CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression

T cells and endothelial cells engage in bidirectional communication that regulates angiogenesis and T cell transmigration. Extracellular vesicles (EVs) mediate intercellular communication by the transfer of bioactive molecules including RNAs. EVs produced by a given cell type are heterogeneous in th...

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Autores principales:	Sukhbir Kaur, Abdel G. Elkahloun, Jennifer D. Petersen, Anush Arakelyan, Ferenc Livak, Satya P. Singh, Leonid Margolis, Joshua Zimmerberg, David D. Roberts
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	extracellular vesicles non-coding RNAs cell-cell communication Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/65dcaa0b024640f7ab18112353ea5d40
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spelling	oai:doaj.org-article:65dcaa0b024640f7ab18112353ea5d402021-11-25T16:51:00ZCD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression10.3390/biomedicines91117052227-9059https://doaj.org/article/65dcaa0b024640f7ab18112353ea5d402021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1705https://doaj.org/toc/2227-9059T cells and endothelial cells engage in bidirectional communication that regulates angiogenesis and T cell transmigration. Extracellular vesicles (EVs) mediate intercellular communication by the transfer of bioactive molecules including RNAs. EVs produced by a given cell type are heterogeneous in their RNA content, but it is unclear how specific EV surface markers relate to their functional effects on target cells. Our previous work established that Jurkat T cell EVs bearing CD63, MHC-I, or CD47 surface markers contain distinct noncoding RNA populations. The present study reveals that CD63<sup>+</sup> and MHC-I<sup>+</sup> EVs from CD47-deficient Jurkat T cells are enriched in small non-coding RNAs relative to EVs from wild-type Jurkat T cells. CD47-deficient Jurkat T cells secrete more CD63<sup>+</sup> and MHC-I<sup>+</sup> EVs, but MHC-I<sup>+</sup> EVs are selectively taken up more by human umbilical vein endothelial cells. Transcriptomics analysis of endothelial cells treated with CD63<sup>+</sup> or MHC-I<sup>+</sup> EVs showed surface marker- and CD47-dependent changes in gene expression in the target cells. Gene set enrichment analysis identified CD47-dependent, and surface marker-dependent effects of T cell EVs on VEGF and inflammatory signaling, cell cycle, and lipid and cholesterol metabolism. Thus, subsets of T cell EVs differentially regulate endothelial cell metabolism and inflammatory and angiogenic responses.Sukhbir KaurAbdel G. ElkahlounJennifer D. PetersenAnush ArakelyanFerenc LivakSatya P. SinghLeonid MargolisJoshua ZimmerbergDavid D. RobertsMDPI AGarticleextracellular vesiclesnon-coding RNAscell-cell communicationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1705, p 1705 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles non-coding RNAs cell-cell communication Biology (General) QH301-705.5
spellingShingle	extracellular vesicles non-coding RNAs cell-cell communication Biology (General) QH301-705.5 Sukhbir Kaur Abdel G. Elkahloun Jennifer D. Petersen Anush Arakelyan Ferenc Livak Satya P. Singh Leonid Margolis Joshua Zimmerberg David D. Roberts CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
description	T cells and endothelial cells engage in bidirectional communication that regulates angiogenesis and T cell transmigration. Extracellular vesicles (EVs) mediate intercellular communication by the transfer of bioactive molecules including RNAs. EVs produced by a given cell type are heterogeneous in their RNA content, but it is unclear how specific EV surface markers relate to their functional effects on target cells. Our previous work established that Jurkat T cell EVs bearing CD63, MHC-I, or CD47 surface markers contain distinct noncoding RNA populations. The present study reveals that CD63<sup>+</sup> and MHC-I<sup>+</sup> EVs from CD47-deficient Jurkat T cells are enriched in small non-coding RNAs relative to EVs from wild-type Jurkat T cells. CD47-deficient Jurkat T cells secrete more CD63<sup>+</sup> and MHC-I<sup>+</sup> EVs, but MHC-I<sup>+</sup> EVs are selectively taken up more by human umbilical vein endothelial cells. Transcriptomics analysis of endothelial cells treated with CD63<sup>+</sup> or MHC-I<sup>+</sup> EVs showed surface marker- and CD47-dependent changes in gene expression in the target cells. Gene set enrichment analysis identified CD47-dependent, and surface marker-dependent effects of T cell EVs on VEGF and inflammatory signaling, cell cycle, and lipid and cholesterol metabolism. Thus, subsets of T cell EVs differentially regulate endothelial cell metabolism and inflammatory and angiogenic responses.
format	article
author	Sukhbir Kaur Abdel G. Elkahloun Jennifer D. Petersen Anush Arakelyan Ferenc Livak Satya P. Singh Leonid Margolis Joshua Zimmerberg David D. Roberts
author_facet	Sukhbir Kaur Abdel G. Elkahloun Jennifer D. Petersen Anush Arakelyan Ferenc Livak Satya P. Singh Leonid Margolis Joshua Zimmerberg David D. Roberts
author_sort	Sukhbir Kaur
title	CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
title_short	CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
title_full	CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
title_fullStr	CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
title_full_unstemmed	CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression
title_sort	cd63<sup>+</sup> and mhc class i<sup>+</sup> subsets of extracellular vesicles produced by wild-type and cd47-deficient jurkat t cells have divergent functional effects on endothelial cell gene expression
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/65dcaa0b024640f7ab18112353ea5d40
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CD63<sup>+</sup> and MHC Class I<sup>+</sup> Subsets of Extracellular Vesicles Produced by Wild-Type and CD47-Deficient Jurkat T Cells Have Divergent Functional Effects on Endothelial Cell Gene Expression

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