Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid

Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid

Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon University, Seongnam, South Korea; 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: Oxidative stress can damage proteins, DNA, and l...

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Autores principales: Nanda SS, An SS, Yi DK
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/65dfb3bb825a41c186eb7237c1c79acc
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spelling oai:doaj.org-article:65dfb3bb825a41c186eb7237c1c79acc2021-12-02T03:58:40ZOxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid1178-2013https://doaj.org/article/65dfb3bb825a41c186eb7237c1c79acc2015-01-01T00:00:00Zhttp://www.dovepress.com/oxidative-stress-and-antibacterial-properties-ofnbspanbspgraphene-oxid-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon University, Seongnam, South Korea; 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: Oxidative stress can damage proteins, DNA, and lipids, and is involved in the progression of many diseases. Damage to infected cells caused by oxidative stress is related to increased levels of reactive oxygen species, including hydrogen peroxide. During oxidative stress, hydrogen peroxide levels are often increased and catalase level decreased inside cells. This can lead to the death of skin and other cells. Hydrophobic low molecular weight compounds are useful in treating hemorrhagic conditions of the skin. To this end, cystamine has been successfully conjugated with graphene oxide (GO) as a drug carrier. The current study used the microdilution method to determine the minimum inhibitory concentrations of cystamine-conjugated GO against four types of pathogenic bacteria. Minimum inhibitory concentrations values were 1 µg/mL against Escherichia coli and Salmonella typhimurium, 6 µg/mL against Enterococcus faecalis, and 4 µg/mL against Bacillus subtilis. Toxicity of the conjugate against squamous cell carcinoma 7 cells was minimal at low concentrations, but increased in a dose-dependent manner. These results demonstrated that our protocol produced a cystamine-conjugated GO with low cytotoxicity, but strong reactive oxygen species effects and high antibacterial activity. This nanohybrid may be useful in the treatment of dermatological disorders. Moreover, this class of nanohybrid may have other biomedical applications due to their low cytotoxicity and high antibacterial activity. Keywords: graphene, oxide, cystamine conjugate, reactive oxygen species, antibacterial, oxidative stressNanda SSAn SSYi DKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 549-556 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Nanda SS
An SS
Yi DK
Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
description Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon University, Seongnam, South Korea; 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: Oxidative stress can damage proteins, DNA, and lipids, and is involved in the progression of many diseases. Damage to infected cells caused by oxidative stress is related to increased levels of reactive oxygen species, including hydrogen peroxide. During oxidative stress, hydrogen peroxide levels are often increased and catalase level decreased inside cells. This can lead to the death of skin and other cells. Hydrophobic low molecular weight compounds are useful in treating hemorrhagic conditions of the skin. To this end, cystamine has been successfully conjugated with graphene oxide (GO) as a drug carrier. The current study used the microdilution method to determine the minimum inhibitory concentrations of cystamine-conjugated GO against four types of pathogenic bacteria. Minimum inhibitory concentrations values were 1 µg/mL against Escherichia coli and Salmonella typhimurium, 6 µg/mL against Enterococcus faecalis, and 4 µg/mL against Bacillus subtilis. Toxicity of the conjugate against squamous cell carcinoma 7 cells was minimal at low concentrations, but increased in a dose-dependent manner. These results demonstrated that our protocol produced a cystamine-conjugated GO with low cytotoxicity, but strong reactive oxygen species effects and high antibacterial activity. This nanohybrid may be useful in the treatment of dermatological disorders. Moreover, this class of nanohybrid may have other biomedical applications due to their low cytotoxicity and high antibacterial activity. Keywords: graphene, oxide, cystamine conjugate, reactive oxygen species, antibacterial, oxidative stress
format article
author Nanda SS
An SS
Yi DK
author_facet Nanda SS
An SS
Yi DK
author_sort Nanda SS
title Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
title_short Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
title_full Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
title_fullStr Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
title_full_unstemmed Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
title_sort oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/65dfb3bb825a41c186eb7237c1c79acc
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