A peptide-mediated targeting gene delivery system for malignant glioma cells

Chuanwei Wang,1,2,* Liping Ning,3,* Hongwei Wang,1,2,* Zaijun Lu,4 Xingang Li,1,2 Xiaoyong Fan,5 Xuping Wang,6 Yuguang Liu1,2 1Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People's Republic of China; 2Brain Science Research Institute of Shandong University, Jinan,...

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Autores principales:	Wang C, Ning L, Wang H, Lu Z, Li X, Fan X, Wang X, Liu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/660137a1c5d7496193d3c0a084a518dd
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spelling	oai:doaj.org-article:660137a1c5d7496193d3c0a084a518dd2021-12-02T07:23:00ZA peptide-mediated targeting gene delivery system for malignant glioma cells1176-91141178-2013https://doaj.org/article/660137a1c5d7496193d3c0a084a518dd2013-09-01T00:00:00Zhttp://www.dovepress.com/a-peptide-mediated-targeting-gene-delivery-system-for-malignant-glioma-a14455https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chuanwei Wang,1,2,* Liping Ning,3,* Hongwei Wang,1,2,* Zaijun Lu,4 Xingang Li,1,2 Xiaoyong Fan,5 Xuping Wang,6 Yuguang Liu1,2 1Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People's Republic of China; 2Brain Science Research Institute of Shandong University, Jinan, People's Republic of China; 3Department of Rehabilitation, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China; 4School of Chemistry and Chemical Engineering of Shandong University, Jinan, People's Republic of China; 5Department of Neurosurgery, Shandong Qianfoshan Hospital Affiliated to Shandong University, Jinan, People's Republic of China; 6Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital of Shandong University, Jinan, People's Republic of China *These authors contributed equally to this work Abstract: Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner. Keywords: glioblastoma, polyethylenimine, nanoparticles, drug-delivery systems, gene transfer techniquesWang CNing LWang HLu ZLi XFan XWang XLiu YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3631-3640 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang C Ning L Wang H Lu Z Li X Fan X Wang X Liu Y A peptide-mediated targeting gene delivery system for malignant glioma cells
description	Chuanwei Wang,1,2,* Liping Ning,3,* Hongwei Wang,1,2,* Zaijun Lu,4 Xingang Li,1,2 Xiaoyong Fan,5 Xuping Wang,6 Yuguang Liu1,2 1Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People's Republic of China; 2Brain Science Research Institute of Shandong University, Jinan, People's Republic of China; 3Department of Rehabilitation, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China; 4School of Chemistry and Chemical Engineering of Shandong University, Jinan, People's Republic of China; 5Department of Neurosurgery, Shandong Qianfoshan Hospital Affiliated to Shandong University, Jinan, People's Republic of China; 6Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital of Shandong University, Jinan, People's Republic of China *These authors contributed equally to this work Abstract: Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner. Keywords: glioblastoma, polyethylenimine, nanoparticles, drug-delivery systems, gene transfer techniques
format	article
author	Wang C Ning L Wang H Lu Z Li X Fan X Wang X Liu Y
author_facet	Wang C Ning L Wang H Lu Z Li X Fan X Wang X Liu Y
author_sort	Wang C
title	A peptide-mediated targeting gene delivery system for malignant glioma cells
title_short	A peptide-mediated targeting gene delivery system for malignant glioma cells
title_full	A peptide-mediated targeting gene delivery system for malignant glioma cells
title_fullStr	A peptide-mediated targeting gene delivery system for malignant glioma cells
title_full_unstemmed	A peptide-mediated targeting gene delivery system for malignant glioma cells
title_sort	peptide-mediated targeting gene delivery system for malignant glioma cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/660137a1c5d7496193d3c0a084a518dd
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A peptide-mediated targeting gene delivery system for malignant glioma cells

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