Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia

Hina Qamar,1 Sumbul Rehman,2 Dushyant Kumar Chauhan,1 Ashok Kumar Tiwari,3 Vikramaditya Upmanyu3 1Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India; 2Department of Ilmul Advia (Unani Pharmacology), A.K. Tibbiya College, Aligarh Muslim University, Aligarh, Uttar P...

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Autores principales:	Qamar H, Rehman S, Chauhan DK, Tiwari AK, Upmanyu V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	copper oxide nanorods (cuo nrs) biosynthesis antibacterial antifungal antiviral trichophyton rubrum momordica charantia multidrug-resistance r2b newcastle disease virus x-ray diffraction ftir sem tem. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65d3238d17694416a3e2539abfec36ce
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id	oai:doaj.org-article:65d3238d17694416a3e2539abfec36ce
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	copper oxide nanorods (cuo nrs) biosynthesis antibacterial antifungal antiviral trichophyton rubrum momordica charantia multidrug-resistance r2b newcastle disease virus x-ray diffraction ftir sem tem. Medicine (General) R5-920
spellingShingle	copper oxide nanorods (cuo nrs) biosynthesis antibacterial antifungal antiviral trichophyton rubrum momordica charantia multidrug-resistance r2b newcastle disease virus x-ray diffraction ftir sem tem. Medicine (General) R5-920 Qamar H Rehman S Chauhan DK Tiwari AK Upmanyu V Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
description	Hina Qamar,1 Sumbul Rehman,2 Dushyant Kumar Chauhan,1 Ashok Kumar Tiwari,3 Vikramaditya Upmanyu3 1Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India; 2Department of Ilmul Advia (Unani Pharmacology), A.K. Tibbiya College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India; 3Biological Standardization Division, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, IndiaCorrespondence: Dushyant Kumar ChauhanDepartment of Zoology, Chaudhary Charan Singh University, Meerut 250001, Uttar Pradesh, IndiaTel +91 941 270 8983Email drdushyant.zoology@gmail.comBackground: In the emerging field of nanotechnology, copper oxide (CuO) nanomaterials are considered to be one of the most important transition metal oxides owing to its fascinating properties. Its synthesis from green chemistry principles is gaining importance as next-generation antibiotics due to its simplicity, eco-friendliness, and cost-effectiveness. In the present study, CuO nanorods (CuO NRs) were synthesized from the aqueous fruit extract of Momordica charantia and characterized using different analytical techniques. Further, the biomedical therapeutic potential was evaluated against multi-drug resistant microbial strains.Materials and Methods: To synthesize CuO NRs, 0.1M of CuSO4.5H2O solution was added to aqueous extract of Momordica charantia in a 1:3 (v/v) ratio (pH=11) and heated at 50°C followed by washing and drying. The synthesized CuO NRs were subjected to characterization using different analytical techniques such as UV visible spectroscopy, zeta sizer equipped with zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). Further, the application as a biomedical therapeutic potential was evaluated in vitro using well diffusion method against eleven multidrug-resistant clinical bacterial strains, a fungus- Trichophyton rubrum and in ovo against the R2B virus using haemagglutination (HA) test.Results: Characterization was preliminarily done by the spectral study that confirms the absorbance band at 245nm. FTIR analysis at 628 cm− 1 peak identified copper oxide vibration. SEM analysis revealed agglomerated particle clusters. However, with TEM clear nanorods of average diameter of 61.48 ± 2 nm were observed. EDAX confirmed CuO formation while XRD showed a typical monoclinic structure with 6 nm crystallite size. Biological screening of CuO NRs showed significant results against both in vitro and in ovo methods. Significant inhibitory activity (p< 0.0001) was noted against most of the resistant human pathogenic strains including both Gram-positive and Gram-negative bacteria. The highest efficacy was observed against Bacillus cereus with a 31.66 mm zone of inhibition. Besides, the therapeutic potential of CuO NRs against Corynebacterium xerosis, Streptococcus viridians and R2B strain of Newcastle disease is reported for the first time.Conclusion: Based on the present results, it could be expected that green synthesized CuO NRs would find potential applications in the field of nanomedicine.Keywords: copper oxide nanorods, CuO NRs, biosynthesis, antibacterial, antifungal, antiviral, Trichophyton rubrum, multidrug-resistance, TEM, R2B Newcastle disease virus
format	article
