In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance

Staphylococcus aureus can produce a multilayered biofilm embedded in extracellular polymeric matrix. This biofilm is difficult to remove, insensitive to antibiotics, easy to develop drug-resistant strains and causes enormous problems to environments and health. Phage lysin which commonly consists of...

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Autores principales:	Wei Hong, Raphael Nyaruaba, Xiaohong Li, Huan Liu, Hang Yang, Hongping Wei
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	real-time S. aureus biofilms lysins CBD CD Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/65c156b1c1444a4686b9d5fb87e8b131
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spelling	oai:doaj.org-article:65c156b1c1444a4686b9d5fb87e8b1312021-12-01T18:59:45ZIn-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance1664-302X10.3389/fmicb.2021.783472https://doaj.org/article/65c156b1c1444a4686b9d5fb87e8b1312021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.783472/fullhttps://doaj.org/toc/1664-302XStaphylococcus aureus can produce a multilayered biofilm embedded in extracellular polymeric matrix. This biofilm is difficult to remove, insensitive to antibiotics, easy to develop drug-resistant strains and causes enormous problems to environments and health. Phage lysin which commonly consists of a catalytic domain (CD) and a cell-wall binding domain (CBD) is a powerful weapon against bacterial biofilm. However, the real-time interaction between lysin and S. aureus biofilm is still not fully understood. In this study, we monitored the interactions of three lysins (ClyF, ClyC, PlySs2) against culture-on-chip S. aureus biofilm, in real-time, based on surface plasmon resonance (SPR). A typical SPR response curve showed that the lysins bound to the biofilm rapidly and the biofilm destruction started at a longer time. By using 1:1 binding model analysis, affinity constants (KD) for ClyF, ClyC, and PlySs2 were found to be 3.18 ± 0.127 μM, 1.12 ± 0.026 μM, and 15.5 ± 0.514 μM, respectively. The fact that ClyF and PlySs2 shared the same CBD but showed different affinity to S. aureus biofilm suggested that, not only CBD, but also CD affects the binding activity of the entire lysin. The SPR platform can be applied to improve our understanding on the complex interactions between lysins and bacterial biofilm including association (adsorption) and disassociation (destruction).Wei HongWei HongRaphael NyaruabaRaphael NyaruabaXiaohong LiXiaohong LiHuan LiuHuan LiuHang YangHang YangHongping WeiHongping WeiFrontiers Media S.A.articlereal-timeS. aureusbiofilmslysinsCBDCDMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time S. aureus biofilms lysins CBD CD Microbiology QR1-502
spellingShingle	real-time S. aureus biofilms lysins CBD CD Microbiology QR1-502 Wei Hong Wei Hong Raphael Nyaruaba Raphael Nyaruaba Xiaohong Li Xiaohong Li Huan Liu Huan Liu Hang Yang Hang Yang Hongping Wei Hongping Wei In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
description	Staphylococcus aureus can produce a multilayered biofilm embedded in extracellular polymeric matrix. This biofilm is difficult to remove, insensitive to antibiotics, easy to develop drug-resistant strains and causes enormous problems to environments and health. Phage lysin which commonly consists of a catalytic domain (CD) and a cell-wall binding domain (CBD) is a powerful weapon against bacterial biofilm. However, the real-time interaction between lysin and S. aureus biofilm is still not fully understood. In this study, we monitored the interactions of three lysins (ClyF, ClyC, PlySs2) against culture-on-chip S. aureus biofilm, in real-time, based on surface plasmon resonance (SPR). A typical SPR response curve showed that the lysins bound to the biofilm rapidly and the biofilm destruction started at a longer time. By using 1:1 binding model analysis, affinity constants (KD) for ClyF, ClyC, and PlySs2 were found to be 3.18 ± 0.127 μM, 1.12 ± 0.026 μM, and 15.5 ± 0.514 μM, respectively. The fact that ClyF and PlySs2 shared the same CBD but showed different affinity to S. aureus biofilm suggested that, not only CBD, but also CD affects the binding activity of the entire lysin. The SPR platform can be applied to improve our understanding on the complex interactions between lysins and bacterial biofilm including association (adsorption) and disassociation (destruction).
format	article
author	Wei Hong Wei Hong Raphael Nyaruaba Raphael Nyaruaba Xiaohong Li Xiaohong Li Huan Liu Huan Liu Hang Yang Hang Yang Hongping Wei Hongping Wei
author_facet	Wei Hong Wei Hong Raphael Nyaruaba Raphael Nyaruaba Xiaohong Li Xiaohong Li Huan Liu Huan Liu Hang Yang Hang Yang Hongping Wei Hongping Wei
author_sort	Wei Hong
title	In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
title_short	In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
title_full	In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
title_fullStr	In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
title_full_unstemmed	In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance
title_sort	in-situ and real-time monitoring of the interaction between lysins and staphylococcus aureus biofilm by surface plasmon resonance
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/65c156b1c1444a4686b9d5fb87e8b131
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In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance

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