Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface

Abstract There is an urgent need to develop improved, physiologically-relevant in vitro models of airway epithelia with which to better understand the pathological processes associated with infection, allergies and toxicological insults of the respiratory tract of both humans and domesticated animal...

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Autores principales:	Daniel Cozens, Erin Sutherland, Francesco Marchesi, Geraldine Taylor, Catherine C. Berry, Robert L. Davies
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Native Airway Epithelium Bovine Bronchial Epithelial Cells (BBEC) Excellent Mimic Pseudostratified Epithelium Transepithelial Electrical Resistance (TEER) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/660bb38903bd4c6995c7d81d0f39ddaf
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spelling	oai:doaj.org-article:660bb38903bd4c6995c7d81d0f39ddaf2021-12-02T11:40:37ZTemporal differentiation of bovine airway epithelial cells grown at an air-liquid interface10.1038/s41598-018-33180-w2045-2322https://doaj.org/article/660bb38903bd4c6995c7d81d0f39ddaf2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33180-whttps://doaj.org/toc/2045-2322Abstract There is an urgent need to develop improved, physiologically-relevant in vitro models of airway epithelia with which to better understand the pathological processes associated with infection, allergies and toxicological insults of the respiratory tract of both humans and domesticated animals. In the present study, we have characterised the proliferation and differentiation of primary bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface (ALI) at three-day intervals over a period of 42 days from the introduction of the ALI. The differentiated BBEC model was highly representative of the ex vivo epithelium from which the epithelial cells were derived; a columnar, pseudostratified epithelium that was highly reflective of native airway epithelium was formed which comprised ciliated, goblet and basal cells. The hallmark defences of the respiratory tract, namely barrier function and mucociliary clearance, were present, thus demonstrating that the model is an excellent mimic of bovine respiratory epithelium. The epithelium was fully differentiated by day 21 post-ALI and, crucially, remained healthy and stable for a further 21 days. Thus, the differentiated BBEC model has a three-week window which will allow wide-ranging and long-term experiments to be performed in the fields of infection, toxicology or general airway physiology.Daniel CozensErin SutherlandFrancesco MarchesiGeraldine TaylorCatherine C. BerryRobert L. DaviesNature PortfolioarticleNative Airway EpitheliumBovine Bronchial Epithelial Cells (BBEC)Excellent MimicPseudostratified EpitheliumTransepithelial Electrical Resistance (TEER)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Native Airway Epithelium Bovine Bronchial Epithelial Cells (BBEC) Excellent Mimic Pseudostratified Epithelium Transepithelial Electrical Resistance (TEER) Medicine R Science Q
spellingShingle	Native Airway Epithelium Bovine Bronchial Epithelial Cells (BBEC) Excellent Mimic Pseudostratified Epithelium Transepithelial Electrical Resistance (TEER) Medicine R Science Q Daniel Cozens Erin Sutherland Francesco Marchesi Geraldine Taylor Catherine C. Berry Robert L. Davies Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
description	Abstract There is an urgent need to develop improved, physiologically-relevant in vitro models of airway epithelia with which to better understand the pathological processes associated with infection, allergies and toxicological insults of the respiratory tract of both humans and domesticated animals. In the present study, we have characterised the proliferation and differentiation of primary bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface (ALI) at three-day intervals over a period of 42 days from the introduction of the ALI. The differentiated BBEC model was highly representative of the ex vivo epithelium from which the epithelial cells were derived; a columnar, pseudostratified epithelium that was highly reflective of native airway epithelium was formed which comprised ciliated, goblet and basal cells. The hallmark defences of the respiratory tract, namely barrier function and mucociliary clearance, were present, thus demonstrating that the model is an excellent mimic of bovine respiratory epithelium. The epithelium was fully differentiated by day 21 post-ALI and, crucially, remained healthy and stable for a further 21 days. Thus, the differentiated BBEC model has a three-week window which will allow wide-ranging and long-term experiments to be performed in the fields of infection, toxicology or general airway physiology.
format	article
author	Daniel Cozens Erin Sutherland Francesco Marchesi Geraldine Taylor Catherine C. Berry Robert L. Davies
author_facet	Daniel Cozens Erin Sutherland Francesco Marchesi Geraldine Taylor Catherine C. Berry Robert L. Davies
author_sort	Daniel Cozens
title	Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
title_short	Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
title_full	Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
title_fullStr	Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
title_full_unstemmed	Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
title_sort	temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/660bb38903bd4c6995c7d81d0f39ddaf
work_keys_str_mv	AT danielcozens temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface AT erinsutherland temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface AT francescomarchesi temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface AT geraldinetaylor temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface AT catherinecberry temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface AT robertldavies temporaldifferentiationofbovineairwayepithelialcellsgrownatanairliquidinterface
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Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface

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