Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.

Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of different temperatures on mycelial growth of these...

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Autores principales:	Amgad A Saleh, Anwar H Sharafaddin, Mahmoud H El Komy, Yasser E Ibrahim, Younis K Hamad
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6607a16276ea449fa43c4c8840c48da8
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spelling	oai:doaj.org-article:6607a16276ea449fa43c4c8840c48da82021-12-02T20:06:35ZMolecular and physiological characterization of Fusarium strains associated with different diseases in date palm.1932-620310.1371/journal.pone.0254170https://doaj.org/article/6607a16276ea449fa43c4c8840c48da82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254170https://doaj.org/toc/1932-6203Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of different temperatures on mycelial growth of these strains. The pathogenicity of four strains of F. proliferatum was also evaluated on local date palm cultivars. Eleven SSR markers amplified a total of 57 scorable alleles from Fusarium strains. Phylogenetic analysis showed that F. proliferatum strains grouped in one clade with 95% bootstrap value. Within F. proliferatum clade, 14 SSR genotypes were identified, 9 of them were singleton. Four out of the five multi-individual SSR genotypes contained strains isolated from more than one location. Most F. solani strains grouped in one clade with 95% bootstrap value. Overall, the SSR markers previously developed for F. verticillioides and F. oxysporum were very useful in assessing the genetic diversity and confirming the identity of Saudi Fusarium strains. The results from the temperature study showed significant differences in mycelial growth of Fusarium strains at different temperatures tested. The highest average radial growth for Fusarium strains was observed at 25°C, irrespective of species. The four F. proliferatum strains showed significant differences in their pathogenicity on date palm cultivars. It is anticipated that the assessment of genetic diversity, effect of temperature on hyphal growth and pathogenicity of potent pathogenic Fusarium strains recovered from date palm-growing locations in Saudi Arabia can help in effectively controlling these pathogens.Amgad A SalehAnwar H SharafaddinMahmoud H El KomyYasser E IbrahimYounis K HamadPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254170 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amgad A Saleh Anwar H Sharafaddin Mahmoud H El Komy Yasser E Ibrahim Younis K Hamad Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
description	Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of different temperatures on mycelial growth of these strains. The pathogenicity of four strains of F. proliferatum was also evaluated on local date palm cultivars. Eleven SSR markers amplified a total of 57 scorable alleles from Fusarium strains. Phylogenetic analysis showed that F. proliferatum strains grouped in one clade with 95% bootstrap value. Within F. proliferatum clade, 14 SSR genotypes were identified, 9 of them were singleton. Four out of the five multi-individual SSR genotypes contained strains isolated from more than one location. Most F. solani strains grouped in one clade with 95% bootstrap value. Overall, the SSR markers previously developed for F. verticillioides and F. oxysporum were very useful in assessing the genetic diversity and confirming the identity of Saudi Fusarium strains. The results from the temperature study showed significant differences in mycelial growth of Fusarium strains at different temperatures tested. The highest average radial growth for Fusarium strains was observed at 25°C, irrespective of species. The four F. proliferatum strains showed significant differences in their pathogenicity on date palm cultivars. It is anticipated that the assessment of genetic diversity, effect of temperature on hyphal growth and pathogenicity of potent pathogenic Fusarium strains recovered from date palm-growing locations in Saudi Arabia can help in effectively controlling these pathogens.
format	article
author	Amgad A Saleh Anwar H Sharafaddin Mahmoud H El Komy Yasser E Ibrahim Younis K Hamad
author_facet	Amgad A Saleh Anwar H Sharafaddin Mahmoud H El Komy Yasser E Ibrahim Younis K Hamad
author_sort	Amgad A Saleh
title	Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
title_short	Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
title_full	Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
title_fullStr	Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
title_full_unstemmed	Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.
title_sort	molecular and physiological characterization of fusarium strains associated with different diseases in date palm.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6607a16276ea449fa43c4c8840c48da8
work_keys_str_mv	AT amgadasaleh molecularandphysiologicalcharacterizationoffusariumstrainsassociatedwithdifferentdiseasesindatepalm AT anwarhsharafaddin molecularandphysiologicalcharacterizationoffusariumstrainsassociatedwithdifferentdiseasesindatepalm AT mahmoudhelkomy molecularandphysiologicalcharacterizationoffusariumstrainsassociatedwithdifferentdiseasesindatepalm AT yassereibrahim molecularandphysiologicalcharacterizationoffusariumstrainsassociatedwithdifferentdiseasesindatepalm AT youniskhamad molecularandphysiologicalcharacterizationoffusariumstrainsassociatedwithdifferentdiseasesindatepalm
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Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm.

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