Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice

Abstract We investigated the impact of aerobic exercise (AE) on multiple organ dysfunction syndrome (MODS), aortic injury, pathoglycemia, and death during sepsis. ICR mice were randomized into four groups: Control (Con), Lipopolysaccharide (LPS), Exercise (Ex), and Exercise + LPS (Ex + LPS) groups....

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Autores principales:	Xishuai Wang, Zhiqing Wang, Donghui Tang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/65ff7e2895444612acc286b7e582aa91
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spelling	oai:doaj.org-article:65ff7e2895444612acc286b7e582aa912021-12-02T17:19:16ZAerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice10.1038/s41598-021-97101-02045-2322https://doaj.org/article/65ff7e2895444612acc286b7e582aa912021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97101-0https://doaj.org/toc/2045-2322Abstract We investigated the impact of aerobic exercise (AE) on multiple organ dysfunction syndrome (MODS), aortic injury, pathoglycemia, and death during sepsis. ICR mice were randomized into four groups: Control (Con), Lipopolysaccharide (LPS), Exercise (Ex), and Exercise + LPS (Ex + LPS) groups. Mice were trained with low-intensity for 4 weeks. LPS and Ex + LPS mice received 5 mg/kg LPS intraperitoneally for induction of sepsis. Histopathological micrographs showed the organ morphology and damage. This study examined the effects of AE on LPS-induced changes in systemic inflammation, pulmonary inflammation, lung permeability, and bronchoalveolar lavage fluid (BALF) cell count, oxidative stress-related indicators in the lung, blood glucose levels, plasma lactate levels, serum insulin levels, plasma high-mobility group box 1 (HMGB1) levels, glucose transporter 1 (Glut1) and HMGB1, silent information regulator 1 (Sirt-1), and nuclear factor erythroid 2-related factor 2 (Nrf-2) mRNA expression levels in lung tissue. AE improved sepsis-associated multiple organ dysfunction syndrome (MODS), aortic injury, hypoglycemia, and death. AE prominently decreased pulmonary inflammation, pulmonary edema, and modulated redox balance during sepsis. AE prominently decreased neutrophil content in organ. AE prominently downregulated CXCL-1, CXCL-8, IL-6, TNF-α, Glu1, and HMGB1 mRNA expression but activated IL-1RN, IL-10, Sirt-1, and Nrf-2 mRNA expression in the lung during sepsis. AE decreased the serum levels of lactate and HMGB1 but increased blood glucose levels and serum insulin levels during sepsis. A 4-week AE improves sepsis-associated MODS, aortic injury, pathoglycemia, and death. AE impairs LPS-induced lactate and HMGB1 release partly because AE increases serum insulin levels and decreases the levels of Glut1. AE is a novel therapeutic strategy for sepsis targeting aerobic glycolysis.Xishuai WangZhiqing WangDonghui TangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xishuai Wang Zhiqing Wang Donghui Tang Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
description	Abstract We investigated the impact of aerobic exercise (AE) on multiple organ dysfunction syndrome (MODS), aortic injury, pathoglycemia, and death during sepsis. ICR mice were randomized into four groups: Control (Con), Lipopolysaccharide (LPS), Exercise (Ex), and Exercise + LPS (Ex + LPS) groups. Mice were trained with low-intensity for 4 weeks. LPS and Ex + LPS mice received 5 mg/kg LPS intraperitoneally for induction of sepsis. Histopathological micrographs showed the organ morphology and damage. This study examined the effects of AE on LPS-induced changes in systemic inflammation, pulmonary inflammation, lung permeability, and bronchoalveolar lavage fluid (BALF) cell count, oxidative stress-related indicators in the lung, blood glucose levels, plasma lactate levels, serum insulin levels, plasma high-mobility group box 1 (HMGB1) levels, glucose transporter 1 (Glut1) and HMGB1, silent information regulator 1 (Sirt-1), and nuclear factor erythroid 2-related factor 2 (Nrf-2) mRNA expression levels in lung tissue. AE improved sepsis-associated multiple organ dysfunction syndrome (MODS), aortic injury, hypoglycemia, and death. AE prominently decreased pulmonary inflammation, pulmonary edema, and modulated redox balance during sepsis. AE prominently decreased neutrophil content in organ. AE prominently downregulated CXCL-1, CXCL-8, IL-6, TNF-α, Glu1, and HMGB1 mRNA expression but activated IL-1RN, IL-10, Sirt-1, and Nrf-2 mRNA expression in the lung during sepsis. AE decreased the serum levels of lactate and HMGB1 but increased blood glucose levels and serum insulin levels during sepsis. A 4-week AE improves sepsis-associated MODS, aortic injury, pathoglycemia, and death. AE impairs LPS-induced lactate and HMGB1 release partly because AE increases serum insulin levels and decreases the levels of Glut1. AE is a novel therapeutic strategy for sepsis targeting aerobic glycolysis.
format	article
author	Xishuai Wang Zhiqing Wang Donghui Tang
author_facet	Xishuai Wang Zhiqing Wang Donghui Tang
author_sort	Xishuai Wang
title	Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
title_short	Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
title_full	Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
title_fullStr	Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
title_full_unstemmed	Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice
title_sort	aerobic exercise improves lps-induced sepsis via regulating the warburg effect in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/65ff7e2895444612acc286b7e582aa91
work_keys_str_mv	AT xishuaiwang aerobicexerciseimproveslpsinducedsepsisviaregulatingthewarburgeffectinmice AT zhiqingwang aerobicexerciseimproveslpsinducedsepsisviaregulatingthewarburgeffectinmice AT donghuitang aerobicexerciseimproveslpsinducedsepsisviaregulatingthewarburgeffectinmice
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Aerobic exercise improves LPS-induced sepsis via regulating the Warburg effect in mice

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