Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising pr...

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Autores principales:	Maria Paola Tomasino, Mariana Aparício, Inês Ribeiro, Filipa Santos, Miguel Caetano, C. Marisa R. Almeida, Maria de Fátima Carvalho, Ana P. Mucha
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1
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spelling	oai:doaj.org-article:65b07f0aaf55470a9e56cb4e3c01e8b12021-11-25T18:25:38ZDiversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)10.3390/microorganisms91123892076-2607https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b12021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2389https://doaj.org/toc/2076-2607Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater.Maria Paola TomasinoMariana AparícioInês RibeiroFilipa SantosMiguel CaetanoC. Marisa R. AlmeidaMaria de Fátima CarvalhoAna P. MuchaMDPI AGarticlemicrobial consortiapetroleum hydrocarbonsbioremediationdeep-sea16S rRNA genenext-generation sequencingBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2389, p 2389 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5
spellingShingle	microbial consortia petroleum hydrocarbons bioremediation deep-sea 16S rRNA gene next-generation sequencing Biology (General) QH301-705.5 Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
description	Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater.
format	article
author	Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha
author_facet	Maria Paola Tomasino Mariana Aparício Inês Ribeiro Filipa Santos Miguel Caetano C. Marisa R. Almeida Maria de Fátima Carvalho Ana P. Mucha
author_sort	Maria Paola Tomasino
title	Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
title_short	Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
title_full	Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
title_fullStr	Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
title_full_unstemmed	Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)
title_sort	diversity and hydrocarbon-degrading potential of deep-sea microbial community from the mid-atlantic ridge, south of the azores (north atlantic ocean)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/65b07f0aaf55470a9e56cb4e3c01e8b1
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Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

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