Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response

Background. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of spec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	V. I. Dubrovina, K. M. Korytov, A. B. Petyatestnikova, N. O. Kiseleva, V. V. Voitkova, D. D. Bryukhova, V. B. Nikolaev, V. S. Polovinkina, L. K. Salchak, L. V. Shchuchinov, S. V. Balakhonov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	antigens lymphocytes live plague vaccine cytokines immunity Science Q
Acceso en línea:	https://doaj.org/article/65e25fc7ee8e4f53a996b34072e9c9fa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65e25fc7ee8e4f53a996b34072e9c9fa
record_format	dspace
spelling	oai:doaj.org-article:65e25fc7ee8e4f53a996b34072e9c9fa2021-11-23T06:14:45ZExperience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response2541-94202587-959610.29413/ABS.2021-6.2.4https://doaj.org/article/65e25fc7ee8e4f53a996b34072e9c9fa2021-06-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2727https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of specific antibodies to the capsular antigen (F1) ofthe plague microbe with other indicators of the state of cellular defense reactivity indicates the need to search for new informative and accessible markers for assessing anti-plague immunity.Objective: to evaluate possibility of using the complex preparation (F1 and cell membranes) evaluate the possibilities of using an artificial antigenic complex based on F1 and cell membranes (CM) of the plague microbe in antigen-specific tests in vitro in people vaccinated against plague.resu. The study involved 153 volunteers living in the territory enzootic for plague (the village of Khandagayty ofthe Ovyur kozhuun of the Tyva Republic and the village of Kosh-Agach of the Kosh-Agach district of the Altai Republic). The study included the determination of spontaneous and mitogen-induced production of cytokines (IFN-γ, IL-4, TNF-α) by blood cells, titers of specific IgG antibodies to the capsular antigen F1 of the plague microbe and concentrations ofthe main classes of immunoglobulins (IgM, IgG, IgA and IgE) in blood serum, as well as immunophenotyping of blood lymphocytes (CD3, CD4, CD8, CD16, CD19).Results. Comparative assessment of the level of cytokines (TNF-α, IFN-γ and IL-4) in spontaneous/induced F1+CM Y. pestis tests revealed a statistically significant increase in the production of cytokines TNF-α and IFN-γ in the antigeninduced tests compared with spontaneous (p < 0.01).Conclusion. Thus, the effectiveness of the use of artificial antigenic complex based on F1 and cell membranes ofthe plague microbe has been shown to assess the production of cytokines in antigen-specific cell tests in vitro, which justifies the need for further research.V. I. DubrovinaK. M. KorytovA. B. PetyatestnikovaN. O. KiselevaV. V. VoitkovaD. D. BryukhovaV. B. NikolaevV. S. PolovinkinaL. K. SalchakL. V. ShchuchinovS. V. BalakhonovScientific Сentre for Family Health and Human Reproduction Problemsarticleantigenslymphocyteslive plague vaccinecytokinesimmunityScienceQRUActa Biomedica Scientifica, Vol 6, Iss 2, Pp 41-46 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	antigens lymphocytes live plague vaccine cytokines immunity Science Q
spellingShingle	antigens lymphocytes live plague vaccine cytokines immunity Science Q V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
description	Background. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of specific antibodies to the capsular antigen (F1) ofthe plague microbe with other indicators of the state of cellular defense reactivity indicates the need to search for new informative and accessible markers for assessing anti-plague immunity.Objective: to evaluate possibility of using the complex preparation (F1 and cell membranes) evaluate the possibilities of using an artificial antigenic complex based on F1 and cell membranes (CM) of the plague microbe in antigen-specific tests in vitro in people vaccinated against plague.resu. The study involved 153 volunteers living in the territory enzootic for plague (the village of Khandagayty ofthe Ovyur kozhuun of the Tyva Republic and the village of Kosh-Agach of the Kosh-Agach district of the Altai Republic). The study included the determination of spontaneous and mitogen-induced production of cytokines (IFN-γ, IL-4, TNF-α) by blood cells, titers of specific IgG antibodies to the capsular antigen F1 of the plague microbe and concentrations ofthe main classes of immunoglobulins (IgM, IgG, IgA and IgE) in blood serum, as well as immunophenotyping of blood lymphocytes (CD3, CD4, CD8, CD16, CD19).Results. Comparative assessment of the level of cytokines (TNF-α, IFN-γ and IL-4) in spontaneous/induced F1+CM Y. pestis tests revealed a statistically significant increase in the production of cytokines TNF-α and IFN-γ in the antigeninduced tests compared with spontaneous (p < 0.01).Conclusion. Thus, the effectiveness of the use of artificial antigenic complex based on F1 and cell membranes ofthe plague microbe has been shown to assess the production of cytokines in antigen-specific cell tests in vitro, which justifies the need for further research.
format	article
author	V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov
author_facet	V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov
author_sort	V. I. Dubrovina
title	Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
title_short	Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
title_full	Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
title_fullStr	Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
title_full_unstemmed	Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
title_sort	experience of using a complex antigenic preparation of the plague microbe to assess the severity of a specific anti-plague response
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/65e25fc7ee8e4f53a996b34072e9c9fa
work_keys_str_mv	AT vidubrovina experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT kmkorytov experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT abpetyatestnikova experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT nokiseleva experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT vvvoitkova experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT ddbryukhova experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT vbnikolaev experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT vspolovinkina experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT lksalchak experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT lvshchuchinov experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse AT svbalakhonov experienceofusingacomplexantigenicpreparationoftheplaguemicrobetoassesstheseverityofaspecificantiplagueresponse
_version_	1718416884927299584

Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response

Ejemplares similares