Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium

Abstract Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified...

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Autores principales:	Adam J. Replogle, Christopher Sexton, John Young, Luke C. Kingry, Martin E. Schriefer, Marc Dolan, Tammi L. Johnson, Neeta P. Connally, Kerry A. Padgett, Jeannine M. Petersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65fd9b5035ef46768e74342060f79717
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spelling	oai:doaj.org-article:65fd9b5035ef46768e74342060f797172021-12-02T15:23:28ZIsolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium10.1038/s41598-021-81252-12045-2322https://doaj.org/article/65fd9b5035ef46768e74342060f797172021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81252-1https://doaj.org/toc/2045-2322Abstract Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified Barbour-Stoenner-Kelly (BSK) media formulation, BSK-R, was developed for isolation of the emerging RF pathogen, Borrelia miyamotoi. BSK-R is a diluted BSK-II derivative supplemented with Lebovitz’s L-15, mouse and fetal calf serum. Decreasing the concentration of CMRL 1066 and other components was essential for growth of North American B. miyamotoi. Sixteen B. miyamotoi isolates, originating from Ixodes scapularis ticks, rodent and human blood collected in the eastern and upper midwestern United States, were isolated and propagated to densities > 108 spirochetes/mL. Growth of five other RF and ten different LB borreliae readily occurred in BSK-R. Additionally, primary culture recovery of 20 isolates of Borrelia hermsii, Borrelia turicatae, Borrelia burgdorferi and Borrelia mayonii was achieved in BSK-R using whole blood from infected patients. These data indicate this broadly encompassing borreliae media can aid in in vitro culture recovery of RF and LB spirochetes, including the direct isolation of new and emerging human pathogens.Adam J. ReplogleChristopher SextonJohn YoungLuke C. KingryMartin E. SchrieferMarc DolanTammi L. JohnsonNeeta P. ConnallyKerry A. PadgettJeannine M. PetersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adam J. Replogle Christopher Sexton John Young Luke C. Kingry Martin E. Schriefer Marc Dolan Tammi L. Johnson Neeta P. Connally Kerry A. Padgett Jeannine M. Petersen Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
description	Abstract Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified Barbour-Stoenner-Kelly (BSK) media formulation, BSK-R, was developed for isolation of the emerging RF pathogen, Borrelia miyamotoi. BSK-R is a diluted BSK-II derivative supplemented with Lebovitz’s L-15, mouse and fetal calf serum. Decreasing the concentration of CMRL 1066 and other components was essential for growth of North American B. miyamotoi. Sixteen B. miyamotoi isolates, originating from Ixodes scapularis ticks, rodent and human blood collected in the eastern and upper midwestern United States, were isolated and propagated to densities > 108 spirochetes/mL. Growth of five other RF and ten different LB borreliae readily occurred in BSK-R. Additionally, primary culture recovery of 20 isolates of Borrelia hermsii, Borrelia turicatae, Borrelia burgdorferi and Borrelia mayonii was achieved in BSK-R using whole blood from infected patients. These data indicate this broadly encompassing borreliae media can aid in in vitro culture recovery of RF and LB spirochetes, including the direct isolation of new and emerging human pathogens.
format	article
author	Adam J. Replogle Christopher Sexton John Young Luke C. Kingry Martin E. Schriefer Marc Dolan Tammi L. Johnson Neeta P. Connally Kerry A. Padgett Jeannine M. Petersen
author_facet	Adam J. Replogle Christopher Sexton John Young Luke C. Kingry Martin E. Schriefer Marc Dolan Tammi L. Johnson Neeta P. Connally Kerry A. Padgett Jeannine M. Petersen
author_sort	Adam J. Replogle
title	Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
title_short	Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
title_full	Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
title_fullStr	Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
title_full_unstemmed	Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
title_sort	isolation of borrelia miyamotoi and other borreliae using a modified bsk medium
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65fd9b5035ef46768e74342060f79717
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Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium

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