author	Qamar H Rehman S Chauhan DK Tiwari AK Upmanyu V
author_facet	Qamar H Rehman S Chauhan DK Tiwari AK Upmanyu V
author_sort	Qamar H
title	Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
title_short	Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
title_full	Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
title_fullStr	Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
title_full_unstemmed	Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia
title_sort	green synthesis, characterization and antimicrobial activity of copper oxide nanomaterial derived from momordica charantia
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/65d3238d17694416a3e2539abfec36ce
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spelling	oai:doaj.org-article:65d3238d17694416a3e2539abfec36ce2021-12-02T09:26:51ZGreen Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia1178-2013https://doaj.org/article/65d3238d17694416a3e2539abfec36ce2020-04-01T00:00:00Zhttps://www.dovepress.com/green-synthesis-characterization-and-antimicrobial-activity-of-copper--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hina Qamar,1 Sumbul Rehman,2 Dushyant Kumar Chauhan,1 Ashok Kumar Tiwari,3 Vikramaditya Upmanyu3 1Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India; 2Department of Ilmul Advia (Unani Pharmacology), A.K. Tibbiya College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India; 3Biological Standardization Division, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, IndiaCorrespondence: Dushyant Kumar ChauhanDepartment of Zoology, Chaudhary Charan Singh University, Meerut 250001, Uttar Pradesh, IndiaTel +91 941 270 8983Email drdushyant.zoology@gmail.comBackground: In the emerging field of nanotechnology, copper oxide (CuO) nanomaterials are considered to be one of the most important transition metal oxides owing to its fascinating properties. Its synthesis from green chemistry principles is gaining importance as next-generation antibiotics due to its simplicity, eco-friendliness, and cost-effectiveness. In the present study, CuO nanorods (CuO NRs) were synthesized from the aqueous fruit extract of Momordica charantia and characterized using different analytical techniques. Further, the biomedical therapeutic potential was evaluated against multi-drug resistant microbial strains.Materials and Methods: To synthesize CuO NRs, 0.1M of CuSO4.5H2O solution was added to aqueous extract of Momordica charantia in a 1:3 (v/v) ratio (pH=11) and heated at 50°C followed by washing and drying. The synthesized CuO NRs were subjected to characterization using different analytical techniques such as UV visible spectroscopy, zeta sizer equipped with zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). Further, the application as a biomedical therapeutic potential was evaluated in vitro using well diffusion method against eleven multidrug-resistant clinical bacterial strains, a fungus- Trichophyton rubrum and in ovo against the R2B virus using haemagglutination (HA) test.Results: Characterization was preliminarily done by the spectral study that confirms the absorbance band at 245nm. FTIR analysis at 628 cm− 1 peak identified copper oxide vibration. SEM analysis revealed agglomerated particle clusters. However, with TEM clear nanorods of average diameter of 61.48 ± 2 nm were observed. EDAX confirmed CuO formation while XRD showed a typical monoclinic structure with 6 nm crystallite size. Biological screening of CuO NRs showed significant results against both in vitro and in ovo methods. Significant inhibitory activity (p< 0.0001) was noted against most of the resistant human pathogenic strains including both Gram-positive and Gram-negative bacteria. The highest efficacy was observed against Bacillus cereus with a 31.66 mm zone of inhibition. Besides, the therapeutic potential of CuO NRs against Corynebacterium xerosis, Streptococcus viridians and R2B strain of Newcastle disease is reported for the first time.Conclusion: Based on the present results, it could be expected that green synthesized CuO NRs would find potential applications in the field of nanomedicine.Keywords: copper oxide nanorods, CuO NRs, biosynthesis, antibacterial, antifungal, antiviral, Trichophyton rubrum, multidrug-resistance, TEM, R2B Newcastle disease virusQamar HRehman SChauhan DKTiwari AKUpmanyu VDove Medical Pressarticlecopper oxide nanorods (cuo nrs)biosynthesisantibacterialantifungalantiviraltrichophyton rubrummomordica charantiamultidrug-resistancer2b newcastle disease virusx-ray diffractionftirsemtem.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2541-2553 (2020)

Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from Momordica charantia

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