Protective effect of TM6 on LPS-induced acute lung injury in mice

Abstract Acute lung injury (ALI) is an acute failure of the respiratory system for which effective treatment is urgently necessary. Previous studies found that several peptides potently inhibited the production of cytokines induced by lipopolysaccharide (LPS). In this study, we synthetized a cell-pe...

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Autores principales:	Xiaoyu Hu, Yuan Tian, Shihui Qu, Yongguo Cao, Shumin Li, Wenlong Zhang, Zecai Zhang, Naisheng Zhang, Yunhe Fu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65fbb1a0f78b465588c2b87c22d571ed
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spelling	oai:doaj.org-article:65fbb1a0f78b465588c2b87c22d571ed2021-12-02T11:52:30ZProtective effect of TM6 on LPS-induced acute lung injury in mice10.1038/s41598-017-00551-82045-2322https://doaj.org/article/65fbb1a0f78b465588c2b87c22d571ed2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00551-8https://doaj.org/toc/2045-2322Abstract Acute lung injury (ALI) is an acute failure of the respiratory system for which effective treatment is urgently necessary. Previous studies found that several peptides potently inhibited the production of cytokines induced by lipopolysaccharide (LPS). In this study, we synthetized a cell-permeable TIR domain-derived decoy peptide (TM6) and examined its substance for the ability to inhibit TLR signaling in the model of ALI induced by LPS. We demonstrated that TM6 (2.5, 5 and 10 nmol/g) alleviated the histological changes in the lung tissues as well as myeloperoxtidase (MPO) activity, lung W/D ratio, the production of TNF-α, IL-1β and IL-6 induced by LPS. Furthermore, the numbers of total cells, neutrophils and macrophages in the BALF were suppressed by TM6. In vitro, TM6 (5, 10 and 20 µM) inhibited the production of TNF-α, IL-1β and IL-6 in LPS-stimulated alveolar macrophages. Moreover, the activation of Nuclear factor-kappaB (NF-κB) and Mitogen activated protein kinases (MAPK) signaling pathways induced by LPS were also inhibited by TM6. Collectively, our results suggested that TM6 was an effective inhibitor of ALI induced by LPS, and this peptide may very well serve as a future treatment for ALI.Xiaoyu HuYuan TianShihui QuYongguo CaoShumin LiWenlong ZhangZecai ZhangNaisheng ZhangYunhe FuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoyu Hu Yuan Tian Shihui Qu Yongguo Cao Shumin Li Wenlong Zhang Zecai Zhang Naisheng Zhang Yunhe Fu Protective effect of TM6 on LPS-induced acute lung injury in mice
description	Abstract Acute lung injury (ALI) is an acute failure of the respiratory system for which effective treatment is urgently necessary. Previous studies found that several peptides potently inhibited the production of cytokines induced by lipopolysaccharide (LPS). In this study, we synthetized a cell-permeable TIR domain-derived decoy peptide (TM6) and examined its substance for the ability to inhibit TLR signaling in the model of ALI induced by LPS. We demonstrated that TM6 (2.5, 5 and 10 nmol/g) alleviated the histological changes in the lung tissues as well as myeloperoxtidase (MPO) activity, lung W/D ratio, the production of TNF-α, IL-1β and IL-6 induced by LPS. Furthermore, the numbers of total cells, neutrophils and macrophages in the BALF were suppressed by TM6. In vitro, TM6 (5, 10 and 20 µM) inhibited the production of TNF-α, IL-1β and IL-6 in LPS-stimulated alveolar macrophages. Moreover, the activation of Nuclear factor-kappaB (NF-κB) and Mitogen activated protein kinases (MAPK) signaling pathways induced by LPS were also inhibited by TM6. Collectively, our results suggested that TM6 was an effective inhibitor of ALI induced by LPS, and this peptide may very well serve as a future treatment for ALI.
format	article
author	Xiaoyu Hu Yuan Tian Shihui Qu Yongguo Cao Shumin Li Wenlong Zhang Zecai Zhang Naisheng Zhang Yunhe Fu
author_facet	Xiaoyu Hu Yuan Tian Shihui Qu Yongguo Cao Shumin Li Wenlong Zhang Zecai Zhang Naisheng Zhang Yunhe Fu
author_sort	Xiaoyu Hu
title	Protective effect of TM6 on LPS-induced acute lung injury in mice
title_short	Protective effect of TM6 on LPS-induced acute lung injury in mice
title_full	Protective effect of TM6 on LPS-induced acute lung injury in mice
title_fullStr	Protective effect of TM6 on LPS-induced acute lung injury in mice
title_full_unstemmed	Protective effect of TM6 on LPS-induced acute lung injury in mice
title_sort	protective effect of tm6 on lps-induced acute lung injury in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/65fbb1a0f78b465588c2b87c22d571ed
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Protective effect of TM6 on LPS-induced acute lung injury in mice

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