Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.

Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. T...

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Autores principales:	Mingfeng Li, Hongxing Sun, Qiong Feng, Huiming Lu, Ye Zhao, Hui Zhang, Xin Xu, Jiandong Jiao, Liangyan Wang, Yuejin Hua
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65fa94d4c5994c76a52ef80a31dcc2fb
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spelling	oai:doaj.org-article:65fa94d4c5994c76a52ef80a31dcc2fb2021-11-18T08:00:02ZExtracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.1932-620310.1371/journal.pone.0054420https://doaj.org/article/65fa94d4c5994c76a52ef80a31dcc2fb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23365666/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. The results showed that the extracellular DNA fragments inhibited the growth of D. radiodurans. Interestingly, dGMP, a DNA component, enhanced D. radiodurans tolerance to H(2)O(2) and gamma-radiation significantly. Further experiments indicated that extracellular dGMP stimulated the activity of one catalase (KatA, DR1998), and induced gene transcription including the extracellular nuclease (drb0067). When this only extracellular nuclease gene (drb0067) in D. radiodurans was deleted, the mutant strain showed more sensitive to H(2)O(2) and gamma-radiation than the wild type strain. These results suggest that DRB0067 plays an important role in oxidative stress resistance. Taken together, we proposed a new anti-oxidation mechanism in D. radiodurans. This mechanism acts to increase expression levels of DRB0067 which then secretes active nuclease to degrade extracellular DNA fragments. The extracellular nuclease has a two-fold benefit, creating more free dNTPs for further cell protection and the removal of extracellular DNA fragments.Mingfeng LiHongxing SunQiong FengHuiming LuYe ZhaoHui ZhangXin XuJiandong JiaoLiangyan WangYuejin HuaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54420 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mingfeng Li Hongxing Sun Qiong Feng Huiming Lu Ye Zhao Hui Zhang Xin Xu Jiandong Jiao Liangyan Wang Yuejin Hua Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
description	Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. The results showed that the extracellular DNA fragments inhibited the growth of D. radiodurans. Interestingly, dGMP, a DNA component, enhanced D. radiodurans tolerance to H(2)O(2) and gamma-radiation significantly. Further experiments indicated that extracellular dGMP stimulated the activity of one catalase (KatA, DR1998), and induced gene transcription including the extracellular nuclease (drb0067). When this only extracellular nuclease gene (drb0067) in D. radiodurans was deleted, the mutant strain showed more sensitive to H(2)O(2) and gamma-radiation than the wild type strain. These results suggest that DRB0067 plays an important role in oxidative stress resistance. Taken together, we proposed a new anti-oxidation mechanism in D. radiodurans. This mechanism acts to increase expression levels of DRB0067 which then secretes active nuclease to degrade extracellular DNA fragments. The extracellular nuclease has a two-fold benefit, creating more free dNTPs for further cell protection and the removal of extracellular DNA fragments.
format	article
author	Mingfeng Li Hongxing Sun Qiong Feng Huiming Lu Ye Zhao Hui Zhang Xin Xu Jiandong Jiao Liangyan Wang Yuejin Hua
author_facet	Mingfeng Li Hongxing Sun Qiong Feng Huiming Lu Ye Zhao Hui Zhang Xin Xu Jiandong Jiao Liangyan Wang Yuejin Hua
author_sort	Mingfeng Li
title	Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
title_short	Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
title_full	Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
title_fullStr	Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
title_full_unstemmed	Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.
title_sort	extracellular dgmp enhances deinococcus radiodurans tolerance to oxidative stress.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/65fa94d4c5994c76a52ef80a31dcc2fb
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Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.

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