Barcode-free next-generation sequencing error validation for ultra-rare variant detection

Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.

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Autores principales:	Huiran Yeom, Yonghee Lee, Taehoon Ryu, Jinsung Noh, Amos Chungwon Lee, Han-Byoel Lee, Eunji Kang, Seo Woo Song, Sunghoon Kwon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/65d6e301df474af1aa557b932f32e833
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spelling	oai:doaj.org-article:65d6e301df474af1aa557b932f32e8332021-12-02T17:32:10ZBarcode-free next-generation sequencing error validation for ultra-rare variant detection10.1038/s41467-019-08941-42041-1723https://doaj.org/article/65d6e301df474af1aa557b932f32e8332019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08941-4https://doaj.org/toc/2041-1723Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.Huiran YeomYonghee LeeTaehoon RyuJinsung NohAmos Chungwon LeeHan-Byoel LeeEunji KangSeo Woo SongSunghoon KwonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Huiran Yeom Yonghee Lee Taehoon Ryu Jinsung Noh Amos Chungwon Lee Han-Byoel Lee Eunji Kang Seo Woo Song Sunghoon Kwon Barcode-free next-generation sequencing error validation for ultra-rare variant detection
description	Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.
format	article
author	Huiran Yeom Yonghee Lee Taehoon Ryu Jinsung Noh Amos Chungwon Lee Han-Byoel Lee Eunji Kang Seo Woo Song Sunghoon Kwon
author_facet	Huiran Yeom Yonghee Lee Taehoon Ryu Jinsung Noh Amos Chungwon Lee Han-Byoel Lee Eunji Kang Seo Woo Song Sunghoon Kwon
author_sort	Huiran Yeom
title	Barcode-free next-generation sequencing error validation for ultra-rare variant detection
title_short	Barcode-free next-generation sequencing error validation for ultra-rare variant detection
title_full	Barcode-free next-generation sequencing error validation for ultra-rare variant detection
title_fullStr	Barcode-free next-generation sequencing error validation for ultra-rare variant detection
title_full_unstemmed	Barcode-free next-generation sequencing error validation for ultra-rare variant detection
title_sort	barcode-free next-generation sequencing error validation for ultra-rare variant detection
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/65d6e301df474af1aa557b932f32e833
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Barcode-free next-generation sequencing error validation for ultra-rare variant detection

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