Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking

Rinat Ankri,1 Susanne Melzer,2,3 Attila Tarnok,2,3 Dror Fixler1 1Faculty of Engineering, Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel; 2Research Department of Pediatric Cardiology, Heart Centre Leipzig GmbH, 3Translational Centre for Regenerative Medici...

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Autores principales:	Ankri R, Melzer S, Tarnok A, Fixler D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65bce4d04cbb492d93ebb032c17804dc
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spelling	oai:doaj.org-article:65bce4d04cbb492d93ebb032c17804dc2021-12-02T03:11:49ZDetection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking1178-2013https://doaj.org/article/65bce4d04cbb492d93ebb032c17804dc2015-07-01T00:00:00Zhttp://www.dovepress.com/detection-of-gold-nanorods-uptake-by-macrophages-using-scattering-anal-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rinat Ankri,1 Susanne Melzer,2,3 Attila Tarnok,2,3 Dror Fixler1 1Faculty of Engineering, Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel; 2Research Department of Pediatric Cardiology, Heart Centre Leipzig GmbH, 3Translational Centre for Regenerative Medicine (TRM) Leipzig, University of Leipzig, Leipzig, Germany Abstract: In this study, we report a potential noninvasive technique for the detection of vulnerable plaques using scatter analyses with flow cytometry (FCM) method combined with the diffusion reflection (DR) method. The atherosclerotic plaques are commonly divided into two major categories: stable and vulnerable. The vulnerable plaques are rich with inflammatory cells, mostly macrophages (MФ), which release enzymes that break down collagen in the cap. The detection method is based on uptake of gold nanorods (GNR) by MФ. The GNR have unique optical properties that enable their detection using the FCM method, based on their scattering properties, and using the DR method, based on their unique absorption properties. This work demonstrates that after GNR labeling of MФ, 1) the FCM scatter values increased up to 3.7-fold with arbitrary intensity values increasing from 1,110 to 4,100 and 2) the DR slope changed from an average slope of 0.196 (MФ only) to an average slope of 0.827 (MФ labeled with GNR) (P<0.001 for both cases). The combination of FCM and DR measurements provides a potential novel, highly sensitive, and noninvasive method for the identification of atherosclerotic vulnerable plaques, aimed to develop a potential tool for in vivo tracking. Keywords: gold nanoparticles, macrophages, noninvasive detection, flow cytometry, vulnerable plaquesAnkri RMelzer STarnok AFixler DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4437-4446 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ankri R Melzer S Tarnok A Fixler D Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
description	Rinat Ankri,1 Susanne Melzer,2,3 Attila Tarnok,2,3 Dror Fixler1 1Faculty of Engineering, Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel; 2Research Department of Pediatric Cardiology, Heart Centre Leipzig GmbH, 3Translational Centre for Regenerative Medicine (TRM) Leipzig, University of Leipzig, Leipzig, Germany Abstract: In this study, we report a potential noninvasive technique for the detection of vulnerable plaques using scatter analyses with flow cytometry (FCM) method combined with the diffusion reflection (DR) method. The atherosclerotic plaques are commonly divided into two major categories: stable and vulnerable. The vulnerable plaques are rich with inflammatory cells, mostly macrophages (MФ), which release enzymes that break down collagen in the cap. The detection method is based on uptake of gold nanorods (GNR) by MФ. The GNR have unique optical properties that enable their detection using the FCM method, based on their scattering properties, and using the DR method, based on their unique absorption properties. This work demonstrates that after GNR labeling of MФ, 1) the FCM scatter values increased up to 3.7-fold with arbitrary intensity values increasing from 1,110 to 4,100 and 2) the DR slope changed from an average slope of 0.196 (MФ only) to an average slope of 0.827 (MФ labeled with GNR) (P<0.001 for both cases). The combination of FCM and DR measurements provides a potential novel, highly sensitive, and noninvasive method for the identification of atherosclerotic vulnerable plaques, aimed to develop a potential tool for in vivo tracking. Keywords: gold nanoparticles, macrophages, noninvasive detection, flow cytometry, vulnerable plaques
format	article
author	Ankri R Melzer S Tarnok A Fixler D
author_facet	Ankri R Melzer S Tarnok A Fixler D
author_sort	Ankri R
title	Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
title_short	Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
title_full	Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
title_fullStr	Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
title_full_unstemmed	Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
title_sort	detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/65bce4d04cbb492d93ebb032c17804dc
work_keys_str_mv	AT ankrir detectionofgoldnanorodsuptakebymacrophagesusingscatteringanalysescombinedwithdiffusionreflectionmeasurementsasapotentialtoolforinvivoatherosclerosistracking AT melzers detectionofgoldnanorodsuptakebymacrophagesusingscatteringanalysescombinedwithdiffusionreflectionmeasurementsasapotentialtoolforinvivoatherosclerosistracking AT tarnoka detectionofgoldnanorodsuptakebymacrophagesusingscatteringanalysescombinedwithdiffusionreflectionmeasurementsasapotentialtoolforinvivoatherosclerosistracking AT fixlerd detectionofgoldnanorodsuptakebymacrophagesusingscatteringanalysescombinedwithdiffusionreflectionmeasurementsasapotentialtoolforinvivoatherosclerosistracking
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Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking

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