Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense

Abstract Recent studies have characterized a dominant clone (Clone 1) of Mycobacterium abscessus subspecies massiliense (M. massiliense) associated with high prevalence in cystic fibrosis (CF) patients, pulmonary outbreaks in the United States (US) and United Kingdom (UK), and a Brazilian epidemic o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rebecca M. Davidson, Jeanne B. Benoit, Sara M. Kammlade, Nabeeh A. Hasan, L. Elaine Epperson, Terry Smith, Sruthi Vasireddy, Barbara A. Brown-Elliott, Jerry A. Nick, Kenneth N. Olivier, Adrian M. Zelazny, Charles L. Daley, Michael Strong, Richard J. Wallace
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65f02a743ac340e2baeb45d175365d25
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65f02a743ac340e2baeb45d175365d25
record_format	dspace
spelling	oai:doaj.org-article:65f02a743ac340e2baeb45d175365d252021-12-02T16:31:47ZGenomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense10.1038/s41598-021-94789-y2045-2322https://doaj.org/article/65f02a743ac340e2baeb45d175365d252021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94789-yhttps://doaj.org/toc/2045-2322Abstract Recent studies have characterized a dominant clone (Clone 1) of Mycobacterium abscessus subspecies massiliense (M. massiliense) associated with high prevalence in cystic fibrosis (CF) patients, pulmonary outbreaks in the United States (US) and United Kingdom (UK), and a Brazilian epidemic of skin infections. The prevalence of Clone 1 in non-CF patients in the US and the relationship of sporadic US isolates to outbreak clones are not known. We surveyed a reference US Mycobacteria Laboratory and a US biorepository of CF-associated Mycobacteria isolates for Clone 1. We then compared genomic variation and antimicrobial resistance (AMR) mutations between sporadic non-CF, CF, and outbreak Clone 1 isolates. Among reference lab samples, 57/147 (39%) of patients with M. massiliense had Clone 1, including pulmonary and extrapulmonary infections, compared to 11/64 (17%) in the CF isolate biorepository. Core and pan genome analyses revealed that outbreak isolates had similar numbers of single nucleotide polymorphisms (SNPs) and accessory genes as sporadic US Clone 1 isolates. However, pulmonary outbreak isolates were more likely to have AMR mutations compared to sporadic isolates. Clone 1 isolates are present among non-CF and CF patients across the US, but additional studies will be needed to resolve potential routes of transmission and spread.Rebecca M. DavidsonJeanne B. BenoitSara M. KammladeNabeeh A. HasanL. Elaine EppersonTerry SmithSruthi VasireddyBarbara A. Brown-ElliottJerry A. NickKenneth N. OlivierAdrian M. ZelaznyCharles L. DaleyMichael StrongRichard J. WallaceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebecca M. Davidson Jeanne B. Benoit Sara M. Kammlade Nabeeh A. Hasan L. Elaine Epperson Terry Smith Sruthi Vasireddy Barbara A. Brown-Elliott Jerry A. Nick Kenneth N. Olivier Adrian M. Zelazny Charles L. Daley Michael Strong Richard J. Wallace Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
description	Abstract Recent studies have characterized a dominant clone (Clone 1) of Mycobacterium abscessus subspecies massiliense (M. massiliense) associated with high prevalence in cystic fibrosis (CF) patients, pulmonary outbreaks in the United States (US) and United Kingdom (UK), and a Brazilian epidemic of skin infections. The prevalence of Clone 1 in non-CF patients in the US and the relationship of sporadic US isolates to outbreak clones are not known. We surveyed a reference US Mycobacteria Laboratory and a US biorepository of CF-associated Mycobacteria isolates for Clone 1. We then compared genomic variation and antimicrobial resistance (AMR) mutations between sporadic non-CF, CF, and outbreak Clone 1 isolates. Among reference lab samples, 57/147 (39%) of patients with M. massiliense had Clone 1, including pulmonary and extrapulmonary infections, compared to 11/64 (17%) in the CF isolate biorepository. Core and pan genome analyses revealed that outbreak isolates had similar numbers of single nucleotide polymorphisms (SNPs) and accessory genes as sporadic US Clone 1 isolates. However, pulmonary outbreak isolates were more likely to have AMR mutations compared to sporadic isolates. Clone 1 isolates are present among non-CF and CF patients across the US, but additional studies will be needed to resolve potential routes of transmission and spread.
format	article
author	Rebecca M. Davidson Jeanne B. Benoit Sara M. Kammlade Nabeeh A. Hasan L. Elaine Epperson Terry Smith Sruthi Vasireddy Barbara A. Brown-Elliott Jerry A. Nick Kenneth N. Olivier Adrian M. Zelazny Charles L. Daley Michael Strong Richard J. Wallace
author_facet	Rebecca M. Davidson Jeanne B. Benoit Sara M. Kammlade Nabeeh A. Hasan L. Elaine Epperson Terry Smith Sruthi Vasireddy Barbara A. Brown-Elliott Jerry A. Nick Kenneth N. Olivier Adrian M. Zelazny Charles L. Daley Michael Strong Richard J. Wallace
author_sort	Rebecca M. Davidson
title	Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
title_short	Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
title_full	Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
title_fullStr	Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
title_full_unstemmed	Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense
title_sort	genomic characterization of sporadic isolates of the dominant clone of mycobacterium abscessus subspecies massiliense
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65f02a743ac340e2baeb45d175365d25
work_keys_str_mv	AT rebeccamdavidson genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT jeannebbenoit genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT saramkammlade genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT nabeehahasan genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT lelaineepperson genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT terrysmith genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT sruthivasireddy genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT barbaraabrownelliott genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT jerryanick genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT kennethnolivier genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT adrianmzelazny genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT charlesldaley genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT michaelstrong genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense AT richardjwallace genomiccharacterizationofsporadicisolatesofthedominantcloneofmycobacteriumabscessussubspeciesmassiliense
_version_	1718383876733140992

Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense

Ejemplares similares