Metabolic changes of Interleukin-12/15/18-stimulated human NK cells

Abstract Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their...

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Autores principales:	Iñigo Terrén, Ane Orrantia, Alba Mosteiro, Joana Vitallé, Olatz Zenarruzabeitia, Francisco Borrego
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65ecfe7c7b8b4d3684f1ec5ff7a050c6
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spelling	oai:doaj.org-article:65ecfe7c7b8b4d3684f1ec5ff7a050c62021-12-02T11:39:47ZMetabolic changes of Interleukin-12/15/18-stimulated human NK cells10.1038/s41598-021-85960-62045-2322https://doaj.org/article/65ecfe7c7b8b4d3684f1ec5ff7a050c62021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85960-6https://doaj.org/toc/2045-2322Abstract Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their persistence in the host and their increased effector functions. Several studies have shown that NK cells modulate their metabolism in response to cytokine-stimulation and other stimuli, suggesting that there is a link between metabolism and cellular functions. In this paper, we have analyzed metabolic changes associated to IL-12/15/18-stimulation and the relevance of glycolytic pathway for NK cell effector functions. We have found CIML NK cells are able to retain a metabolic profile shifted towards glycolysis seven days after cytokine withdrawal. Furthermore, we found that treatment with 2-DG differently affects distinct NK cell effector functions and is stimuli-dependent. These findings may have implications in the design of NK cell-based cancer immunotherapies.Iñigo TerrénAne OrrantiaAlba MosteiroJoana VitalléOlatz ZenarruzabeitiaFrancisco BorregoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iñigo Terrén Ane Orrantia Alba Mosteiro Joana Vitallé Olatz Zenarruzabeitia Francisco Borrego Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
description	Abstract Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their persistence in the host and their increased effector functions. Several studies have shown that NK cells modulate their metabolism in response to cytokine-stimulation and other stimuli, suggesting that there is a link between metabolism and cellular functions. In this paper, we have analyzed metabolic changes associated to IL-12/15/18-stimulation and the relevance of glycolytic pathway for NK cell effector functions. We have found CIML NK cells are able to retain a metabolic profile shifted towards glycolysis seven days after cytokine withdrawal. Furthermore, we found that treatment with 2-DG differently affects distinct NK cell effector functions and is stimuli-dependent. These findings may have implications in the design of NK cell-based cancer immunotherapies.
format	article
author	Iñigo Terrén Ane Orrantia Alba Mosteiro Joana Vitallé Olatz Zenarruzabeitia Francisco Borrego
author_facet	Iñigo Terrén Ane Orrantia Alba Mosteiro Joana Vitallé Olatz Zenarruzabeitia Francisco Borrego
author_sort	Iñigo Terrén
title	Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
title_short	Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
title_full	Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
title_fullStr	Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
title_full_unstemmed	Metabolic changes of Interleukin-12/15/18-stimulated human NK cells
title_sort	metabolic changes of interleukin-12/15/18-stimulated human nk cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65ecfe7c7b8b4d3684f1ec5ff7a050c6
work_keys_str_mv	AT inigoterren metabolicchangesofinterleukin121518stimulatedhumannkcells AT aneorrantia metabolicchangesofinterleukin121518stimulatedhumannkcells AT albamosteiro metabolicchangesofinterleukin121518stimulatedhumannkcells AT joanavitalle metabolicchangesofinterleukin121518stimulatedhumannkcells AT olatzzenarruzabeitia metabolicchangesofinterleukin121518stimulatedhumannkcells AT franciscoborrego metabolicchangesofinterleukin121518stimulatedhumannkcells
_version_	1718395703858823168

Metabolic changes of Interleukin-12/15/18-stimulated human NK cells

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