Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity

In this laboratory experiment, we propose an opportunity for students to broaden their understanding of the ecology of antibiotic-resistant and sensitive waterborne bacteria. Antibiotics can be found in rivers or soil as a consequence of agricultural practices or as a result of human use. Concentrat...

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Autores principales:	Massimiliano Marvasi, Manika Choudhury, Nimisha Binesh Vala, Max Teplitski
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/65d5aabef14543aa9198491dc14d350e
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spelling	oai:doaj.org-article:65d5aabef14543aa9198491dc14d350e2021-11-15T15:04:11ZFitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity10.1128/jmbe.v18i1.12571935-78851935-7877https://doaj.org/article/65d5aabef14543aa9198491dc14d350e2017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i1.1257https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885In this laboratory experiment, we propose an opportunity for students to broaden their understanding of the ecology of antibiotic-resistant and sensitive waterborne bacteria. Antibiotics can be found in rivers or soil as a consequence of agricultural practices or as a result of human use. Concentrations of antibiotics in the environment may range from a few ng to μg L−1. Such concentrations can affect the selection and fitness of resistant bacteria. In this laboratory activity, students learn how to set up a fitness experiment by using an isogenic pair of antibiotic-resistant and sensitive bacteria in the presence or absence of selective pressure. Microcosms were generated by using filtered river water containing populations of resistant and sensitive bacteria. Competition of both populations was measured in the presence or absence of antibiotics. Students appreciated the use of microcosms for in vitro experiments and the extent to which the fitness of resistant and sensitive bacteria changed in the presence and/or absence of a selective pressure in river water. Student learning was measured by using different types of assessments: multiple-choice, true/false, fill in the blanks, laboratory skills observations, and laboratory reports. After the laboratory activity, the percentage of correct answers significantly rose from ~20% to ~85%. Laboratory skills were also evaluated during the exercises, showing no major issues during the experiment. Students showed proficiency in analyzing the complexity of fitness data by reaching a mean of 5.57 (standard error 0.57) over a maximum score of 7 points.Massimiliano MarvasiManika ChoudhuryNimisha Binesh ValaMax TeplitskiAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Massimiliano Marvasi Manika Choudhury Nimisha Binesh Vala Max Teplitski Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
description	In this laboratory experiment, we propose an opportunity for students to broaden their understanding of the ecology of antibiotic-resistant and sensitive waterborne bacteria. Antibiotics can be found in rivers or soil as a consequence of agricultural practices or as a result of human use. Concentrations of antibiotics in the environment may range from a few ng to μg L−1. Such concentrations can affect the selection and fitness of resistant bacteria. In this laboratory activity, students learn how to set up a fitness experiment by using an isogenic pair of antibiotic-resistant and sensitive bacteria in the presence or absence of selective pressure. Microcosms were generated by using filtered river water containing populations of resistant and sensitive bacteria. Competition of both populations was measured in the presence or absence of antibiotics. Students appreciated the use of microcosms for in vitro experiments and the extent to which the fitness of resistant and sensitive bacteria changed in the presence and/or absence of a selective pressure in river water. Student learning was measured by using different types of assessments: multiple-choice, true/false, fill in the blanks, laboratory skills observations, and laboratory reports. After the laboratory activity, the percentage of correct answers significantly rose from ~20% to ~85%. Laboratory skills were also evaluated during the exercises, showing no major issues during the experiment. Students showed proficiency in analyzing the complexity of fitness data by reaching a mean of 5.57 (standard error 0.57) over a maximum score of 7 points.
format	article
author	Massimiliano Marvasi Manika Choudhury Nimisha Binesh Vala Max Teplitski
author_facet	Massimiliano Marvasi Manika Choudhury Nimisha Binesh Vala Max Teplitski
author_sort	Massimiliano Marvasi
title	Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
title_short	Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
title_full	Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
title_fullStr	Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
title_full_unstemmed	Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity
title_sort	fitness of antibiotic-resistant bacteria in the environment: a laboratory activity
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/65d5aabef14543aa9198491dc14d350e
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Fitness of Antibiotic-Resistant Bacteria in the Environment: A Laboratory Activity

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