Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity

Ahmed S Zidan,1,2 Osama AA Ahmed,1,3 Bader M Aljaeid1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Zagazig University, Zagazig, 3Depar...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zidan AS, Ahmed OA, Aljaeid BM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nicotinamide polymeric nanoemulsified systems Placket-Burman design antibacterial activity sustained release. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65f1d23f159b49ed995bec5fedf69a9f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65f1d23f159b49ed995bec5fedf69a9f
record_format	dspace
spelling	oai:doaj.org-article:65f1d23f159b49ed995bec5fedf69a9f2021-12-02T02:08:31ZNicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity1178-2013https://doaj.org/article/65f1d23f159b49ed995bec5fedf69a9f2016-04-01T00:00:00Zhttps://www.dovepress.com/nicotinamide-polymeric-nanoemulsified-systems-a-quality-by-design-case-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ahmed S Zidan,1,2 Osama AA Ahmed,1,3 Bader M Aljaeid1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Zagazig University, Zagazig, 3Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Minia University, Minia, Egypt Abstract: Nicotinamide, the amide form of vitamin B3, was demonstrated to combat some of the antibiotic-resistant infections that are increasingly common around the world. The objective of this study was to thoroughly understand the formulation and process variabilities affecting the preparation of nicotinamide-loaded polymeric nanoemulsified particles. The quality target product profile and critical quality attributes of the proposed product were presented. Plackett–Burman screening design was employed to screen eight variables for their influences on the formulation’s critical characteristics. The formulations were prepared by an oil-in-water emulsification followed by solvent replacement. The prepared systems were characterized by entrapment capacity (EC), entrapment efficiency (EE), particle size, polydispersity index, zeta potential, transmission electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, powder X-ray diffraction, in vitro drug release, and their antibacterial activity against bacterial scrums. EC, EE, particle size, polydispersity index, zeta potential, and percentage release in 24 hours were found to be in the range of 33.5%–68.8%, 53.1%–67.1%, 43.3–243.3 nm, 0.08–0.28, 9.5–53.3 mV, and 5.8%–22.4%, respectively. One-way analysis of variance and Pareto charts revealed that the experimental loadings of 2-hydroxypropyl-β-cyclodextrin and Eudragit® S100 were the most significant for their effects on nicotinamide EC and EE. Moreover, the polymeric nanoemulsified particles demonstrated a sustained release profile for nicotinamide. The Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction demonstrated a significant interaction between the drug and 2-hydroxypropyl-β-cyclodextrin that might modulate the sustained release behavior. Furthermore, the formulations provided a sustained antibacterial activity that depended on nicotinamide-loading concentration, release rate, and incubation time. In conclusion, the study demonstrated the potential of polymeric nanoemulsified system to sustain the release and antibacterial activity of nicotinamide. Keywords: nicotinamide, polymeric nanoemulsified systems, Plackett–Burman design, antibacterial activity, sustained releaseZidan ASAhmed OAAljaeid BMDove Medical PressarticleNicotinamidepolymeric nanoemulsified systemsPlacket-Burman designantibacterial activitysustained release.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1501-1516 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nicotinamide polymeric nanoemulsified systems Placket-Burman design antibacterial activity sustained release. Medicine (General) R5-920
spellingShingle	Nicotinamide polymeric nanoemulsified systems Placket-Burman design antibacterial activity sustained release. Medicine (General) R5-920 Zidan AS Ahmed OA Aljaeid BM Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
description	Ahmed S Zidan,1,2 Osama AA Ahmed,1,3 Bader M Aljaeid1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Zagazig University, Zagazig, 3Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Minia University, Minia, Egypt Abstract: Nicotinamide, the amide form of vitamin B3, was demonstrated to combat some of the antibiotic-resistant infections that are increasingly common around the world. The objective of this study was to thoroughly understand the formulation and process variabilities affecting the preparation of nicotinamide-loaded polymeric nanoemulsified particles. The quality target product profile and critical quality attributes of the proposed product were presented. Plackett–Burman screening design was employed to screen eight variables for their influences on the formulation’s critical characteristics. The formulations were prepared by an oil-in-water emulsification followed by solvent replacement. The prepared systems were characterized by entrapment capacity (EC), entrapment efficiency (EE), particle size, polydispersity index, zeta potential, transmission electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, powder X-ray diffraction, in vitro drug release, and their antibacterial activity against bacterial scrums. EC, EE, particle size, polydispersity index, zeta potential, and percentage release in 24 hours were found to be in the range of 33.5%–68.8%, 53.1%–67.1%, 43.3–243.3 nm, 0.08–0.28, 9.5–53.3 mV, and 5.8%–22.4%, respectively. One-way analysis of variance and Pareto charts revealed that the experimental loadings of 2-hydroxypropyl-β-cyclodextrin and Eudragit® S100 were the most significant for their effects on nicotinamide EC and EE. Moreover, the polymeric nanoemulsified particles demonstrated a sustained release profile for nicotinamide. The Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction demonstrated a significant interaction between the drug and 2-hydroxypropyl-β-cyclodextrin that might modulate the sustained release behavior. Furthermore, the formulations provided a sustained antibacterial activity that depended on nicotinamide-loading concentration, release rate, and incubation time. In conclusion, the study demonstrated the potential of polymeric nanoemulsified system to sustain the release and antibacterial activity of nicotinamide. Keywords: nicotinamide, polymeric nanoemulsified systems, Plackett–Burman design, antibacterial activity, sustained release
format	article
author	Zidan AS Ahmed OA Aljaeid BM
author_facet	Zidan AS Ahmed OA Aljaeid BM
author_sort	Zidan AS
title	Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
title_short	Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
title_full	Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
title_fullStr	Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
title_full_unstemmed	Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
title_sort	nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/65f1d23f159b49ed995bec5fedf69a9f
work_keys_str_mv	AT zidanas nicotinamidepolymericnanoemulsifiedsystemsaqualitybydesigncasestudyforasustainedantimicrobialactivity AT ahmedoa nicotinamidepolymericnanoemulsifiedsystemsaqualitybydesigncasestudyforasustainedantimicrobialactivity AT aljaeidbm nicotinamidepolymericnanoemulsifiedsystemsaqualitybydesigncasestudyforasustainedantimicrobialactivity
_version_	1718402652263415808

Nicotinamide polymeric nanoemulsified systems: a quality-by-design case study for a sustained antimicrobial activity

Ejemplares similares