Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches

Abstract Acinetobacter baumannii (A. baumannii), an opportunistic, gram-negative pathogen, has evoked the interest of the medical community throughout the world because of its ability to cause nosocomial infections, majorly infecting those in intensive care units. It has also drawn the attention of...

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Autores principales:	Fatima Shahid, Tahreem Zaheer, Shifa Tariq Ashraf, Muhammad Shehroz, Farha Anwer, Anam Naz, Amjad Ali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65d736c411bb4c2b860a32d9fa8c1b62
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spelling	oai:doaj.org-article:65d736c411bb4c2b860a32d9fa8c1b622021-12-02T18:02:54ZChimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches10.1038/s41598-021-92501-82045-2322https://doaj.org/article/65d736c411bb4c2b860a32d9fa8c1b622021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92501-8https://doaj.org/toc/2045-2322Abstract Acinetobacter baumannii (A. baumannii), an opportunistic, gram-negative pathogen, has evoked the interest of the medical community throughout the world because of its ability to cause nosocomial infections, majorly infecting those in intensive care units. It has also drawn the attention of researchers due to its evolving immune evasion strategies and increased drug resistance. The emergence of multi-drug-resistant-strains has urged the need to explore novel therapeutic options as an alternative to antibiotics. Due to the upsurge in antibiotic resistance mechanisms exhibited by A. baumannii, the current therapeutic strategies are rendered less effective. The aim of this study is to explore novel therapeutic alternatives against A. baumannii to control the ailed infection. In this study, a computational framework is employed involving, pan genomics, subtractive proteomics and reverse vaccinology strategies to identify core promiscuous vaccine candidates. Two chimeric vaccine constructs having B-cell derived T-cell epitopes from prioritized vaccine candidates; APN, AdeK and AdeI have been designed and checked for their possible interactions with host BCR, TLRs and HLA Class I and II Superfamily alleles. These vaccine candidates can be experimentally validated and thus contribute to vaccine development against A. baumannii infections.Fatima ShahidTahreem ZaheerShifa Tariq AshrafMuhammad ShehrozFarha AnwerAnam NazAmjad AliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fatima Shahid Tahreem Zaheer Shifa Tariq Ashraf Muhammad Shehroz Farha Anwer Anam Naz Amjad Ali Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
description	Abstract Acinetobacter baumannii (A. baumannii), an opportunistic, gram-negative pathogen, has evoked the interest of the medical community throughout the world because of its ability to cause nosocomial infections, majorly infecting those in intensive care units. It has also drawn the attention of researchers due to its evolving immune evasion strategies and increased drug resistance. The emergence of multi-drug-resistant-strains has urged the need to explore novel therapeutic options as an alternative to antibiotics. Due to the upsurge in antibiotic resistance mechanisms exhibited by A. baumannii, the current therapeutic strategies are rendered less effective. The aim of this study is to explore novel therapeutic alternatives against A. baumannii to control the ailed infection. In this study, a computational framework is employed involving, pan genomics, subtractive proteomics and reverse vaccinology strategies to identify core promiscuous vaccine candidates. Two chimeric vaccine constructs having B-cell derived T-cell epitopes from prioritized vaccine candidates; APN, AdeK and AdeI have been designed and checked for their possible interactions with host BCR, TLRs and HLA Class I and II Superfamily alleles. These vaccine candidates can be experimentally validated and thus contribute to vaccine development against A. baumannii infections.
format	article
author	Fatima Shahid Tahreem Zaheer Shifa Tariq Ashraf Muhammad Shehroz Farha Anwer Anam Naz Amjad Ali
author_facet	Fatima Shahid Tahreem Zaheer Shifa Tariq Ashraf Muhammad Shehroz Farha Anwer Anam Naz Amjad Ali
author_sort	Fatima Shahid
title	Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
title_short	Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
title_full	Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
title_fullStr	Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
title_full_unstemmed	Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches
title_sort	chimeric vaccine designs against acinetobacter baumannii using pan genome and reverse vaccinology approaches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65d736c411bb4c2b860a32d9fa8c1b62
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Chimeric vaccine designs against Acinetobacter baumannii using pan genome and reverse vaccinology approaches

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