Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells

Abstract Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic reso...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simon R. Hemelaar, Kiran J. van der Laan, Sophie R. Hinterding, Manon V. Koot, Else Ellermann, Felipe P. Perona-Martinez, David Roig, Severin Hommelet, Daniele Novarina, Hiroki Takahashi, Michael Chang, Romana Schirhagl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65fb70e9334f4edfbb9c390f9d3a23db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:65fb70e9334f4edfbb9c390f9d3a23db
record_format	dspace
spelling	oai:doaj.org-article:65fb70e9334f4edfbb9c390f9d3a23db2021-12-02T11:53:10ZGenerally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells10.1038/s41598-017-06180-52045-2322https://doaj.org/article/65fb70e9334f4edfbb9c390f9d3a23db2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06180-5https://doaj.org/toc/2045-2322Abstract Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5% DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.Simon R. HemelaarKiran J. van der LaanSophie R. HinterdingManon V. KootElse EllermannFelipe P. Perona-MartinezDavid RoigSeverin HommeletDaniele NovarinaHiroki TakahashiMichael ChangRomana SchirhaglNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon R. Hemelaar Kiran J. van der Laan Sophie R. Hinterding Manon V. Koot Else Ellermann Felipe P. Perona-Martinez David Roig Severin Hommelet Daniele Novarina Hiroki Takahashi Michael Chang Romana Schirhagl Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
description	Abstract Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5% DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.
format	article
author	Simon R. Hemelaar Kiran J. van der Laan Sophie R. Hinterding Manon V. Koot Else Ellermann Felipe P. Perona-Martinez David Roig Severin Hommelet Daniele Novarina Hiroki Takahashi Michael Chang Romana Schirhagl
author_facet	Simon R. Hemelaar Kiran J. van der Laan Sophie R. Hinterding Manon V. Koot Else Ellermann Felipe P. Perona-Martinez David Roig Severin Hommelet Daniele Novarina Hiroki Takahashi Michael Chang Romana Schirhagl
author_sort	Simon R. Hemelaar
title	Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
title_short	Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
title_full	Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
title_fullStr	Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
title_full_unstemmed	Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
title_sort	generally applicable transformation protocols for fluorescent nanodiamond internalization into cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/65fb70e9334f4edfbb9c390f9d3a23db
work_keys_str_mv	AT simonrhemelaar generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT kiranjvanderlaan generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT sophierhinterding generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT manonvkoot generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT elseellermann generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT felipepperonamartinez generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT davidroig generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT severinhommelet generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT danielenovarina generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT hirokitakahashi generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT michaelchang generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells AT romanaschirhagl generallyapplicabletransformationprotocolsforfluorescentnanodiamondinternalizationintocells
_version_	1718394870013362176

Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells

Ejemplares similares