Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positi...

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Autores principales:	Seyda Cengiz, Gökçen Dinç, Ünlü Söğüt Mehtap
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	escherichia coli virulence properties antibiotic resistance phylogeneticrelationship cow mastitis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/65a6a097985743ea80c60db2448a8b42
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spelling	oai:doaj.org-article:65a6a097985743ea80c60db2448a8b422021-11-17T21:27:52ZDetection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis1820-744810.2478/acve-2014-0039https://doaj.org/article/65a6a097985743ea80c60db2448a8b422014-12-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0039https://doaj.org/toc/1820-7448Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofilm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no significant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In field conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profiles in herds should be monitored and effective antibiotics should be administeredSeyda CengizGökçen DinçÜnlü Söğüt MehtapSciendoarticleescherichia colivirulence propertiesantibiotic resistancephylogeneticrelationshipcow mastitisVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 4, Pp 413-425 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	escherichia coli virulence properties antibiotic resistance phylogeneticrelationship cow mastitis Veterinary medicine SF600-1100
spellingShingle	escherichia coli virulence properties antibiotic resistance phylogeneticrelationship cow mastitis Veterinary medicine SF600-1100 Seyda Cengiz Gökçen Dinç Ünlü Söğüt Mehtap Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
description	Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofilm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no significant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In field conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profiles in herds should be monitored and effective antibiotics should be administered
format	article
author	Seyda Cengiz Gökçen Dinç Ünlü Söğüt Mehtap
author_facet	Seyda Cengiz Gökçen Dinç Ünlü Söğüt Mehtap
author_sort	Seyda Cengiz
title	Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
title_short	Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
title_full	Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
title_fullStr	Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
title_full_unstemmed	Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis
title_sort	detection of several virulence properties, antibiotic resistance and phylogenetic relationship in e. coli isolates originated from cow mastitis
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/65a6a097985743ea80c60db2448a8b42
work_keys_str_mv	AT seydacengiz detectionofseveralvirulencepropertiesantibioticresistanceandphylogeneticrelationshipinecoliisolatesoriginatedfromcowmastitis AT gokcendinc detectionofseveralvirulencepropertiesantibioticresistanceandphylogeneticrelationshipinecoliisolatesoriginatedfromcowmastitis AT unlusogutmehtap detectionofseveralvirulencepropertiesantibioticresistanceandphylogeneticrelationshipinecoliisolatesoriginatedfromcowmastitis
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Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

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