Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.

Retinoic acid (RA) plays major roles during nervous system development, and during regeneration of the adult nervous system. We have previously shown that components of the RA signaling pathway are upregulated after optic nerve injury, and that exogenous application of all-trans retinoic acid (ATRA)...

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Autores principales:	Valeria De La Rosa-Reyes, Mildred V Duprey-Díaz, Jonathan M Blagburn, Rosa E Blanco
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/660d8313c02a4ca9805a3e5534d908c4
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spelling	oai:doaj.org-article:660d8313c02a4ca9805a3e5534d908c42021-12-02T20:06:06ZRetinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.1932-620310.1371/journal.pone.0255196https://doaj.org/article/660d8313c02a4ca9805a3e5534d908c42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255196https://doaj.org/toc/1932-6203Retinoic acid (RA) plays major roles during nervous system development, and during regeneration of the adult nervous system. We have previously shown that components of the RA signaling pathway are upregulated after optic nerve injury, and that exogenous application of all-trans retinoic acid (ATRA) greatly increases the survival of axotomized retinal ganglion cells (RGCs). The objective of the present study is to investigate the effects of ATRA application on the macrophages in the optic nerve after injury, and to determine whether this affects axonal regeneration. The optic nerve was crushed and treated with PBS, ATRA and/or clodronate-loaded liposomes. Nerves were examined at one and two weeks after axotomy with light microscopy, immunocytochemistry and electron microscopy. ATRA application to the optic nerve caused transient increases in the number of macrophages and microglia one week after injury. The macrophages are consistently labeled with M2-type markers, and have considerable phagocytic activity. ATRA increased ultrastructural features of ongoing phagocytic activity in macrophages at one and two weeks. ATRA treatment also significantly increased the numbers of regenerating GAP-43-labeled axons. Clodronate liposome treatment depleted macrophage numbers by 80%, completely eliminated the ATRA-mediated increase in axonal regeneration, and clodronate treatment alone decreased axonal numbers by 30%. These results suggest that the success of axon regeneration is partially dependent on the presence of debris-phagocytosing macrophages, and that the increases in regeneration caused by ATRA are in part due to their increased numbers. Further studies will examine whether macrophage depletion affects RGC survival.Valeria De La Rosa-ReyesMildred V Duprey-DíazJonathan M BlagburnRosa E BlancoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0255196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria De La Rosa-Reyes Mildred V Duprey-Díaz Jonathan M Blagburn Rosa E Blanco Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
description	Retinoic acid (RA) plays major roles during nervous system development, and during regeneration of the adult nervous system. We have previously shown that components of the RA signaling pathway are upregulated after optic nerve injury, and that exogenous application of all-trans retinoic acid (ATRA) greatly increases the survival of axotomized retinal ganglion cells (RGCs). The objective of the present study is to investigate the effects of ATRA application on the macrophages in the optic nerve after injury, and to determine whether this affects axonal regeneration. The optic nerve was crushed and treated with PBS, ATRA and/or clodronate-loaded liposomes. Nerves were examined at one and two weeks after axotomy with light microscopy, immunocytochemistry and electron microscopy. ATRA application to the optic nerve caused transient increases in the number of macrophages and microglia one week after injury. The macrophages are consistently labeled with M2-type markers, and have considerable phagocytic activity. ATRA increased ultrastructural features of ongoing phagocytic activity in macrophages at one and two weeks. ATRA treatment also significantly increased the numbers of regenerating GAP-43-labeled axons. Clodronate liposome treatment depleted macrophage numbers by 80%, completely eliminated the ATRA-mediated increase in axonal regeneration, and clodronate treatment alone decreased axonal numbers by 30%. These results suggest that the success of axon regeneration is partially dependent on the presence of debris-phagocytosing macrophages, and that the increases in regeneration caused by ATRA are in part due to their increased numbers. Further studies will examine whether macrophage depletion affects RGC survival.
format	article
author	Valeria De La Rosa-Reyes Mildred V Duprey-Díaz Jonathan M Blagburn Rosa E Blanco
author_facet	Valeria De La Rosa-Reyes Mildred V Duprey-Díaz Jonathan M Blagburn Rosa E Blanco
author_sort	Valeria De La Rosa-Reyes
title	Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
title_short	Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
title_full	Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
title_fullStr	Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
title_full_unstemmed	Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.
title_sort	retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog rana pipiens.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/660d8313c02a4ca9805a3e5534d908c4
work_keys_str_mv	AT valeriadelarosareyes retinoicacidtreatmentrecruitsmacrophagesandincreasesaxonalregenerationafteropticnerveinjuryinthefrogranapipiens AT mildredvdupreydiaz retinoicacidtreatmentrecruitsmacrophagesandincreasesaxonalregenerationafteropticnerveinjuryinthefrogranapipiens AT jonathanmblagburn retinoicacidtreatmentrecruitsmacrophagesandincreasesaxonalregenerationafteropticnerveinjuryinthefrogranapipiens AT rosaeblanco retinoicacidtreatmentrecruitsmacrophagesandincreasesaxonalregenerationafteropticnerveinjuryinthefrogranapipiens
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Retinoic acid treatment recruits macrophages and increases axonal regeneration after optic nerve injury in the frog Rana pipiens.

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