A biomimetic nanoparticle-enabled toxoid vaccine against melittin

 Tianyi Kang,1 Chenyang Li,1,2 Ting Du,1 Yujiao Wu,1 Yuping Yang,1 Xuan Liu,1 Qianqian Zhang,1 Xiaoping Xu,3 Maling Gou1,4 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 2Shanghai Institu...

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Autores principales:	Kang T, Li C, Du T, Wu Y, Yang Y, Liu X, Zhang Q, Xu X, Gou M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	PDA nanoparticles Melittin Toxoid Vaccine Immunity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/65f8694c6d874dc59e11f0ba94b896be
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spelling	oai:doaj.org-article:65f8694c6d874dc59e11f0ba94b896be2021-12-02T09:02:47ZA biomimetic nanoparticle-enabled toxoid vaccine against melittin1178-2013https://doaj.org/article/65f8694c6d874dc59e11f0ba94b896be2018-06-01T00:00:00Zhttps://www.dovepress.com/a-biomimetic-nanoparticle-enabled-toxoid-vaccine-against-melittin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Tianyi Kang,1 Chenyang Li,1,2 Ting Du,1 Yujiao Wu,1 Yuping Yang,1 Xuan Liu,1 Qianqian Zhang,1 Xiaoping Xu,3 Maling Gou1,4 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 2Shanghai Institutes for Biological Science, Chinese Academy of Science, Shanghai 200031, People’s Republic of China; 3West China School of Pharmacy, Sichuan University, Chengdu 610041, People’s Republic of China; 4Co-Innovation Center for Micro/Nano Optoelectronic Materials and Devices, Chongqing 402160, People’s Republic of China Background: Melittin, the main active peptide ingredient of bee venom, can cause severe cell membrane lysis due to its robust interaction with negatively charged phospholipids. So far, no effective anti-melittin vaccine has been developed to protect people from undesired melittin intoxication.Methods: Herein, we prepared a polydiacetylene (PDA) nanoparticle with cell membrane-mimic surface to complex melittin, forming an anti-melittin vaccine (PDA–melittin).Results: PDA nanoparticles could effectively combine with melittin and neutralize its toxicity. PDA–melittin nanocomplex is demonstrated to enhance melittin uptake by DCs and stimulate strong melittin-specific immunity. Mice immunized with PDA–melittin nanocomplex showed higher survival rate after exposion to melittin than untreated mice.Conclusion: The PDA–melittin nanocomplex can efficiently and safely generate a specific immunity against melittin to protect body from melittin intoxication, providing a new method with potential clinical application for the treatment of melittin intoxication.Keywords: PDA nanoparticles, melittin, toxoid vaccine, immunityKang TLi CDu TWu YYang YLiu XZhang QXu XGou MDove Medical PressarticlePDA nanoparticlesMelittinToxoid VaccineImmunityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3251-3261 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	PDA nanoparticles Melittin Toxoid Vaccine Immunity Medicine (General) R5-920
spellingShingle	PDA nanoparticles Melittin Toxoid Vaccine Immunity Medicine (General) R5-920 Kang T Li C Du T Wu Y Yang Y Liu X Zhang Q Xu X Gou M A biomimetic nanoparticle-enabled toxoid vaccine against melittin
description	Tianyi Kang,1 Chenyang Li,1,2 Ting Du,1 Yujiao Wu,1 Yuping Yang,1 Xuan Liu,1 Qianqian Zhang,1 Xiaoping Xu,3 Maling Gou1,4 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 2Shanghai Institutes for Biological Science, Chinese Academy of Science, Shanghai 200031, People’s Republic of China; 3West China School of Pharmacy, Sichuan University, Chengdu 610041, People’s Republic of China; 4Co-Innovation Center for Micro/Nano Optoelectronic Materials and Devices, Chongqing 402160, People’s Republic of China Background: Melittin, the main active peptide ingredient of bee venom, can cause severe cell membrane lysis due to its robust interaction with negatively charged phospholipids. So far, no effective anti-melittin vaccine has been developed to protect people from undesired melittin intoxication.Methods: Herein, we prepared a polydiacetylene (PDA) nanoparticle with cell membrane-mimic surface to complex melittin, forming an anti-melittin vaccine (PDA–melittin).Results: PDA nanoparticles could effectively combine with melittin and neutralize its toxicity. PDA–melittin nanocomplex is demonstrated to enhance melittin uptake by DCs and stimulate strong melittin-specific immunity. Mice immunized with PDA–melittin nanocomplex showed higher survival rate after exposion to melittin than untreated mice.Conclusion: The PDA–melittin nanocomplex can efficiently and safely generate a specific immunity against melittin to protect body from melittin intoxication, providing a new method with potential clinical application for the treatment of melittin intoxication.Keywords: PDA nanoparticles, melittin, toxoid vaccine, immunity
format	article
author	Kang T Li C Du T Wu Y Yang Y Liu X Zhang Q Xu X Gou M
author_facet	Kang T Li C Du T Wu Y Yang Y Liu X Zhang Q Xu X Gou M
author_sort	Kang T
title	A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_short	A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_full	A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_fullStr	A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_full_unstemmed	A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_sort	biomimetic nanoparticle-enabled toxoid vaccine against melittin
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/65f8694c6d874dc59e11f0ba94b896be
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_version_	1718398247653867520

A biomimetic nanoparticle-enabled toxoid vaccine against melittin

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