Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium

Background: The isolation of cellulolytic bacteria, which hydrolyze cellulose to cellobiose and glucose, can provide useful information about rumen diversity. Objective: To identify and characterize a microorganism capable of hydrolyzing cellulose, isolated from a cow rumen. Methods: Anaerobic cultu...

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Autores principales:	Lorena Luna, David Hernández, Hilda V. Silva, Mario A. Cobos, Sergio S. González, César Cortez, René Pinto, Efrén Ramírez, Juan M. Pinos, Juan M. Vargas
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	cellulolytic fermentation monophyletic rumen shigella Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/65feadfef16e4b6d9e5ec8590fe4b69a
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spelling	oai:doaj.org-article:65feadfef16e4b6d9e5ec8590fe4b69a2021-12-01T18:59:21ZIsolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium2256-295810.17533/udea.rccp.v32n2a05https://doaj.org/article/65feadfef16e4b6d9e5ec8590fe4b69a2019-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/335056https://doaj.org/toc/2256-2958Background: The isolation of cellulolytic bacteria, which hydrolyze cellulose to cellobiose and glucose, can provide useful information about rumen diversity. Objective: To identify and characterize a microorganism capable of hydrolyzing cellulose, isolated from a cow rumen. Methods: Anaerobic culture techniques were used for isolating cellulose-degrading rumen bacteria. Congo red staining was used to evaluate β-D-glucanase activity, and carbohydrate fermentation pattern was obtained with the kit API 50CHB/E. DNA extraction was performed and the 16S rDNA gene was amplified using 8F (5'-AGA GTT TGA TCC TGG CTC AG-3'), and 1492R (5' GGT TAC CTT GTT ACG ACT T 3') primers. The phylogenetic tree was reconstructed with the algorithm of maximum parsimony (bootstrap 5000), and 16S rDNA sequence was deposited in the NCBI database (accession number: KM094184). Results: The isolated bacterium showed cellulolytic activity detected with Congo red; besides, glycerol, ribose, xylose, sucrose, galactose and glucose were fermented by this bacterium. However, biochemical tests did not identify the bacteria because no match was found at database of API WEB Software. The phylogenetic inference indicated that this bacterium belongs to Shigella genus, with 98% maximal identity respect to the other taxonomic species. Conclusions: Phylogenetic analysis of 16S rRNA genes showed that the rumen isolated bacterium was a member of the genus Shigella, which, under mesophilic conditions, is an interesting candidate for obtaining oligosaccharides from lignocellulosic biomass.Lorena LunaDavid HernándezHilda V. SilvaMario A. CobosSergio S. GonzálezCésar CortezRené PintoEfrén RamírezJuan M. PinosJuan M. VargasUniversidad de AntioquiaarticlecellulolyticfermentationmonophyleticrumenshigellaAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 2, Pp 117-125 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulolytic fermentation monophyletic rumen shigella Animal culture SF1-1100
spellingShingle	cellulolytic fermentation monophyletic rumen shigella Animal culture SF1-1100 Lorena Luna David Hernández Hilda V. Silva Mario A. Cobos Sergio S. González César Cortez René Pinto Efrén Ramírez Juan M. Pinos Juan M. Vargas Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
description	Background: The isolation of cellulolytic bacteria, which hydrolyze cellulose to cellobiose and glucose, can provide useful information about rumen diversity. Objective: To identify and characterize a microorganism capable of hydrolyzing cellulose, isolated from a cow rumen. Methods: Anaerobic culture techniques were used for isolating cellulose-degrading rumen bacteria. Congo red staining was used to evaluate β-D-glucanase activity, and carbohydrate fermentation pattern was obtained with the kit API 50CHB/E. DNA extraction was performed and the 16S rDNA gene was amplified using 8F (5'-AGA GTT TGA TCC TGG CTC AG-3'), and 1492R (5' GGT TAC CTT GTT ACG ACT T 3') primers. The phylogenetic tree was reconstructed with the algorithm of maximum parsimony (bootstrap 5000), and 16S rDNA sequence was deposited in the NCBI database (accession number: KM094184). Results: The isolated bacterium showed cellulolytic activity detected with Congo red; besides, glycerol, ribose, xylose, sucrose, galactose and glucose were fermented by this bacterium. However, biochemical tests did not identify the bacteria because no match was found at database of API WEB Software. The phylogenetic inference indicated that this bacterium belongs to Shigella genus, with 98% maximal identity respect to the other taxonomic species. Conclusions: Phylogenetic analysis of 16S rRNA genes showed that the rumen isolated bacterium was a member of the genus Shigella, which, under mesophilic conditions, is an interesting candidate for obtaining oligosaccharides from lignocellulosic biomass.
format	article
author	Lorena Luna David Hernández Hilda V. Silva Mario A. Cobos Sergio S. González César Cortez René Pinto Efrén Ramírez Juan M. Pinos Juan M. Vargas
author_facet	Lorena Luna David Hernández Hilda V. Silva Mario A. Cobos Sergio S. González César Cortez René Pinto Efrén Ramírez Juan M. Pinos Juan M. Vargas
author_sort	Lorena Luna
title	Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
title_short	Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
title_full	Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
title_fullStr	Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
title_full_unstemmed	Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
title_sort	isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/65feadfef16e4b6d9e5ec8590fe4b69a
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Isolation, biochemical characterization and phylogeny of a cellulose-degrading ruminal bacterium

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