Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes

Abstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this...

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Autores principales:	Min‐Hua Yao, Wan‐Jing Cheng, Li‐Wei Liu, Hui Zheng, Wan‐Ying Gu, Fang Miao, Jing‐Fang Zhang, Li Wang, Yan‐Ping Su, Ya‐Ling Liu, Hong‐Shu Sui
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	chromatin configuration competence of maturation germinal vesicle guinea pig oocyte Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/65e19e9147724b3cb9c8fb9c3f3f01eb
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spelling	oai:doaj.org-article:65e19e9147724b3cb9c8fb9c3f3f01eb2021-11-19T17:14:25ZRelationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes2053-109510.1002/vms3.596https://doaj.org/article/65e19e9147724b3cb9c8fb9c3f3f01eb2021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.596https://doaj.org/toc/2053-1095Abstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this study on the in vitro maturation (IVM) of guinea pig oocytes, we examined the changes in their GV chromatin configurations during meiotic progression. Methods Based on the degree of chromatin compaction, the GV chromatin configurations of guinea pig oocytes could be divided into three categories depending on whether the nucleolus‐like body (NLB) was surrounded or partly surrounded by compacted chromatin, namely the uncondensed (NSN), the intermediate type (SN‐1) and the compacted type (SN‐2). Results The percentage of cells displaying the SN‐2 configuration increased with the growth of guinea pig oocytes, suggesting that this configuration presents the potential for maturation in oocytes. Oocytes derived from larger follicle exhibited increased meiotic potential. Serum starvation affected the GV chromatin configurations of guinea pig oocytes. Conclusions Collectively, these results suggest that the SN‐2 type might be a more mature form of configuration in guinea pig oocyte, whose proportion was associated with the follicle size and susceptible to the environment (e.g. serum concentration).Min‐Hua YaoWan‐Jing ChengLi‐Wei LiuHui ZhengWan‐Ying GuFang MiaoJing‐Fang ZhangLi WangYan‐Ping SuYa‐Ling LiuHong‐Shu SuiWileyarticlechromatin configurationcompetence of maturationgerminal vesicleguinea pig oocyteVeterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2410-2417 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromatin configuration competence of maturation germinal vesicle guinea pig oocyte Veterinary medicine SF600-1100
spellingShingle	chromatin configuration competence of maturation germinal vesicle guinea pig oocyte Veterinary medicine SF600-1100 Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
description	Abstract Background Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes. Objective In this study on the in vitro maturation (IVM) of guinea pig oocytes, we examined the changes in their GV chromatin configurations during meiotic progression. Methods Based on the degree of chromatin compaction, the GV chromatin configurations of guinea pig oocytes could be divided into three categories depending on whether the nucleolus‐like body (NLB) was surrounded or partly surrounded by compacted chromatin, namely the uncondensed (NSN), the intermediate type (SN‐1) and the compacted type (SN‐2). Results The percentage of cells displaying the SN‐2 configuration increased with the growth of guinea pig oocytes, suggesting that this configuration presents the potential for maturation in oocytes. Oocytes derived from larger follicle exhibited increased meiotic potential. Serum starvation affected the GV chromatin configurations of guinea pig oocytes. Conclusions Collectively, these results suggest that the SN‐2 type might be a more mature form of configuration in guinea pig oocyte, whose proportion was associated with the follicle size and susceptible to the environment (e.g. serum concentration).
format	article
author	Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui
author_facet	Min‐Hua Yao Wan‐Jing Cheng Li‐Wei Liu Hui Zheng Wan‐Ying Gu Fang Miao Jing‐Fang Zhang Li Wang Yan‐Ping Su Ya‐Ling Liu Hong‐Shu Sui
author_sort	Min‐Hua Yao
title	Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
title_short	Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
title_full	Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
title_fullStr	Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
title_full_unstemmed	Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
title_sort	relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/65e19e9147724b3cb9c8fb9c3f3f01eb
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Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes

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