Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products

A key step in the process of isolating microbial natural products is the preparation of an extract from a culture. This step determines which molecules will be available for detection in the subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we wanted to doc...

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Autores principales:	Yannik K. Schneider, Solveig M. Jørgensen, Jeanette Hammer Andersen, Espen H. Hansen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	natural products bacteria downstream processing antibiotics isolation extraction Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/65e41223b24c42a1a01c41d9234c0356
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spelling	oai:doaj.org-article:65e41223b24c42a1a01c41d9234c03562021-11-11T15:17:01ZQualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products10.3390/app1121102412076-3417https://doaj.org/article/65e41223b24c42a1a01c41d9234c03562021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10241https://doaj.org/toc/2076-3417A key step in the process of isolating microbial natural products is the preparation of an extract from a culture. This step determines which molecules will be available for detection in the subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we wanted to document potential differences in performance between liquid–liquid extraction using ethyl acetate and liquid–solid extraction using a poly-benzyl-resin. For the comparison of the two extraction protocols, we spiked a culture of <i>Flavobacterium</i> sp. with a diverse selection of natural products of microbial and plant origin to investigate whether the methods were comparable with respect to selectivity. We also investigated the efficiency of the two extraction methods quantitatively, using water spiked with a selection of natural products, and studied the quantitative effect of different pH levels of the aqueous solutions on the extraction yields of the two methods. The same compounds were extracted by the two methods, but the solid-phase extract contained more media components compared with the liquid-phase extract. Quantitatively, the two extraction methods varied in their recovery rates. We conclude that practical aspects could be more important when selecting one of the extraction protocols, as their efficiencies in extracting specific compounds were quite similar.Yannik K. SchneiderSolveig M. JørgensenJeanette Hammer AndersenEspen H. HansenMDPI AGarticlenatural productsbacteriadownstream processingantibioticsisolationextractionTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10241, p 10241 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	natural products bacteria downstream processing antibiotics isolation extraction Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	natural products bacteria downstream processing antibiotics isolation extraction Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Yannik K. Schneider Solveig M. Jørgensen Jeanette Hammer Andersen Espen H. Hansen Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
description	A key step in the process of isolating microbial natural products is the preparation of an extract from a culture. This step determines which molecules will be available for detection in the subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we wanted to document potential differences in performance between liquid–liquid extraction using ethyl acetate and liquid–solid extraction using a poly-benzyl-resin. For the comparison of the two extraction protocols, we spiked a culture of <i>Flavobacterium</i> sp. with a diverse selection of natural products of microbial and plant origin to investigate whether the methods were comparable with respect to selectivity. We also investigated the efficiency of the two extraction methods quantitatively, using water spiked with a selection of natural products, and studied the quantitative effect of different pH levels of the aqueous solutions on the extraction yields of the two methods. The same compounds were extracted by the two methods, but the solid-phase extract contained more media components compared with the liquid-phase extract. Quantitatively, the two extraction methods varied in their recovery rates. We conclude that practical aspects could be more important when selecting one of the extraction protocols, as their efficiencies in extracting specific compounds were quite similar.
format	article
author	Yannik K. Schneider Solveig M. Jørgensen Jeanette Hammer Andersen Espen H. Hansen
author_facet	Yannik K. Schneider Solveig M. Jørgensen Jeanette Hammer Andersen Espen H. Hansen
author_sort	Yannik K. Schneider
title	Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
title_short	Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
title_full	Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
title_fullStr	Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
title_full_unstemmed	Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
title_sort	qualitative and quantitative comparison of liquid–liquid phase extraction using ethyl acetate and liquid–solid phase extraction using poly-benzyl-resin for natural products
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/65e41223b24c42a1a01c41d9234c0356
work_keys_str_mv	AT yannikkschneider qualitativeandquantitativecomparisonofliquidliquidphaseextractionusingethylacetateandliquidsolidphaseextractionusingpolybenzylresinfornaturalproducts AT solveigmjørgensen qualitativeandquantitativecomparisonofliquidliquidphaseextractionusingethylacetateandliquidsolidphaseextractionusingpolybenzylresinfornaturalproducts AT jeanettehammerandersen qualitativeandquantitativecomparisonofliquidliquidphaseextractionusingethylacetateandliquidsolidphaseextractionusingpolybenzylresinfornaturalproducts AT espenhhansen qualitativeandquantitativecomparisonofliquidliquidphaseextractionusingethylacetateandliquidsolidphaseextractionusingpolybenzylresinfornaturalproducts
_version_	1718435756315246592

Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products

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