Gene editing enables rapid engineering of complex antibiotic assembly lines

Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.

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Auteurs principaux:	Wei Li Thong, Yingxin Zhang, Ying Zhuo, Katherine J. Robins, Joanna K. Fyans, Abigail J. Herbert, Brian J. C. Law, Jason Micklefield
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/65ce6f9e89b844d7adc627e3f70e0f01
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id	oai:doaj.org-article:65ce6f9e89b844d7adc627e3f70e0f01
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spelling	oai:doaj.org-article:65ce6f9e89b844d7adc627e3f70e0f012021-11-28T12:34:55ZGene editing enables rapid engineering of complex antibiotic assembly lines10.1038/s41467-021-27139-12041-1723https://doaj.org/article/65ce6f9e89b844d7adc627e3f70e0f012021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27139-1https://doaj.org/toc/2041-1723Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.Wei Li ThongYingxin ZhangYing ZhuoKatherine J. RobinsJoanna K. FyansAbigail J. HerbertBrian J. C. LawJason MicklefieldNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wei Li Thong Yingxin Zhang Ying Zhuo Katherine J. Robins Joanna K. Fyans Abigail J. Herbert Brian J. C. Law Jason Micklefield Gene editing enables rapid engineering of complex antibiotic assembly lines
description	Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.
format	article
author	Wei Li Thong Yingxin Zhang Ying Zhuo Katherine J. Robins Joanna K. Fyans Abigail J. Herbert Brian J. C. Law Jason Micklefield
author_facet	Wei Li Thong Yingxin Zhang Ying Zhuo Katherine J. Robins Joanna K. Fyans Abigail J. Herbert Brian J. C. Law Jason Micklefield
author_sort	Wei Li Thong
title	Gene editing enables rapid engineering of complex antibiotic assembly lines
title_short	Gene editing enables rapid engineering of complex antibiotic assembly lines
title_full	Gene editing enables rapid engineering of complex antibiotic assembly lines
title_fullStr	Gene editing enables rapid engineering of complex antibiotic assembly lines
title_full_unstemmed	Gene editing enables rapid engineering of complex antibiotic assembly lines
title_sort	gene editing enables rapid engineering of complex antibiotic assembly lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65ce6f9e89b844d7adc627e3f70e0f01
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Gene editing enables rapid engineering of complex antibiotic assembly lines

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