosized virus-like particles approximately 30 nm in diameter, which could be purified easily by immobilized metal affinity chromatography. BALB/c mice immunized subcutaneously with these chimeric proteins developed antibodies specifically against M2e, and the titer was proportional to the copy numbers of M2e displayed on the nodavirus capsid nanoparticles. The fusion proteins also induced a type 1 T helper immune response. Collectively, M2e displayed on the nodavirus capsid nanoparticles could provide an alternative solution to a possible influenza pandemic in the future. Keywords: matrix 2 ectodomain, nodavirus capsid, virus-like particle, fusion protein, subunit vaccine, immunogenicity
format	article
author	Yong CY Yeap SK Ho KL Omar AR Tan WS
author_facet	Yong CY Yeap SK Ho KL Omar AR Tan WS
author_sort	Yong CY
title	Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
title_short	Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
title_full	Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
title_fullStr	Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
title_full_unstemmed	Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
title_sort	potential recombinant vaccine against influenza a virus based on m2e displayed on nodaviral capsid nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/65a597deb19c4e738defa723e564fcd9
work_keys_str_mv	AT yongcy potentialrecombinantvaccineagainstinfluenzaavirusbasedonm2edisplayedonnodaviralcapsidnanoparticles AT yeapsk potentialrecombinantvaccineagainstinfluenzaavirusbasedonm2edisplayedonnodaviralcapsidnanoparticles AT hokl potentialrecombinantvaccineagainstinfluenzaavirusbasedonm2edisplayedonnodaviralcapsidnanoparticles AT omarar potentialrecombinantvaccineagainstinfluenzaavirusbasedonm2edisplayedonnodaviralcapsidnanoparticles AT tanws potentialrecombinantvaccineagainstinfluenzaavirusbasedonm2edisplayedonnodaviralcapsidnanoparticles
_version_	1718403500356927488

Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles

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